Surface characterization of a National Bureau of Standards glass reference material by californium-252 particle desorption mass spectrometry

1988 ◽  
Vol 60 (18) ◽  
pp. 1944-1947 ◽  
Author(s):  
W. R. Summers ◽  
M. U. D. Beug-Deeb ◽  
E. A. Schweikert
Keyword(s):  
Mass Spectrometry ◽  
Reference Material ◽  
Surface Characterization ◽  
National Bureau ◽  
Desorption Mass Spectrometry

scholarly journals Characterization of a New Certified Reference Material for Human Cardiac Troponin I

2006 ◽  
Vol 52 (2) ◽  
pp. 212-219 ◽  
Author(s):  
David M Bunk ◽  
Michael J Welch
Keyword(s):  
Mass Spectrometry ◽  
Reference Material ◽  
Certified Reference Material ◽  
Cardiac Troponin ◽  
Troponin I ◽  
Reversed Phase ◽  
Cardiac Troponin I ◽  
Reference Concentration ◽  
System Of Units

Abstract Background: To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new certified reference material, Standard Reference Material (SRM) 2921: Human Cardiac Troponin Complex. Methods: The concentration of cTnI in SRM 2921 was determined through a combination of reversed-phase liquid chromatography (LC) with ultraviolet detection and amino acid analysis. Characterization of the intact troponin subunits was accomplished through reversed-phase LC coupled with mass spectrometry. Posttranslational modifications to the cTnI in SRM 2921 were investigated by combining proteolytic digestion with matrix-assisted laser desorption/ionization mass spectrometry. Additionally, reference concentration values for cTnT and cTnC were also determined. Results: The concentration of human cTnI in SRM 2921 is 31.2 (1.4) mg/L (where 1.4 mg/L is the uncertainty at a 95% level of confidence), as certified through a method that provides traceability to the International System of Units (SI). Reference concentration values of the cTnT and cTnC subunits were determined to be 36.9 (3.8) mg/L and 24.2 (1.3) mg/L, respectively. Conclusions: This first cTnI reference material should provide SI traceability to clinical cTnI assays once commutability has been validated, and could assist in the international harmonization of cTnI assays as a tool for understanding the underlying causes of interassay variability.

Sign in / Sign up

Export Citation Format

Share Document