Development of an inhibition Magnetic Enzyme ImmunoAssay (MEIA) technique for determination of staphylococcal enterotoxin B

1984 ◽  
Vol 12 (2) ◽  
pp. 264-265 ◽  
Author(s):  
PRADIP D. PATEL ◽  
PAUL A. GIBBS
Keyword(s):  
Enzyme Immunoassay ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

An ultrasensitive immunosensor array for determination of staphylococcal enterotoxin B

Talanta ◽  
2011 ◽  
Vol 85 (2) ◽  
pp. 1070-1074 ◽  
Author(s):  
Xiaoming Zhang ◽  
Fei Liu ◽  
Ruifang Yan ◽  
Pan Xue ◽  
Yongming Li ◽  
...  
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Enterotoxin B

Triple-Mode Aptasensor for Sensitive and Reliable Determination of Staphylococcal Enterotoxin B

2020 ◽  
Vol 13 (6) ◽  
pp. 1255-1261
Author(s):  
Zhengzong Wu ◽  
Deyun He ◽  
Bo Cui ◽  
Zhengyu Jin ◽  
Enbo Xu
Keyword(s):  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Reliable Determination ◽  
Enterotoxin B ◽  
Triple Mode

Colorimetric determination of staphylococcal enterotoxin B via DNAzyme-guided growth of gold nanoparticles

2016 ◽  
Vol 183 (10) ◽  
pp. 2753-2760 ◽  
Author(s):  
Dandan Zhou ◽  
Guoming Xie ◽  
Xianqing Cao ◽  
Xueping Chen ◽  
Xing Zhang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Colorimetric Determination ◽  
Guided Growth ◽  
Enterotoxin B

Simple and Rapid Inhibition Enzyme Immunoassay for the Detection of Staphylococcal Enterotoxin B in Foods

1990 ◽  
Vol 53 (10) ◽  
pp. 834-840 ◽  
Author(s):  
CÉLINE MORISSETTE ◽  
JACQUES GOULET ◽  
GILLES LAMOUREUX
Keyword(s):  
Enzymatic Activity ◽  
Enzyme Immunoassay ◽  
Protein A ◽  
Staphylococcal Enterotoxin ◽  
Staphylococcal Enterotoxin B ◽  
Minimal Level ◽  
Inhibition Elisa ◽  
Promising Tool ◽  
Enterotoxin B ◽  
Peroxidase Conjugate

An absorption-inhibition ELISA (AI-ELISA) was developed to detect, within an hour, the staphylococcal enterotoxin B (SEB) in solutions and food homogenates. The SEB-containing sample is first incubated with a standardized concentration of antibody. The quantity of nonabsorbed anti-SEB Ab is evaluated by incubating a SEB-coated dipstick in the reaction mixture. The protein A-peroxidase conjugate is then used to detect the anti-SEB Ab bound to SEB-coated dipsticks. The enzymatic activity developed in a solution containing the substrate and a chromogen is inversely related to the SEB concentration. Concentrations as low as 0.01 to 1 μg SEB/ml can be consistently detected within 20 min. In unconcentrated food homogenates, 100 ng of SEB per ml could be detected by this AI-ELISA. The minimal level of sensitivity could be lowered to at least 1 ng SEB/g of food. The rapidity of the AI-ELISA, its simplicity, and repeatability make this immunoassay a promising tool for routine detections of staphylococcal enterotoxins in foods.

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