Proline Scanning Mutagenesis Reveals Non-Native Fold in the Molten Globule State of Equine β-Lactoglobulin†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15468-15473 ◽  
Author(s):  
Kanako Nakagawa ◽  
Akihito Tokushima ◽  
Kazuo Fujiwara ◽  
Masamichi Ikeguchi
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin ◽  
Native Fold

Molten globule state of equine β-Lactoglobulin

1997 ◽  
Vol 27 (4) ◽  
pp. 567-575 ◽  
Author(s):  
Masamichi Ikeguchi ◽  
Shin-ichi Kato ◽  
Akio Shimizu ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin

Interactions between aroma compounds and β-lactoglobulin in the heat-induced molten globule state

2010 ◽  
Vol 119 (4) ◽  
pp. 1550-1556 ◽  
Author(s):  
Laurette Tavel ◽  
Céline Moreau ◽  
Saïd Bouhallab ◽  
Eunice C.Y. Li-Chan ◽  
Elisabeth Guichard
Keyword(s):  
Molten Globule ◽  
Aroma Compounds ◽  
Molten Globule State ◽  
Β Lactoglobulin

Folding−Unfolding Equilibrium and Kinetics of Equine β-Lactoglobulin:  Equivalence between the Equilibrium Molten Globule State and a Burst-Phase Folding Intermediate†

Biochemistry ◽  
1999 ◽  
Vol 38 (14) ◽  
pp. 4455-4463 ◽  
Author(s):  
Kazuo Fujiwara ◽  
Munehito Arai ◽  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Kunihiro Kuwajima ◽  
...  
Keyword(s):  
Molten Globule ◽  
Folding Intermediate ◽  
Molten Globule State ◽  
Equilibrium And Kinetics ◽  
Burst Phase ◽  
Β Lactoglobulin ◽  
Kinetics Of

Unfolding of the molten globule state of .alpha.-lactalbumin studied by proton NMR

Biochemistry ◽  
1993 ◽  
Vol 32 (48) ◽  
pp. 13198-13203 ◽  
Author(s):  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Proton Nmr ◽  
Molten Globule State ◽  
Alpha Lactalbumin

Time-resolved Fluorescence Studies of the Molten Globule State of Apomyoglobin

1996 ◽  
Vol 257 (4) ◽  
pp. 877-885 ◽  
Author(s):  
Christian Rischel ◽  
Per Thyberg ◽  
Rudolf Rigler ◽  
Flemming M. Poulsen
Keyword(s):  
Molten Globule ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Molten Globule State ◽  
Fluorescence Studies

scholarly journals The molten globule state of α‐lactalbumin

1996 ◽  
Vol 10 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Kunihiro Kuwajima
Keyword(s):  
Molten Globule ◽  
Molten Globule State

Effect of curcumin on amyloidogenic property of molten globule-like intermediate state of 2,5-diketo-d-gluconate reductase A

2009 ◽  
Vol 390 (10) ◽  
Author(s):  
Nandini Sarkar ◽  
Abhay Narain Singh ◽  
Vikash Kumar Dubey
Keyword(s):  
Protein Concentration ◽  
Molten Globule ◽  
Molar Ratio ◽  
Amyloid Formation ◽  
Molten Globule State ◽  
Force Microscopy ◽  
Atomic Force ◽  
Sheet Structure ◽  
Amyloid Diseases ◽  
Β Sheet

Abstract We identified a molten globule-like intermediate of 2,5-diketo-d-gluconate reductase A (DKGR) at pH 2.5, which has a prominent β-sheet structure. The molten globule state of the protein shows amyloidogenic property >50 μm protein concentration. Interestingly, a 1:1 molar ratio of curcumin prevents amyloid formation as shown by the Thioflavin-T assay and atomic force microscopy. To the best of our knowledge, this is the first report on amyloid formation by an (α/β)8-barrel protein. The results presented here indicate that the molten globule state has an important role in amyloid formation and potential application of curcumin in protein biotechnology as well as therapeutics against amyloid diseases.

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