Real-Time Imaging of Single-Molecule Enzyme Cascade Using a DNA Origami Raft

2017 ◽  
Vol 139 (48) ◽  
pp. 17525-17532 ◽  
Author(s):  
Lele Sun ◽  
Yanjing Gao ◽  
Yan Xu ◽  
Jie Chao ◽  
Huajie Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Dna Origami ◽  
Real Time Imaging ◽  
Enzyme Cascade

scholarly journals Real-time tracking of single-molecule collagenase on native collagen and partially structured collagen-mimic substrates

2018 ◽  
Vol 54 (73) ◽  
pp. 10248-10251
Author(s):  
James Froberg ◽  
Woo-Sik Choi ◽  
Abbas Sedigh ◽  
Tayebeh Anajafi ◽  
Jasmin Farmakes ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Real Time Imaging ◽  
Native Collagen ◽  
Proteolytic Activities ◽  
Real Time Tracking

Real-time imaging and tracking of proteolytic activities of individual enzymes with their native and structurally modified substrates has been investigated.

scholarly journals Single-molecule DNA origami aptasensors for real-time biomarker detection

2020 ◽  
Vol 8 (30) ◽  
pp. 6352-6356
Author(s):  
Keitel Cervantes-Salguero ◽  
Mark Freeley ◽  
Jorge L. Chávez ◽  
Matteo Palma
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Conformational Changes ◽  
Dna Origami ◽  
Biomarker Detection ◽  
Dna Nanostructures

DNA nanostructures as platforms to monitor the inherent conformational changes of aptamers upon analyte binding, with single-molecule resolution and real-time capability.

Real-Time Imaging of Single-Molecule Fluorescence with a Zero-Mode Waveguide for the Analysis of Protein−Protein Interaction

2008 ◽  
Vol 80 (15) ◽  
pp. 6018-6022 ◽  
Author(s):  
Takeo Miyake ◽  
Takashi Tanii ◽  
Hironori Sonobe ◽  
Rena Akahori ◽  
Naonobu Shimamoto ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Protein Interaction ◽  
Zero Mode ◽  
Single Molecule Fluorescence ◽  
Real Time Imaging ◽  
Protein Protein Interaction

Monitoring patterned enzymatic polymerization on DNA origami at single-molecule level

Nanoscale ◽  
2015 ◽  
Vol 7 (25) ◽  
pp. 10970-10973 ◽  
Author(s):  
A. H. Okholm ◽  
H. Aslan ◽  
F. Besenbacher ◽  
M. Dong ◽  
J. Kjems
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Dna Origami ◽  
Enzymatic Polymerization ◽  
Dna Polymerization ◽  
Single Molecule Level ◽  
Site Selective

Site-selective enzymatic polymerization by TdT on surface-bound DNA origami embedded in BSA. DNA polymerization was visualized real time by AFM.

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