High-resolution two-dimensional in-phase multiplets in nuclear magnetic resonance correlation spectroscopy

1992 ◽  
Vol 114 (27) ◽  
pp. 10989-10990 ◽  
Author(s):  
Sebastien J. F. Vincent ◽  
Catherine Zwahlen ◽  
Geoffrey Bodenhausen
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
Nuclear Magnetic

The structure of the antigenic lipopolysaccharide O-chain produced by Escherichiahermannii ATCC 33650 and 33652

1990 ◽  
Vol 68 (8) ◽  
pp. 1456-1466 ◽  
Author(s):  
Linda M. Beynon ◽  
David R. Bundle ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Molecular Modelling ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Structure Formula ◽  
Structural Methods ◽  
Nuclear Magnetic

High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.

Quantitative 13C N.M.R. Assay and Assignment of Mixtures from Lipase Digestion of Propane-1,2-diol Dibutyrate

1999 ◽  
Vol 52 (2) ◽  
pp. 123 ◽  
Author(s):  
Charmian J. O'Connor ◽  
Richard H. Barton
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Polarization Transfer ◽  
Correlation Spectroscopy ◽  
Two Dimensional ◽  
13C Nuclear Magnetic Resonance ◽  
Inductive Effects ◽  
Nuclear Magnetic

13C nuclear magnetic resonance (n.m.r.) spectra were obtained at 50 and 100 MHz for mixtures of propanediol, propane-1,2-diol dibutyrate, propane-1,2-diol 1-butyrate and propane-1,2-diol 2-butyrate in CDCl3. Distortionless enhancement by polarization transfer (DEPT) and two-dimensional 13C–1H correlation spectroscopy were used to confirm shift assignments. Spectra for the 1- and 2-monoesters showed strongly different inductive effects due to the position of the butyryl chain. These experiments demonstrate the desirability of using 13C n.m.r. rather than 1H n.m.r. spectroscopy in quantitative speciation of acylated diol complex hydrolysis mixtures.

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