Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

ChemBioChem ◽  
2012 ◽  
Vol 13 (13) ◽  
pp. 1946-1952 ◽  
Author(s):  
Xiaoying Bian ◽  
Fan Huang ◽  
Francis A. Stewart ◽  
Liqiu Xia ◽  
Youming Zhang ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Direct Cloning

scholarly journals Genetic engineering and heterologous expression of the disorazol biosynthetic gene cluster via Red/ET recombineering

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Qiang Tu ◽  
Jennifer Herrmann ◽  
Shengbiao Hu ◽  
Ritesh Raju ◽  
Xiaoying Bian ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Heterologous Expression And Genetic Engineering of the Phenalinolactone Biosynthetic Gene Cluster by Using Red/ET Recombineering

ChemBioChem ◽  
2008 ◽  
Vol 9 (3) ◽  
pp. 447-454 ◽  
Author(s):  
Tina M. Binz ◽  
Silke C. Wenzel ◽  
Hans-Jörg Schnell ◽  
Andreas Bechthold ◽  
Rolf Müller
Keyword(s):  
Genetic Engineering ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

scholarly journals Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani

2020 ◽  
Vol 21 (20) ◽  
pp. 7601
Author(s):  
Tobias Bruun Pedersen ◽  
Mikkel Rank Nielsen ◽  
Sebastian Birkedal Kristensen ◽  
Eva Mie Lang Spedtsberg ◽  
Wafaa Yasmine ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Fusarium Solani ◽  
Biosynthetic Gene Cluster ◽  
Vector System ◽  
Biosynthetic Gene ◽  
The Core ◽  
Maximum Titer ◽  
Sequential Transformation ◽  
Core Genes

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

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