Internal energy state distribution of imidogen(A3.PI.) from the chemiluminescent reaction of methylidyne(X2.PI.,v" = 0) with nitric oxide

1986 ◽  
Vol 90 (8) ◽  
pp. 1491-1493 ◽  
Author(s):  
Nobuaki Nishiyama ◽  
Hiroshi Sekiya ◽  
Sumio Yamaguchi ◽  
Masaharu Tsuji ◽  
Yukio Nishimura
Keyword(s):  
Nitric Oxide ◽  
Internal Energy ◽  
Energy State ◽  
State Distribution ◽  
Chemiluminescent Reaction

scholarly journals Heme dynamics and trafficking factors revealed by genetically encoded fluorescent heme sensors

2016 ◽  
Vol 113 (27) ◽  
pp. 7539-7544 ◽  
Author(s):  
David A. Hanna ◽  
Raven M. Harvey ◽  
Osiris Martinez-Guzman ◽  
Xiaojing Yuan ◽  
Bindu Chandrasekharan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Glycolytic Enzyme ◽  
Unicellular Eukaryote ◽  
Human Cell Lines ◽  
State Distribution ◽  
Signaling Molecule ◽  
Nuclear Transcription Factor ◽  
Heme Acquisition ◽  
Oxidation State Distribution ◽  
Heme Trafficking

Heme is an essential cofactor and signaling molecule. Heme acquisition by proteins and heme signaling are ultimately reliant on the ability to mobilize labile heme (LH). However, the properties of LH pools, including concentration, oxidation state, distribution, speciation, and dynamics, are poorly understood. Herein, we elucidate the nature and dynamics of LH using genetically encoded ratiometric fluorescent heme sensors in the unicellular eukaryoteSaccharomyces cerevisiae. We find that the subcellular distribution of LH is heterogeneous; the cytosol maintains LH at ∼20–40 nM, whereas the mitochondria and nucleus maintain it at concentrations below 2.5 nM. Further, we find that the signaling molecule nitric oxide can initiate the rapid mobilization of heme in the cytosol and nucleus from certain thiol-containing factors. We also find that the glycolytic enzyme glyceraldehyde phosphate dehydrogenase constitutes a major cellular heme buffer, and is responsible for maintaining the activity of the heme-dependent nuclear transcription factor heme activator protein (Hap1p). Altogether, we demonstrate that the heme sensors can be used to reveal fundamental aspects of heme trafficking and dynamics and can be used across multiple organisms, includingEscherichia coli, yeast, and human cell lines.

Kinetics of the chemiluminescent reaction between nitric oxide and ozone

1964 ◽  
Vol 60 ◽  
pp. 359 ◽  
Author(s):  
M. A. A. Clyne ◽  
B. A. Thrush ◽  
R. P. Wayne
Keyword(s):  
Nitric Oxide ◽  
Chemiluminescent Reaction ◽  
Kinetics Of

Non-LTE state distribution of nitric oxide and its impact on the retrieval of the stratospheric daytime no profile from MIPAS limb sounding instruments

2000 ◽  
Vol 26 (6) ◽  
pp. 947-950 ◽  
Author(s):  
B. Funke ◽  
M. López-Puertas ◽  
G. Stiller ◽  
T.v. Clarmann ◽  
M. Höpfner ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
State Distribution

scholarly journals Nitric oxide production is involved in maintaining energy state in Alfalfa (Medicago sativa L.) nodulated roots under both salinity and flooding

Planta ◽  
2020 ◽  
Vol 252 (2) ◽  
Author(s):  
Fatma Aridhi ◽  
Hajer Sghaier ◽  
Allyzée Gaitanaros ◽  
Ayda Khadri ◽  
Samira Aschi-Smiti ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Medicago Sativa ◽  
Energy State ◽  
Nitric Oxide Production ◽  
Oxide Production ◽  
Medicago Sativa L

scholarly journals A Rapid and Selective Method for Determining Potential Nitrosating Agents in Cosmetic Products by Chemiluminescence Detection of Nitric Oxide

1998 ◽  
Vol 81 (2) ◽  
pp. 368-372 ◽  
Author(s):  
Hardy J Chou ◽  
Ronald L Yates
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
False Positive ◽  
Nitroso Compounds ◽  
Cosmetic Products ◽  
Chemiluminescence Detection ◽  
Selective Method ◽  
Selective Determination ◽  
Chemiluminescent Reaction

abstract A method was developed for rapid and selective determination of potential nitrosating agents at the part-per-billion level in cosmetic products. These compounds are chemically reduced to nitric oxide, which is determined by its chemiluminescent reaction with ozone. Suspended materials and colors in cosmetic products do not interfere. Hence their removal before analysis is not required. A detection limit of 33 ppb, calculated as nitrite, was obtained. No false-positive interferences were observed from antifoaming agents, several AZ-nitroso compounds, and nitrate up to 20 ppm. Among cosmetic products surveyed, potential nitrosating agents were found at levels ranging from 113 to 5021 ppb. No consistent relationship was found between levels of potential nitrosating agents and N-nitrosamines in the same products. However, the highest levels of nitrosating agents were most often associated with the highest levels of N-nitrosamines known to be present in the products.

Abstract 1232: Regulation of Mitochondria membrane Potential by Shear Stress: Implication of NO Signaling

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Mahsa Rouhanizadeh ◽  
Ryan Hamilton ◽  
Enrique Cadenas ◽  
Tzung Hsiai
Keyword(s):  
Nitric Oxide ◽  
Shear Stress ◽  
Membrane Potential ◽  
Manganese Superoxide Dismutase ◽  
Fluorescent Dyes ◽  
Energy State ◽  
No Production ◽  
List Type ◽  
No Donor ◽  
Mitochondria Membrane Potential

Introduction : Mitochondria are the most important source of intracellular reactive oxygen species (ROS) in vascular endothelial cells (EC). Mitochondrial membrane potential (ΔΨm) is an indicator of mitochondrial energy state and mtROS production. Shear stress is known to induce eNOS and produce nitric oxide (NO). Hypothesis: We assessed whether NO is implicated in shear stress-induced ΔΨm and mtROS generation. Methods : Confluent HAEC were subjected to three conditions: static control (τ ave = 0 dyn.cm −2 at ∂τ/∂t = 0); pulsatile shear stress (PSS) at a mean shear stress (τ ave ) of 23 dyn.cm −2 with a temporal variation (∂τ/∂t) at 71 dyn.cm −2 .s −1 ; and oscillatory shear stress (OSS) at τ ave = 0.02 with ∂τ/∂t at ±3 dyn.cm − 2.s −1 . Tetramethyl rhodamine methyl ester (TMRM) and Mitosox red fluorescent dyes were used to assess mitochondrial ΔΨm and O 2 ·− production respectively. Intracellular H 2 O 2 production was assessed by carboxy-H 2 DCFDA. HAEC were treated with DETA-NO (NO donor) and L-NAME (eNOS inhibitor) to assess how shear stress-induced NO production regulates ΔΨm. Results: Delta;Ψm was increased by 12±5.3 mV in response to OSS, and by 33.5±8.9 mV in response to PSS (n=3, P <0.05) relative to static control. DETA-NO at 0.21mM and 0.94mM was used to simulate PSS-induced NO production during 1 hour and 4 hours of flow and it increased Delta;Ψm by 9.6±4.9 mV and 18.9 ±3.2 mV, respectively (n=3, P<0.05). L-NAME reduced Delta;Ψm (n=3, P<0.05) suggesting that NO is involved in Delta;Ψm regulation. PSS-induced Delta;Ψm was accompanied by a 1.4 fold increase in mtROS production (n=3, P<0.05) and a concomitant 10 fold upregulation in mitochondrial manganese superoxide dismutase (MnSOD) mRNA expression (n=6, P<0.05). Conclusion: Nitric oxide modulates Delta;Ψm and mtROS production. PSS, which upregulates eNOS, is more potent than OSS at increasing Delta;Ψm and mtROS, implicating NO as a signaling molecule in mitochondrial and endothelial function.

NO +O Chemiluminescent Reaction Using Adiabatically Expanded Nitric Oxide

1967 ◽  
Vol 46 (8) ◽  
pp. 3275-3276 ◽  
Author(s):  
Arthur Fontijn ◽  
Daniel E. Rosner
Keyword(s):  
Nitric Oxide ◽  
Chemiluminescent Reaction
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