Mass Spectrometry Characterization of Higher Order Structural Changes Associated with the Fc-glycan Structure of the NISTmAb Reference Material, RM 8761

2020 ◽  
Vol 31 (3) ◽  
pp. 553-564 ◽  
Author(s):  
Kate Groves ◽  
Adam Cryar ◽  
Simon Cowen ◽  
Alison E. Ashcroft ◽  
Milena Quaglia
Keyword(s):  
Mass Spectrometry ◽  
Reference Material ◽  
Structural Changes ◽  
Higher Order ◽  
Glycan Structure

scholarly journals Characterization of a New Certified Reference Material for Human Cardiac Troponin I

2006 ◽  
Vol 52 (2) ◽  
pp. 212-219 ◽  
Author(s):  
David M Bunk ◽  
Michael J Welch
Keyword(s):  
Mass Spectrometry ◽  
Reference Material ◽  
Certified Reference Material ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Reversed Phase ◽  
Cardiac Troponin I ◽  
Reference Concentration ◽  
System Of Units

Abstract Background: To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new certified reference material, Standard Reference Material (SRM) 2921: Human Cardiac Troponin Complex. Methods: The concentration of cTnI in SRM 2921 was determined through a combination of reversed-phase liquid chromatography (LC) with ultraviolet detection and amino acid analysis. Characterization of the intact troponin subunits was accomplished through reversed-phase LC coupled with mass spectrometry. Posttranslational modifications to the cTnI in SRM 2921 were investigated by combining proteolytic digestion with matrix-assisted laser desorption/ionization mass spectrometry. Additionally, reference concentration values for cTnT and cTnC were also determined. Results: The concentration of human cTnI in SRM 2921 is 31.2 (1.4) mg/L (where 1.4 mg/L is the uncertainty at a 95% level of confidence), as certified through a method that provides traceability to the International System of Units (SI). Reference concentration values of the cTnT and cTnC subunits were determined to be 36.9 (3.8) mg/L and 24.2 (1.3) mg/L, respectively. Conclusions: This first cTnI reference material should provide SI traceability to clinical cTnI assays once commutability has been validated, and could assist in the international harmonization of cTnI assays as a tool for understanding the underlying causes of interassay variability.

scholarly journals Higher-order structural interrogation of antibodies using middle-down hydrogen/deuterium exchange mass spectrometry

2016 ◽  
Vol 7 (2) ◽  
pp. 1480-1486 ◽  
Author(s):  
Jingxi Pan ◽  
Suping Zhang ◽  
Albert Chou ◽  
Christoph H. Borchers
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Structural Characterization ◽  
Higher Order ◽  
Subzero Temperature ◽  
Hydrogen Deuterium Exchange ◽  
Exchange Mass Spectrometry ◽  
Hydrogen Deuterium ◽  
Deuterium Exchange Mass Spectrometry

Specific restricted proteolysis combined with subzero temperature HPLC and online ETD facilitates structural characterization of antibodies at high resolution.

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