Higher-order structural interrogation of antibodies using middle-down hydrogen/deuterium exchange mass spectrometry

Jingxi Pan; Suping Zhang; Albert Chou; Christoph H. Borchers

doi:10.1039/c5sc03420e

Higher-order structural interrogation of antibodies using middle-down hydrogen/deuterium exchange mass spectrometry

Chemical Science ◽

10.1039/c5sc03420e ◽

2016 ◽

Vol 7 (2) ◽

pp. 1480-1486 ◽

Cited By ~ 13

Author(s):

Jingxi Pan ◽

Suping Zhang ◽

Albert Chou ◽

Christoph H. Borchers

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

Structural Characterization ◽

Higher Order ◽

Subzero Temperature ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

Specific restricted proteolysis combined with subzero temperature HPLC and online ETD facilitates structural characterization of antibodies at high resolution.

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Structural Characterization of Impurities and Degradation Products in Pharmaceuticals Using High-Resolution LC-MS and Online Hydrogen/Deuterium Exchange Mass Spectrometry

Characterization of Impurities and Degradants Using Mass Spectrometry ◽

10.1002/9780470921371.ch5 ◽

2011 ◽

pp. 135-181

Author(s):

Guodong Chen ◽

Birendra N. Pramanik

Keyword(s):

Mass Spectrometry ◽

High Resolution ◽

Structural Characterization ◽

Degradation Products ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Higher order structure characterization of protein therapeutics by hydrogen/deuterium exchange mass spectrometry

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-014-7924-3 ◽

2014 ◽

Vol 406 (26) ◽

pp. 6541-6558 ◽

Cited By ~ 57

Author(s):

Richard Y.-C. Huang ◽

Guodong Chen

Keyword(s):

Mass Spectrometry ◽

Higher Order ◽

Protein Therapeutics ◽

Structure Characterization ◽

Order Structure ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Structural Characterization of Histone Octamers at Varied Salt Concentrations Using Hydrogen‐Deuterium Exchange Mass Spectrometry

The FASEB Journal ◽

10.1096/fasebj.2020.34.s1.06177 ◽

2020 ◽

Vol 34 (S1) ◽

pp. 1-1

Author(s):

Adam Timmerman

Keyword(s):

Mass Spectrometry ◽

Structural Characterization ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Histone Octamers

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Structural Characterization of Degrader-Induced Ternary Complexes Using Hydrogen–Deuterium Exchange Mass Spectrometry and Computational Modeling: Implications for Structure-Based Design

ACS Chemical Biology ◽

10.1021/acschembio.1c00376 ◽

2021 ◽

Author(s):

Scott J. Eron ◽

Hongwei Huang ◽

Roman V. Agafonov ◽

Mark E. Fitzgerald ◽

Joe Patel ◽

...

Keyword(s):

Mass Spectrometry ◽

Computational Modeling ◽

Structural Characterization ◽

Ternary Complexes ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Cover Picture: Identification of Pharmacological Chaperones for Gaucher Disease and Characterization of Their Effects on β-Glucocerebrosidase by Hydrogen/Deuterium Exchange Mass Spectrometry (ChemBioChem 16/2008)

ChemBioChem ◽

10.1002/cbic.200890061 ◽

2008 ◽

Vol 9 (16) ◽

pp. 2549-2549

Author(s):

Michael B. Tropak ◽

Gregory J. Kornhaber ◽

Brigitte A. Rigat ◽

Gustavo H. Maegawa ◽

Justin D. Buttner ◽

...

Keyword(s):

Mass Spectrometry ◽

Gaucher Disease ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Pharmacological Chaperones ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Picture Identification

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Hydrogen/deuterium exchange mass spectrometry for probing higher order structure of protein therapeutics: methodology and applications

Drug Discovery Today ◽

10.1016/j.drudis.2013.07.019 ◽

2014 ◽

Vol 19 (1) ◽

pp. 95-102 ◽

Cited By ~ 130

Author(s):

Hui Wei ◽

Jingjie Mo ◽

Li Tao ◽

Reb J. Russell ◽

Adrienne A. Tymiak ◽

...

Keyword(s):

Mass Spectrometry ◽

Higher Order ◽

Protein Therapeutics ◽

Deuterium Exchange ◽

Order Structure ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Analysis of Higher Order Structures of Proteins by Hydrogen Deuterium Exchange Mass Spectrometry

Journal of the Mass Spectrometry Society of Japan ◽

10.5702/massspec.s18-44 ◽

2018 ◽

Vol 66 (6) ◽

pp. 218-221

Author(s):

Tetsuo Torisu ◽

Hiroaki Oyama ◽

Susumu Uchiyama

Keyword(s):

Mass Spectrometry ◽

Higher Order ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry ◽

Order Structures

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Characterization of IgG1 Conformation and Conformational Dynamics by Hydrogen/Deuterium Exchange Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac802575y ◽

2009 ◽

Vol 81 (7) ◽

pp. 2644-2651 ◽

Cited By ~ 137

Author(s):

Damian Houde ◽

Joseph Arndt ◽

Wayne Domeier ◽

Steven Berkowitz ◽

John R. Engen

Keyword(s):

Mass Spectrometry ◽

Conformational Dynamics ◽

Deuterium Exchange ◽

Hydrogen Deuterium Exchange ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Subzero Temperature Chromatography for Reduced Back-Exchange and Improved Dynamic Range in Amide Hydrogen/Deuterium Exchange Mass Spectrometry

Analytical Chemistry ◽

10.1021/ac302488h ◽

2012 ◽

Vol 84 (21) ◽

pp. 9601-9608 ◽

Cited By ~ 25

Author(s):

John D. Venable ◽

Linda Okach ◽

Sanjay Agarwalla ◽

Ansgar Brock

Keyword(s):

Mass Spectrometry ◽

Dynamic Range ◽

Deuterium Exchange ◽

Subzero Temperature ◽

Hydrogen Deuterium Exchange ◽

Amide Hydrogen ◽

Exchange Mass Spectrometry ◽

Back Exchange ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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Comparative higher-order structure analysis of antibody biosimilars using combined bottom-up and top-down hydrogen-deuterium exchange mass spectrometry

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics ◽

10.1016/j.bbapap.2016.08.013 ◽

2016 ◽

Vol 1864 (12) ◽

pp. 1801-1808 ◽

Cited By ~ 19

Author(s):

Jingxi Pan ◽

Suping Zhang ◽

Christoph H. Borchers

Keyword(s):

Mass Spectrometry ◽

Structure Analysis ◽

Higher Order ◽

Deuterium Exchange ◽

Order Structure ◽

Hydrogen Deuterium Exchange ◽

Top Down ◽

Exchange Mass Spectrometry ◽

Hydrogen Deuterium ◽

Deuterium Exchange Mass Spectrometry

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