Modeling Removal of Cd, Cu, Pb, and Zn in Acidic Groundwater during Neutralization by Ambient Surface Waters and Groundwaters

1999 ◽  
Vol 33 (21) ◽  
pp. 3850-3856 ◽  
Author(s):  
Anthony J. Paulson ◽  
Laurie Balistrieri
2014 ◽  
Vol 80 (10) ◽  
pp. 3086-3094 ◽  
Author(s):  
Hyatt C. Green ◽  
Richard A. Haugland ◽  
Manju Varma ◽  
Hana T. Millen ◽  
Mark A. Borchardt ◽  
...  

ABSTRACTQuantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genusBacteroidesare among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.


2020 ◽  
Vol 176 ◽  
pp. 115729 ◽  
Author(s):  
Gary S. Russo ◽  
Sorina E. Eftim ◽  
Alexandra E. Goldstone ◽  
Alfred P. Dufour ◽  
Sharon P. Nappier ◽  
...  

2010 ◽  
Vol 44 (13) ◽  
pp. 5049-5054 ◽  
Author(s):  
Richard L. Whitman ◽  
Zhongfu Ge ◽  
Meredith B. Nevers ◽  
Alexandria B. Boehm ◽  
Eunice C. Chern ◽  
...  

Author(s):  
Amanda. L. Jarvis ◽  
James R. Justice ◽  
Michael C. Elias ◽  
Brian Schnitker ◽  
Kathryn Gallagher

1985 ◽  
Vol 6 (2) ◽  
pp. 52-58 ◽  
Author(s):  
Susan T. Bagley

AbstractThe genus Klebsiella is seemingly ubiquitous in terms of its habitat associations. Klebsiella is a common opportunistic pathogen for humans and other animals, as well as being resident or transient flora (particularly in the gastrointestinal tract). Other habitats include sewage, drinking water, soils, surface waters, industrial effluents, and vegetation. Until recently, almost all these Klebsiella have been identified as one species, ie, K. pneumoniae. However, phenotypic and genotypic studies have shown that “K. pneumoniae” actually consists of at least four species, all with distinct characteristics and habitats. General habitat associations of Klebsiella species are as follows: K. pneumoniae—humans, animals, sewage, and polluted waters and soils; K. oxytoca—frequent association with most habitats; K. terrigena— unpolluted surface waters and soils, drinking water, and vegetation; K. planticola—sewage, polluted surface waters, soils, and vegetation; and K. ozaenae/K. rhinoscleromatis—infrequently detected (primarily with humans).


Author(s):  
James S. Webber

INTRODUCTION“Acid rain” and “acid deposition” are terms no longer confined to the lexicon of atmospheric scientists and 1imnologists. Public awareness of and concern over this phenomenon, particularly as it affects acid-sensitive regions of North America, have increased dramatically in the last five years. Temperate ecosystems are suffering from decreased pH caused by acid deposition. Human health may be directly affected by respirable sulfates and by the increased solubility of toxic trace metals in acidified waters. Even man's monuments are deteriorating as airborne acids etch metal and stone features.Sulfates account for about two thirds of airborne acids with wet and dry deposition contributing equally to acids reaching surface waters or ground. The industrial Midwest is widely assumed to be the source of most sulfates reaching the acid-sensitive Northeast since S02 emitted as a byproduct of coal combustion in the Midwest dwarfs S02 emitted from all sources in the Northeast.


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