Folding−Unfolding Equilibrium and Kinetics of Equine β-Lactoglobulin:  Equivalence between the Equilibrium Molten Globule State and a Burst-Phase Folding Intermediate†

Biochemistry ◽  
1999 ◽  
Vol 38 (14) ◽  
pp. 4455-4463 ◽  
Author(s):  
Kazuo Fujiwara ◽  
Munehito Arai ◽  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Kunihiro Kuwajima ◽  
...  
Keyword(s):  
Molten Globule ◽  
Folding Intermediate ◽  
Molten Globule State ◽  
Equilibrium And Kinetics ◽  
Burst Phase ◽  
Β Lactoglobulin ◽  
Kinetics Of

Kinetic and equilibrium folding intermediates

1995 ◽  
Vol 348 (1323) ◽  
pp. 35-41 ◽  
Keyword(s):  
Protein Folding ◽  
Molten Globule ◽  
Low Dielectric Constant ◽  
The Novel ◽  
Folding Intermediate ◽  
Molten Globule State ◽  
Folding Intermediates ◽  
First Order ◽  
Low Dielectric ◽  
First Order Phase

Our recent experiments on the molten globule state and other protein folding intermediates lead to following conclusions: (i) the molten globule is separated by intramolecular first-order phase transitions from the native and unfolded states and therefore is a specific thermodynamic state of protein molecules; (ii) the novel equilibrium folding intermediate (the ‘pre-molten globule’ state) exists which can be similar to the ‘burst’ kinetic intermediate of protein folding; (iii) proteins denature and release their non-polar ligands at moderately low pH and moderately low dielectric constant, i.e. under conditions which may be related to those near membranes.

Molten globule state of equine β-Lactoglobulin

1997 ◽  
Vol 27 (4) ◽  
pp. 567-575 ◽  
Author(s):  
Masamichi Ikeguchi ◽  
Shin-ichi Kato ◽  
Akio Shimizu ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin

scholarly journals Folding mechanism of canine milk lysozyme studied by circular dichroism and fluorescence spectroscopy

2003 ◽  
Vol 17 (2-3) ◽  
pp. 183-193 ◽  
Author(s):  
Masaharu Nakao ◽  
Munehito Arai ◽  
Takumi Koshiba ◽  
Katsutoshi Nitta ◽  
Kunihiro Kuwajima
Keyword(s):  
Circular Dichroism ◽  
Fluorescence Spectroscopy ◽  
Molten Globule ◽  
Guanidine Hydrochloride ◽  
Folding Intermediates ◽  
Equilibrium Unfolding ◽  
Burst Phase ◽  
The Relationship ◽  
Kinetics Of ◽  
Circular Dichroïsm

We have studied the guanidine hydrochloride‒induced equilibrium unfolding and the kinetics of refolding of canine milk lysozyme by circular dichroism and fluorescence spectroscopy. The thermodynamic analysis of the equilibrium unfolding measured by circular dichroism and fluorescence has shown that unfolding is represented by a three‒state mechanism and that the intermediate state of canine milk lysozyme is remarkably more stable than the intermediates observed in other lysozyme and α-lactalbumin. In the kinetic refolding of this protein, there are at least two kinetic intermediates; a burst=phase intermediate accumulated within the dead time (4 ms) of the measurement and an intermediate that has been observed during the kinetics with a rate constant of 10–20 s–1after the burst phase. This result is apparently in contrast with those previously observed in the kinetic refolding of α‒lactalbumin and equine lysozyme that show only the burst‒phase intermediate. The relationship between the extraordinarily stable equilibrium molten globule and the kinetic folding intermediates will be discussed.

Interactions between aroma compounds and β-lactoglobulin in the heat-induced molten globule state

2010 ◽  
Vol 119 (4) ◽  
pp. 1550-1556 ◽  
Author(s):  
Laurette Tavel ◽  
Céline Moreau ◽  
Saïd Bouhallab ◽  
Eunice C.Y. Li-Chan ◽  
Elisabeth Guichard
Keyword(s):  
Molten Globule ◽  
Aroma Compounds ◽  
Molten Globule State ◽  
Β Lactoglobulin

scholarly journals Hydrogen exchange study of folding intermediate and molten globule state of canine milk lysozyme

2000 ◽  
Vol 40 (supplement) ◽  
pp. S160
Author(s):  
Y. Kobashigawa ◽  
M Mizuguchi ◽  
M. Demura ◽  
T. Koshiba ◽  
S. Tsuda ◽  
...  
Keyword(s):  
Hydrogen Exchange ◽  
Molten Globule ◽  
Folding Intermediate ◽  
Molten Globule State ◽  
Exchange Study

Proline Scanning Mutagenesis Reveals Non-Native Fold in the Molten Globule State of Equine β-Lactoglobulin†

Biochemistry ◽  
2006 ◽  
Vol 45 (51) ◽  
pp. 15468-15473 ◽  
Author(s):  
Kanako Nakagawa ◽  
Akihito Tokushima ◽  
Kazuo Fujiwara ◽  
Masamichi Ikeguchi
Keyword(s):  
Molten Globule ◽  
Molten Globule State ◽  
Β Lactoglobulin ◽  
Native Fold

Adsorption Equilibrium and Kinetics of CO2 and H2O on Activated Carbon

2012 ◽  
Vol 27 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Dong XU ◽  
Jun ZHANG ◽  
Gang LI ◽  
Penny XIAO ◽  
Paul WEBLEY ◽  
...  
Keyword(s):  
Activated Carbon ◽  
Adsorption Equilibrium ◽  
Equilibrium And Kinetics ◽  
Adsorption Equilibrium And Kinetics ◽  
Kinetics Of

Unfolding of the molten globule state of .alpha.-lactalbumin studied by proton NMR

Biochemistry ◽  
1993 ◽  
Vol 32 (48) ◽  
pp. 13198-13203 ◽  
Author(s):  
Akio Shimizu ◽  
Masamichi Ikeguchi ◽  
Shintaro Sugai
Keyword(s):  
Molten Globule ◽  
Proton Nmr ◽  
Molten Globule State ◽  
Alpha Lactalbumin
Sign in / Sign up

Export Citation Format

Share Document