Controlled Photoinitiated Release of Nitric Oxide from Polymer Films ContainingS-Nitroso-N-acetyl-dl-penicillamine Derivatized Fumed Silica Filler

2004 ◽  
Vol 126 (5) ◽  
pp. 1348-1349 ◽  
Author(s):  
Megan C. Frost ◽  
Mark E. Meyerhoff
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Fumed Silica ◽  
Silica Filler

scholarly journals Development of Photoinitiated Nitric Oxide Releasing Polymer Films for Controlled Drug Delivery

2009 ◽  
Vol 3 (2) ◽  
Author(s):  
D. M. Smeenge ◽  
M. J. Barron ◽  
M. T. Nielsen ◽  
J. Goldman ◽  
M. C. Frost
Keyword(s):  
Nitric Oxide ◽  
Drug Delivery ◽  
Smooth Muscle ◽  
Cell Cultures ◽  
Polymer Films ◽  
Surface Flux ◽  
Surface Fluxes ◽  
No Donors ◽  
Polymeric Systems ◽  
Smooth Muscle Cell Cultures

Nitric Oxide (NO) is small, free radical gas that has been shown to have a wide variety of physiological functions, including the ability to hinder tumor angiogenesis at high, but non lethal, concentrations [1]. Previous work suggests that if NO could be effectively delivered in vivo to tumors of patients currently undergoing chemotherapy treatments at the appropriate levels, less damaging chemotherapy treatments could be used against cancer [2]. This could increase the overall survivability of cancer patients, especially in those prone to the harmful effects of chemotherapy: children, elderly, and those of weak immune systems. If NO is especially successful at preventing and eliminating tumor growth, angiogenesis, and carcinogenesis the need for stressful chemotherapy treatments could be eliminated altogether. This project is focused on developing novel photosensitive NO donors that can be incorporated into polymeric systems and used in a fiber optic drug delivery system. Development of these NO-releasing polymers will allow continued investigation of NO's role in tumor death by precisely controlling the surface flux of NO that cells are exposed to. Generating specific surface fluxes of NO from polymer films has been demonstrated by using polymer films that contain photoinitiated NO donors [3], prepared by synthesizing S-nitrosothiol (RSNO) derivitized polymer fillers that are blended into hydrophobic polymers and cast into a film. These films generate and sustain a surface flux of NO based on the wavelength and intensity of light used [3]. Polymers releasing NO are more promising as an NO donor than simply injecting NO into samples because they allow for spatial and temporal control of NO delivery. The specific concentration of NO needed to produce desirable effects on tumor cells (i.e., apoptosis) is not known. Data will be presented that show the synthesis and NO-release properties of novel RSNOs based on the nitrosation of benzyl mercaptan thiols. Specifically, UV-Vis spectrum of benzyl mercaptan in toluene and S-nitrosobenzyl mercaptan after the addition of t-butyl nitrite will be presented. We are currently investigating the effects of varying NO-surface fluxes generated from photolytic NO donating polymer films on aortic smooth muscle cell cultures obtained from mice. Once we have established that we can quantitatively determine the effects of different levels of NO on the proliferation of smooth muscle cell cultures, work will begin to apply this methodology and these novel NO-releasing polymeric systems to begin investigating what durations and surface fluxes of NO are necessary to have tumorcidal effects on specific cancer cells.

scholarly journals Development of Photoinitiated Nitric Oxide Releasing Polymer Films for Controlled Drug Delivery

2009 ◽  
Vol 3 (2) ◽  
Author(s):  
D. M. Smeenge ◽  
M. C. Frost
Keyword(s):  
Nitric Oxide ◽  
Drug Delivery ◽  
Smooth Muscle ◽  
Cell Cultures ◽  
Polymer Films ◽  
Surface Flux ◽  
Surface Fluxes ◽  
No Donors ◽  
Polymeric Systems ◽  
Smooth Muscle Cell Cultures

Nitric Oxide (NO) is small, free radical gas that has been shown to have a wide variety of physiological functions, including the ability to hinder tumor angiogenesis at high, but non lethal, concentrations. Previous work suggests that if NO could be effectively delivered in vivo to tumors of patients currently undergoing chemotherapy treatments at the appropriate levels, less damaging chemotherapy treatments could be used against cancer. This could increase the overall survivability of cancer patients, especially in those prone to the harmful effects of chemotherapy: children, elderly, and those of weak immune systems. If NO is especially successful at preventing and eliminating tumor growth, angiogenesis, and carcinogenesis the need for stressful chemotherapy treatments could be eliminated altogether. This project is focused on developing novel photosensitive NO donors that can be incorporated into polymeric systems and used in a fiber optic drug delivery system. Development of these NO-releasing polymers will allow continued investigation of NO's role in tumor death by precisely controlling the surface flux of NO that cells are exposed to. Generating specific surface fluxes of NO from polymer films has been demonstrated by using polymer films that contain photoinitiated NO donors, prepared by synthesizing S-nitrosothiol (RSNO) derivitized polymer fillers that are blended into hydrophobic polymers and cast into a film. These films generate and sustain a surface flux of NO based on the wavelength and intensity of light used. Polymers releasing NO are more promising as an NO donor than simply injecting NO into samples because they allow for spatial and temporal control of NO delivery. The specific concentration of NO needed to produce desirable effects on tumor cells (i.e. apoptosis) is not known. Data will be presented that show the synthesis and NO-release properties of novel RSNOs based on the nitrosation of benzyl mercaptan thiols. Specifically, UV-Vis spectrum of benzyl mercaptan in toluene and S-nitrosobenzyl mercaptan after the addition of t-butyl nitrite will be presented. We are currently investigating the effects of varying NO-surface fluxes generated from photolytic NO donating polymer films on aortic smooth muscle cell cultures obtained from mice. Once we have established that we can quantitatively determine the effects of different levels of NO on the proliferation of smooth muscle cell cultures, work will begin to apply this methodology and these novel NO-releasing polymeric systems to begin investigating what durations and surface fluxes of NO are necessary to have tumorcidal effects on specific cancer cells.

scholarly journals Antibacterial efficacy from NO-releasing MOF–polymer films

2020 ◽  
Vol 1 (7) ◽  
pp. 2509-2519
Author(s):  
Morven J. Duncan ◽  
Paul S. Wheatley ◽  
Emma M. Coghill ◽  
Simon M. Vornholt ◽  
Stewart J. Warrender ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Metal Organic Framework ◽  
Antibacterial Efficacy ◽  
Metal Organic ◽  
Sufficient Concentration ◽  
Organic Framework

Sufficient concentration of nitric oxide is released from metal organic framework loaded polymer films to impart antibacterial efficacy.

scholarly journals Antimicrobial nitric oxide releasing surfaces based on S-nitroso-N-acetylpenicillamine impregnated polymers combined with submicron-textured surface topography

2017 ◽  
Vol 5 (7) ◽  
pp. 1265-1278 ◽  
Author(s):  
Yaqi Wo ◽  
Li-Chong Xu ◽  
Zi Li ◽  
Adam J. Matzger ◽  
Mark E. Meyerhoff ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Surface Topography ◽  
Polymer Films ◽  
Bacterial Adhesion ◽  
Synergistic Effects ◽  
Textured Surface ◽  
No Release ◽  
Nitric Oxide Releasing

SNAP-impregnated textured polymer films having up to 38 day NO-release were shown to have synergistic effects in inhibiting bacterial adhesion.

Plasma-modified nitric oxide-releasing polymer films exhibit time-delayed 8-log reduction in growth of bacteria

2016 ◽  
Vol 11 (3) ◽  
pp. 031005 ◽  
Author(s):  
Michelle N. Mann ◽  
Bella H. Neufeld ◽  
Morgan J. Hawker ◽  
Adoracion Pegalajar-Jurado ◽  
Lindsey N. Paricio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Log Reduction ◽  
Nitric Oxide Releasing ◽  
Growth Of Bacteria

scholarly journals Covalent Linking of pH-Sensitive Dye to Fumed Silica

2010 ◽  
Vol 4 (2) ◽  
Author(s):  
Matthew Nielsen ◽  
Megan C. Frost
Keyword(s):  
Nitric Oxide ◽  
Fumed Silica ◽  
Thrombus Formation ◽  
Sol Gel ◽  
Ph Sensor ◽  
Healthcare Providers ◽  
Blood Gases ◽  
Blood Gas ◽  
Sensor Surface ◽  
Deprotonated Form

Measuring the blood gases of patients in the intensive care unit or undergoing major surgical procedures in real time would help healthcare providers diagnose and manage base excess and base deficient disorders. Optical fibers provide a platform upon which an intravascular blood gas sensor may be built and has been by various companies. Unfortunately, thrombosis on the sensor surface interferes with the blood gas measurements and also poses the risk of creating emboli. Nitric oxide inhibits platelet adhesion and activation, which can reduce thrombus formation on the sensor surface. An optically based pH sensor is fabricated as a first step to show that nitric oxide can be used with blood gas sensors to reduce thrombosis and not interfere with the measurements. pH sensors fabricated using glass microscope slides suffered from leaching of the dye from the sol-gel matrix. The dye readily leached out when the dye was in its appropriate protonated or deprotonated form. To reduce the leaching of the dye, methods of covalently linking the dye to fumed silica have been investigated, and one is presented here.

scholarly journals Nitric Oxide Detection with Glassy Carbon Electrodes Coated with Charge-different Polymer Films

Sensors ◽  
2005 ◽  
Vol 5 (4) ◽  
pp. 161-170 ◽  
Author(s):  
Osamu Ikeda ◽  
Kumiko Yoshinaga ◽  
Jianping Lei
Keyword(s):  
Nitric Oxide ◽  
Glassy Carbon ◽  
Polymer Films ◽  
Carbon Electrodes ◽  
Glassy Carbon Electrodes
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