Relationship and Variation of qPCR and Culturable Enterococci Estimates in Ambient Surface Waters Are Predictable

2010 ◽  
Vol 44 (13) ◽  
pp. 5049-5054 ◽  
Author(s):  
Richard L. Whitman ◽  
Zhongfu Ge ◽  
Meredith B. Nevers ◽  
Alexandria B. Boehm ◽  
Eunice C. Chern ◽  
...  
Keyword(s):  
Surface Waters ◽  
Ambient Surface

scholarly journals Improved HF183 Quantitative Real-Time PCR Assay for Characterization of Human Fecal Pollution in Ambient Surface Water Samples

2014 ◽  
Vol 80 (10) ◽  
pp. 3086-3094 ◽  
Author(s):  
Hyatt C. Green ◽  
Richard A. Haugland ◽  
Manju Varma ◽  
Hana T. Millen ◽  
Mark A. Borchardt ◽  
...  
Keyword(s):  
Real Time ◽  
Surface Waters ◽  
Real Time Pcr ◽  
Fecal Pollution ◽  
Qpcr Assay ◽  
Taqman Assay ◽  
Reference Database ◽  
Quantitative Real Time Pcr ◽  
Ambient Surface

ABSTRACTQuantitative real-time PCR (qPCR) assays that target the human-associated HF183 bacterial cluster within members of the genusBacteroidesare among the most widely used methods for the characterization of human fecal pollution in ambient surface waters. In this study, we show that a current TaqMan HF183 qPCR assay (HF183/BFDrev) routinely forms nonspecific amplification products and introduce a modified TaqMan assay (HF183/BacR287) that alleviates this problem. The performance of each qPCR assay was compared in head-to-head experiments investigating limits of detection, analytical precision, predicted hybridization to 16S rRNA gene sequences from a reference database, and relative marker concentrations in fecal and sewage samples. The performance of the modified HF183/BacR287 assay is equal to or improves upon that of the original HF183/BFDrev assay. In addition, a qPCR chemistry designed to combat amplification inhibition and a multiplexed internal amplification control are included. In light of the expanding use of PCR-based methods that rely on the detection of extremely low concentrations of DNA template, such as qPCR and digital PCR, the new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.

scholarly journals Evaluating health risks associated with exposure to ambient surface waters during recreational activities: A systematic review and meta-analysis

2020 ◽  
Vol 176 ◽  
pp. 115729 ◽  
Author(s):  
Gary S. Russo ◽  
Sorina E. Eftim ◽  
Alexandra E. Goldstone ◽  
Alfred P. Dufour ◽  
Sharon P. Nappier ◽  
...  
Keyword(s):  
Systematic Review ◽  
Health Risks ◽  
Surface Waters ◽  
Meta Analysis ◽  
Recreational Activities ◽  
Ambient Surface

scholarly journals PFOS in U.S. Ambient Surface Waters: A Review of Occurrence in Aquatic Environments and Comparison to Global Concentrations

2021 ◽  
Author(s):  
Amanda. L. Jarvis ◽  
James R. Justice ◽  
Michael C. Elias ◽  
Brian Schnitker ◽  
Kathryn Gallagher
Keyword(s):  
Surface Waters ◽  
Aquatic Environments ◽  
Ambient Surface

Habitat Association of Klebsiella Species

1985 ◽  
Vol 6 (2) ◽  
pp. 52-58 ◽  
Author(s):  
Susan T. Bagley
Keyword(s):  
Drinking Water ◽  
Gastrointestinal Tract ◽  
Surface Waters ◽  
Industrial Effluents ◽  
Opportunistic Pathogen ◽  
Habitat Associations ◽  
Habitat Association ◽  
Klebsiella Species ◽  
Almost All ◽  
Polluted Waters

AbstractThe genus Klebsiella is seemingly ubiquitous in terms of its habitat associations. Klebsiella is a common opportunistic pathogen for humans and other animals, as well as being resident or transient flora (particularly in the gastrointestinal tract). Other habitats include sewage, drinking water, soils, surface waters, industrial effluents, and vegetation. Until recently, almost all these Klebsiella have been identified as one species, ie, K. pneumoniae. However, phenotypic and genotypic studies have shown that “K. pneumoniae” actually consists of at least four species, all with distinct characteristics and habitats. General habitat associations of Klebsiella species are as follows: K. pneumoniae—humans, animals, sewage, and polluted waters and soils; K. oxytoca—frequent association with most habitats; K. terrigena— unpolluted surface waters and soils, drinking water, and vegetation; K. planticola—sewage, polluted surface waters, soils, and vegetation; and K. ozaenae/K. rhinoscleromatis—infrequently detected (primarily with humans).

Identifying Sources of Sulfate Aerosols Reaching Whiteface Mountain, NY

1985 ◽  
Vol 43 ◽  
pp. 98-101
Author(s):  
James S. Webber
Keyword(s):  
Trace Metals ◽  
Acid Deposition ◽  
Surface Waters ◽  
Dry Deposition ◽  
Coal Combustion ◽  
Public Awareness ◽  
Sulfate Aerosols ◽  
Wet And Dry Deposition ◽  
Whiteface Mountain ◽  
Toxic Trace Metals

INTRODUCTION“Acid rain” and “acid deposition” are terms no longer confined to the lexicon of atmospheric scientists and 1imnologists. Public awareness of and concern over this phenomenon, particularly as it affects acid-sensitive regions of North America, have increased dramatically in the last five years. Temperate ecosystems are suffering from decreased pH caused by acid deposition. Human health may be directly affected by respirable sulfates and by the increased solubility of toxic trace metals in acidified waters. Even man's monuments are deteriorating as airborne acids etch metal and stone features.Sulfates account for about two thirds of airborne acids with wet and dry deposition contributing equally to acids reaching surface waters or ground. The industrial Midwest is widely assumed to be the source of most sulfates reaching the acid-sensitive Northeast since S02 emitted as a byproduct of coal combustion in the Midwest dwarfs S02 emitted from all sources in the Northeast.

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