Spontaneous Catalytic Generation of Nitric Oxide fromS-Nitrosothiols at the Surface of Polymer Films Doped with Lipophilic Copper(II) Complex

2003 ◽  
Vol 125 (32) ◽  
pp. 9552-9553 ◽  
Author(s):  
Bong Kyun Oh ◽  
Mark E. Meyerhoff
Keyword(s):  
Nitric Oxide ◽  
Polymer Films

scholarly journals Development of Photoinitiated Nitric Oxide Releasing Polymer Films for Controlled Drug Delivery

2009 ◽  
Vol 3 (2) ◽  
Author(s):  
D. M. Smeenge ◽  
M. J. Barron ◽  
M. T. Nielsen ◽  
J. Goldman ◽  
M. C. Frost
Keyword(s):  
Nitric Oxide ◽  
Drug Delivery ◽  
Smooth Muscle ◽  
Cell Cultures ◽  
Polymer Films ◽  
Surface Flux ◽  
Surface Fluxes ◽  
No Donors ◽  
Polymeric Systems ◽  
Smooth Muscle Cell Cultures

Nitric Oxide (NO) is small, free radical gas that has been shown to have a wide variety of physiological functions, including the ability to hinder tumor angiogenesis at high, but non lethal, concentrations [1]. Previous work suggests that if NO could be effectively delivered in vivo to tumors of patients currently undergoing chemotherapy treatments at the appropriate levels, less damaging chemotherapy treatments could be used against cancer [2]. This could increase the overall survivability of cancer patients, especially in those prone to the harmful effects of chemotherapy: children, elderly, and those of weak immune systems. If NO is especially successful at preventing and eliminating tumor growth, angiogenesis, and carcinogenesis the need for stressful chemotherapy treatments could be eliminated altogether. This project is focused on developing novel photosensitive NO donors that can be incorporated into polymeric systems and used in a fiber optic drug delivery system. Development of these NO-releasing polymers will allow continued investigation of NO's role in tumor death by precisely controlling the surface flux of NO that cells are exposed to. Generating specific surface fluxes of NO from polymer films has been demonstrated by using polymer films that contain photoinitiated NO donors [3], prepared by synthesizing S-nitrosothiol (RSNO) derivitized polymer fillers that are blended into hydrophobic polymers and cast into a film. These films generate and sustain a surface flux of NO based on the wavelength and intensity of light used [3]. Polymers releasing NO are more promising as an NO donor than simply injecting NO into samples because they allow for spatial and temporal control of NO delivery. The specific concentration of NO needed to produce desirable effects on tumor cells (i.e., apoptosis) is not known. Data will be presented that show the synthesis and NO-release properties of novel RSNOs based on the nitrosation of benzyl mercaptan thiols. Specifically, UV-Vis spectrum of benzyl mercaptan in toluene and S-nitrosobenzyl mercaptan after the addition of t-butyl nitrite will be presented. We are currently investigating the effects of varying NO-surface fluxes generated from photolytic NO donating polymer films on aortic smooth muscle cell cultures obtained from mice. Once we have established that we can quantitatively determine the effects of different levels of NO on the proliferation of smooth muscle cell cultures, work will begin to apply this methodology and these novel NO-releasing polymeric systems to begin investigating what durations and surface fluxes of NO are necessary to have tumorcidal effects on specific cancer cells.

scholarly journals Development of Photoinitiated Nitric Oxide Releasing Polymer Films for Controlled Drug Delivery

2009 ◽  
Vol 3 (2) ◽  
Author(s):  
D. M. Smeenge ◽  
M. C. Frost
Keyword(s):  
Nitric Oxide ◽  
Drug Delivery ◽  
Smooth Muscle ◽  
Cell Cultures ◽  
Polymer Films ◽  
Surface Flux ◽  
Surface Fluxes ◽  
No Donors ◽  
Polymeric Systems ◽  
Smooth Muscle Cell Cultures

Nitric Oxide (NO) is small, free radical gas that has been shown to have a wide variety of physiological functions, including the ability to hinder tumor angiogenesis at high, but non lethal, concentrations. Previous work suggests that if NO could be effectively delivered in vivo to tumors of patients currently undergoing chemotherapy treatments at the appropriate levels, less damaging chemotherapy treatments could be used against cancer. This could increase the overall survivability of cancer patients, especially in those prone to the harmful effects of chemotherapy: children, elderly, and those of weak immune systems. If NO is especially successful at preventing and eliminating tumor growth, angiogenesis, and carcinogenesis the need for stressful chemotherapy treatments could be eliminated altogether. This project is focused on developing novel photosensitive NO donors that can be incorporated into polymeric systems and used in a fiber optic drug delivery system. Development of these NO-releasing polymers will allow continued investigation of NO's role in tumor death by precisely controlling the surface flux of NO that cells are exposed to. Generating specific surface fluxes of NO from polymer films has been demonstrated by using polymer films that contain photoinitiated NO donors, prepared by synthesizing S-nitrosothiol (RSNO) derivitized polymer fillers that are blended into hydrophobic polymers and cast into a film. These films generate and sustain a surface flux of NO based on the wavelength and intensity of light used. Polymers releasing NO are more promising as an NO donor than simply injecting NO into samples because they allow for spatial and temporal control of NO delivery. The specific concentration of NO needed to produce desirable effects on tumor cells (i.e. apoptosis) is not known. Data will be presented that show the synthesis and NO-release properties of novel RSNOs based on the nitrosation of benzyl mercaptan thiols. Specifically, UV-Vis spectrum of benzyl mercaptan in toluene and S-nitrosobenzyl mercaptan after the addition of t-butyl nitrite will be presented. We are currently investigating the effects of varying NO-surface fluxes generated from photolytic NO donating polymer films on aortic smooth muscle cell cultures obtained from mice. Once we have established that we can quantitatively determine the effects of different levels of NO on the proliferation of smooth muscle cell cultures, work will begin to apply this methodology and these novel NO-releasing polymeric systems to begin investigating what durations and surface fluxes of NO are necessary to have tumorcidal effects on specific cancer cells.

scholarly journals Antibacterial efficacy from NO-releasing MOF–polymer films

2020 ◽  
Vol 1 (7) ◽  
pp. 2509-2519
Author(s):  
Morven J. Duncan ◽  
Paul S. Wheatley ◽  
Emma M. Coghill ◽  
Simon M. Vornholt ◽  
Stewart J. Warrender ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Metal Organic Framework ◽  
Antibacterial Efficacy ◽  
Metal Organic ◽  
Sufficient Concentration ◽  
Organic Framework

Sufficient concentration of nitric oxide is released from metal organic framework loaded polymer films to impart antibacterial efficacy.

scholarly journals Antimicrobial nitric oxide releasing surfaces based on S-nitroso-N-acetylpenicillamine impregnated polymers combined with submicron-textured surface topography

2017 ◽  
Vol 5 (7) ◽  
pp. 1265-1278 ◽  
Author(s):  
Yaqi Wo ◽  
Li-Chong Xu ◽  
Zi Li ◽  
Adam J. Matzger ◽  
Mark E. Meyerhoff ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Surface Topography ◽  
Polymer Films ◽  
Bacterial Adhesion ◽  
Synergistic Effects ◽  
Textured Surface ◽  
No Release ◽  
Nitric Oxide Releasing

SNAP-impregnated textured polymer films having up to 38 day NO-release were shown to have synergistic effects in inhibiting bacterial adhesion.

Plasma-modified nitric oxide-releasing polymer films exhibit time-delayed 8-log reduction in growth of bacteria

2016 ◽  
Vol 11 (3) ◽  
pp. 031005 ◽  
Author(s):  
Michelle N. Mann ◽  
Bella H. Neufeld ◽  
Morgan J. Hawker ◽  
Adoracion Pegalajar-Jurado ◽  
Lindsey N. Paricio ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Log Reduction ◽  
Nitric Oxide Releasing ◽  
Growth Of Bacteria

scholarly journals Nitric Oxide Detection with Glassy Carbon Electrodes Coated with Charge-different Polymer Films

Sensors ◽  
2005 ◽  
Vol 5 (4) ◽  
pp. 161-170 ◽  
Author(s):  
Osamu Ikeda ◽  
Kumiko Yoshinaga ◽  
Jianping Lei
Keyword(s):  
Nitric Oxide ◽  
Glassy Carbon ◽  
Polymer Films ◽  
Carbon Electrodes ◽  
Glassy Carbon Electrodes

Correlating S-nitrosothiol decomposition and NO release for modified poly(lactic-co-glycolic acid) polymer films

RSC Advances ◽  
2014 ◽  
Vol 4 (79) ◽  
pp. 42039-42043 ◽  
Author(s):  
J. M. Joslin ◽  
B. H. Neufeld ◽  
Melissa M. Reynolds
Keyword(s):  
Nitric Oxide ◽  
Polymer Films ◽  
Glycolic Acid ◽  
No Release

The decomposition of an S-nitrosated model polymer was correlated to the subsequent release of nitric oxide under multiple decomposition pathways.

Gum arabic helps prepare better lacey polymer films for specimen support in electron microscopy

1993 ◽  
Vol 51 ◽  
pp. 240-241
Author(s):  
Shailesh R. Sheth ◽  
Jayesh R. Bellare
Keyword(s):  
Electron Microscopy ◽  
Polymer Films ◽  
Gum Arabic ◽  
Complete Removal ◽  
Ammonium Bromide ◽  
Release Agent ◽  
Astigmatism Correction ◽  
Long Time ◽  
Micro Holes ◽  
Cetyl Trimethyl Ammonium

Specimen support and astigmatism correction in Electron Microscopy are at least two areas in which lacey polymer films find extensive applications. Although their preparation has been studied for a very long time, present techniques still suffer from incomplete release of the film from its substrate and presence of a large number of pseudo holes in the film. Our method ensures complete removal of the entire lacey film from the substrate and fewer pseudo holes by pre-treating the substrate with Gum Arabic, which acts as a film release agent.The method is based on the classical condensation technique for preparing lacey films which is essentially deposition of minute water or ice droplets on the substrate and laying the polymer film over it, so that micro holes are formed corresponding to the droplets. A microscope glass slide (the substrate) is immersed in 2.0% (w/v) aq. CTAB (cetyl trimethyl ammonium bromide)-0.22% (w/v) aq.

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