Hydrophobic Probe Binding of β-Lactoglobulin in the Native and Molten Globule State Induced by High Pressure as Affected by pH, KIO3andN-Ethylmaleimide

2002 ◽  
Vol 50 (18) ◽  
pp. 5207-5214 ◽  
Author(s):  
Jian Yang ◽  
Joseph R. Powers ◽  
Stephanie Clark ◽  
A. Keith Dunker ◽  
Barry G. Swanson
Keyword(s):  
High Pressure ◽  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin ◽  
Hydrophobic Probe

β-Lactoglobulin Molten Globule Induced by High Pressure

2001 ◽  
Vol 49 (7) ◽  
pp. 3236-3243 ◽  
Author(s):  
Jian Yang ◽  
A. Keith Dunker ◽  
Joseph R. Powers ◽  
Stephanie Clark ◽  
Barry G. Swanson
Keyword(s):  
High Pressure ◽  
Molten Globule ◽  
Β Lactoglobulin

Molten globule state of equine β-Lactoglobulin

1997 ◽  
Vol 27 (4) ◽  
pp. 567-575 ◽  
Author(s):  
Masamichi Ikeguchi ◽  
Shin-ichi Kato ◽  
Akio Shimizu ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin

Interactions between aroma compounds and β-lactoglobulin in the heat-induced molten globule state

2010 ◽  
Vol 119 (4) ◽  
pp. 1550-1556 ◽  
Author(s):  
Laurette Tavel ◽  
Céline Moreau ◽  
Saïd Bouhallab ◽  
Eunice C.Y. Li-Chan ◽  
Elisabeth Guichard
Keyword(s):  
Molten Globule ◽  
Aroma Compounds ◽  
Molten Globule State ◽  
Β Lactoglobulin

Folding−Unfolding Equilibrium and Kinetics of Equine β-Lactoglobulin:  Equivalence between the Equilibrium Molten Globule State and a Burst-Phase Folding Intermediate†

Biochemistry ◽  
1999 ◽  
Vol 38 (14) ◽  
pp. 4455-4463 ◽  
Author(s):  
Kazuo Fujiwara ◽  
Munehito Arai ◽  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Kunihiro Kuwajima ◽  
...  
Keyword(s):  
Molten Globule ◽  
Folding Intermediate ◽  
Molten Globule State ◽  
Equilibrium And Kinetics ◽  
Burst Phase ◽  
Β Lactoglobulin ◽  
Kinetics Of

Proline Scanning Mutagenesis Reveals Non-Native Fold in the Molten Globule State of Equine β-Lactoglobulin†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15468-15473 ◽  
Author(s):  
Kanako Nakagawa ◽  
Akihito Tokushima ◽  
Kazuo Fujiwara ◽  
Masamichi Ikeguchi
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin ◽  
Native Fold

scholarly journals Molten globule as an intermediate on the human prostatic phosphatase folding pathway.

1997 ◽  
Vol 44 (4) ◽  
pp. 645-657 ◽  
Author(s):  
R Kuciel ◽  
A Mazurkiewicz
Keyword(s):  
Chromatographic Analysis ◽  
Molten Globule ◽  
Prostatic Acid Phosphatase ◽  
Water Soluble ◽  
Folding Pathway ◽  
Molten Globule State ◽  
Folding Intermediates ◽  
Beta Structure ◽  
Lipid Structures ◽  
Hydrophobic Probe

Human prostatic acid phosphatase (hPAP, EC.3.1.3.2), a secretory homodimeric protein was denatured in 6 M urea, pH 2.5, and refolded by dilution at pH 7.2 with recovery of the enzymatic activity and dimeric structure. Circular dichroism, intrinsic fluorescence and chromatographic analysis of renaturating protein suggested that the kinetic intermediate of the hPAP folding is a monomer which displays a molten globule state (R. Kuciel, A. Mazurkiewicz & W.S. Ostrowski, 1996, Int. J. Biol. Macromol. 18, 167-175). To confirm these data experiments were performed to estimate the interaction of the renaturating protein with dyes and amphipathic lipid structures. Increased binding of the hydrophobic probe 1-anilinonaphthalene-8-sulfonate and Congo Red to the refolding enzyme supported the existence of molten globule state with the relaxed beta-structure in the renaturating protein. Presence of liposomes, included in the renaturation mixture as a model of acid phospholipid, resulted in perturbations of the human PAP refolding process. Some folding intermediates were bound to phosphatidylserine liposomes or, alternatively, water soluble, inactive aggregates were formed.

Unfolding of the molten globule state of .alpha.-lactalbumin studied by proton NMR

Biochemistry ◽  
1993 ◽  
Vol 32 (48) ◽  
pp. 13198-13203 ◽  
Author(s):  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Proton Nmr ◽  
Molten Globule State ◽  
Alpha Lactalbumin

Time-resolved Fluorescence Studies of the Molten Globule State of Apomyoglobin

1996 ◽  
Vol 257 (4) ◽  
pp. 877-885 ◽  
Author(s):  
Christian Rischel ◽  
Per Thyberg ◽  
Rudolf Rigler ◽  
Flemming M. Poulsen
Keyword(s):  
Molten Globule ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Molten Globule State ◽  
Fluorescence Studies
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