Direct-Write Patterning of Bacterial Cells by Dip-Pen Nanolithography

2012 ◽  
Vol 134 (40) ◽  
pp. 16500-16503 ◽  
Author(s):  
Jieun Kim ◽  
Young-Hun Shin ◽  
Seong-Hun Yun ◽  
Dong-Sik Choi ◽  
Ji-Hye Nam ◽  
...  
Keyword(s):  
Direct Write ◽  
Bacterial Cells ◽  
Dip Pen Nanolithography

Patterned Deposition of Nanoparticles Using Dip Pen Nanolithography For Synthesis of Carbon Nanotubes

2015 ◽  
Vol 1752 ◽  
pp. 65-70
Author(s):  
Kevin F. Dahlberg ◽  
Kelly Woods ◽  
Carol Jenkins ◽  
Christine C. Broadbridge ◽  
Todd C. Schwendemann
Keyword(s):  
Chemical Vapor ◽  
Growth Conditions ◽  
Direct Write ◽  
Patterned Substrate ◽  
Nanoparticle Catalysts ◽  
Analysis Process ◽  
Nucleation Sites ◽  
Wide Range ◽  
Synthesis Of Carbon Nanotubes ◽  
Dip Pen Nanolithography

AbstractOrdered carbon nanotube (CNT) growth by deposition of nanoparticle catalysts using dip pen nanolithography (DPN) is presented. DPN is a direct write, tip based lithography technique capable of multi-component deposition of a wide range of materials with nanometer precision. A NanoInk NLP 2000 is used to pattern different catalytic nanoparticle solutions on various substrates. To generate a uniform pattern of nanoparticle clusters, various conditions need to be considered. These parameters include: the humidity in the vessel, temperature, and tip-surface dwell time. By patterning different nanoparticle solutions next to each other, identical growth conditions can be compared for different catalysts in a streamlined analysis process. Fe, Ni, and Co nanoparticle solutions patterned on silicon, mica, and graphite substrates serve as nucleation sites for CNT growth. The CNTs were synthesized by a chemical vapor deposition (CVD) reaction. Each nanoparticle patterned substrate is placed in a tube furnace held at 725°C during CNT growth. The carbon source used in the growth chamber is toluene. The toluene is injected at a rate of 5 mL/hr. Growth is observed for Fe and Ni nanoparticle patterns, but is lacking for the Co patterns. The results of these reactions provide important information regarding efficient and highly reproducible mechanisms for CNT growth.

A Direct-Write Single-Step Positive Etch Resist for Dip-Pen Nanolithography

Small ◽  
2007 ◽  
Vol 3 (12) ◽  
pp. 2034-2037 ◽  
Author(s):  
Joseph H. Wei ◽  
David S. Ginger
Keyword(s):  
Single Step ◽  
Direct Write ◽  
Dip Pen Nanolithography

pH-Responsive Fluorescent Nanoarrays Fabricated by Direct-Write Parallel Dip-Pen Nanolithography

Small ◽  
2008 ◽  
Vol 4 (12) ◽  
pp. 2131-2135 ◽  
Author(s):  
Alberto Martínez-Otero ◽  
Jordi Hernando ◽  
Daniel Ruiz-Molina ◽  
Daniel Maspoch
Keyword(s):  
Direct Write ◽  
Ph Responsive ◽  
Dip Pen Nanolithography

Protein Nanostructures Formed via Direct-Write Dip-Pen Nanolithography

2003 ◽  
Vol 125 (19) ◽  
pp. 5588-5589 ◽  
Author(s):  
Ki-Bum Lee ◽  
Jung-Hyurk Lim ◽  
Chad A. Mirkin
Keyword(s):  
Direct Write ◽  
Dip Pen Nanolithography

scholarly journals Immobilization of motile bacterial cells via dip-pen nanolithography

2010 ◽  
Vol 21 (23) ◽  
pp. 235105 ◽  
Author(s):  
Dorjderem Nyamjav ◽  
Sergey Rozhok ◽  
Richard C Holz
Keyword(s):  
Bacterial Cells ◽  
Dip Pen Nanolithography

scholarly journals Direct-Write Dip-Pen Nanolithography of Proteins on Modified Silicon Oxide Surfaces

2003 ◽  
Vol 42 (20) ◽  
pp. 2309-2312 ◽  
Author(s):  
Jung-Hyurk Lim ◽  
David S. Ginger ◽  
Ki-Bum Lee ◽  
Jungseok Heo ◽  
Jwa-Min Nam ◽  
...  
Keyword(s):  
Silicon Oxide ◽  
Oxide Surfaces ◽  
Direct Write ◽  
Dip Pen Nanolithography

Effect of piperacillin on morphology and viability of gram-negative bacteria

1984 ◽  
Vol 42 ◽  
pp. 218-219
Author(s):  
R. H. Liss
Keyword(s):  
Inhibitory Concentration ◽  
Cytoplasmic Membrane ◽  
Drug Binding ◽  
Gram Negative Bacteria ◽  
Bacterial Cells ◽  
Drug Induced ◽  
Penicillin Binding Proteins ◽  
Lactam Antibiotic ◽  
Drug Free ◽  
Tube Dilution

Piperacillip (PIP) is b-[D(-)-α-(4-ethy1-2,3-dioxo-l-piperzinylcar-bonylamino)-α-phenylacetamido]-penicillanate. The broad spectrum semisynthetic β-lactam antibiotic is believed to effect bactericidal activity through its affinity for penicillin-binding proteins (PBPs), enzymes on the bacterial cytoplasmic membrane that control elongation and septation during cell growth and division. The purpose of this study was to correlate penetration and binding of 14C-PIP in bacterial cells with drug-induced lethal changes assessed by microscopic, microbiologic and biochemical methods.The bacteria used were clinical isolates of Escherichia coli and Pseudomonas aeruginosa (Figure 1). Sensitivity to the drug was determined by serial tube dilution in Trypticase Soy Broth (BBL) at an inoculum of 104 organisms/ml; the minimum inhibitory concentration of piperacillin for both bacteria was 1 μg/ml. To assess drug binding to PBPs, the bacteria were incubated with 14C-PIP (5 μg/0.09 μCi/ml); controls, in drug-free medium.

Attachment of oral bacteria to titanium surfaces: An SEM study

1994 ◽  
Vol 52 ◽  
pp. 468-469
Author(s):  
J. E. Laffoon ◽  
R. L. Anderson ◽  
J. C. Keller ◽  
C. D. Wu-Yuan
Keyword(s):  
Technical Problem ◽  
Titanium Implant ◽  
Oral Bacteria ◽  
Bacterial Cells ◽  
Thin Sections ◽  
Surface Ultrastructure ◽  
Sem Study ◽  
Key Factor ◽  
Titanium Surfaces

Titanium (Ti) dental implants have been used widely for many years. Long term implant failures are related, in part, to the development of peri-implantitis frequently associated with bacteria. Bacterial adherence and colonization have been considered a key factor in the pathogenesis of many biomaterial based infections. Without the initial attachment of oral bacteria to Ti-implant surfaces, subsequent polymicrobial accumulation and colonization leading to peri-implant disease cannot occur. The overall goal of this study is to examine the implant-oral bacterial interfaces and gain a greater understanding of their attachment characteristics and mechanisms. Since the detailed cell surface ultrastructure involved in attachment is only discernible at the electron microscopy level, the study is complicated by the technical problem of obtaining titanium implant and attached bacterial cells in the same ultra-thin sections. In this study, a technique was developed to facilitate the study of Ti implant-bacteria interface.Discs of polymerized Spurr’s resin (12 mm x 5 mm) were formed to a thickness of approximately 3 mm using an EM block holder (Fig. 1). Titanium was then deposited by vacuum deposition to a film thickness of 300Å (Fig. 2).

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