Effect of Inhibitors Released during Steam-Explosion Treatment of Poplar Wood on Subsequent Enzymatic Hydrolysis and SSF

2008 ◽  
Vol 20 (1) ◽  
pp. 200-206 ◽  
Author(s):  
Maria Cantarella ◽  
Laura Cantarella ◽  
Alberto Gallifuoco ◽  
Agata Spera ◽  
Francesco Alfani
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Poplar Wood ◽  
Effect Of Inhibitors

Effect of Inhibitors Released During Steam-Explosion Pretreatment of Barley Straw on Enzymatic Hydrolysis

2006 ◽  
Vol 129 (1-3) ◽  
pp. 278-288 ◽  
Author(s):  
MA Prado GArcía-Aparicio ◽  
Ignacio Ballesteros ◽  
Alberto González ◽  
JoséMiguel Oliva ◽  
Mercedes Ballesteros ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Barley Straw ◽  
Steam Explosion Pretreatment ◽  
Effect Of Inhibitors

Effect of Inhibitors Released During Steam-Explosion Pretreatment of Barley Straw on Enzymatic Hydrolysis

2007 ◽  
pp. 278-288 ◽  
Author(s):  
MA Prado García-Aparicio ◽  
Ignacio Ballesteros ◽  
Alberto González ◽  
José Miguel Oliva ◽  
Mercedes Ballesteros ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Barley Straw ◽  
Steam Explosion Pretreatment ◽  
Effect Of Inhibitors

scholarly journals ENZYMATIC HYDROLYSIS OF FAST-GROWING POPLAR WOOD AFTER PRETREATMENT BY STEAM EXPLOSION

2021 ◽  
Vol 55 (5-6) ◽  
pp. 637-647
Author(s):  
ANNA GAŁĄZKA ◽  
JAN SZADKOWSKI
Keyword(s):  
Energy Source ◽  
Chemical Composition ◽  
Enzymatic Hydrolysis ◽  
Natural Gas ◽  
Steam Explosion ◽  
Hard Coal ◽  
Poplar Wood ◽  
Wood Biomass ◽  
Hydrolysis Of ◽  
Heating Medium

The aim of the present study was to investigate the effect of steam explosion pretreatment, without maintaining the heating temperature, on the yield of enzymatic hydrolysis of wood biomass. Genetically modified poplar wood was used for the investigation. The pretreatment process was conducted at temperatures of 160 °C, 175 °C, 190 °C and 205 °C. Then, the system was rapidly decompressed. The heating medium was water. The chemical composition of biomass was determined before and after the steam explosion and then enzymatic hydrolysis was performed. The results of the chemical composition analysis showed a change in the holocellulose content in the analyzed biomass (about 80% for the native sample and 72% for the biomass sample treated at 205 °C), a decrease in the hemicelluloses content from about 40% (native sample) to 16% for the sample treated at 205 °C. The results of enzymatic hydrolysis showed the lowest glucose extraction efficiency for biomass hydrolysis after the treatment at 160 °C, of only about 9% compared to the theoretical content of glucose from the cellulose contained in hydrolysed wood biomass. The highest results were obtained for the samples treated at 190 °C and 205 °C. The study also estimated the processing costs, as a function of the heating medium (steam, water) and energy source (atomic energy, hard coal, natural gas, biomass), assuming heating with electric heaters. From the economic point of view, it is advantageous to use steam heating medium, and either natural gas or biomass as an energy source.

scholarly journals Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 507
Author(s):  
Eduardo Troncoso-Ortega ◽  
Rosario del P. Castillo ◽  
Pablo Reyes-Contreras ◽  
Patricia Castaño-Rivera ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Eucalyptus Globulus ◽  
Steam Explosion ◽  
Laser Scanning ◽  
Structural Changes ◽  
Micro Particles ◽  
Cellulose Accessibility ◽  
Ft Ir ◽  
Enzymatic Accessibility ◽  
Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

Optimization of steam pretreatment conditions for enzymatic hydrolysis of poplar wood

Holzforschung ◽  
2011 ◽  
Vol 65 (4) ◽  
Author(s):  
Fokko Schütt ◽  
Jürgen Puls ◽  
Bodo Saake
Keyword(s):  
Enzymatic Hydrolysis ◽  
Raw Material ◽  
Steam Pretreatment ◽  
Alkaline Extraction ◽  
Poplar Wood ◽  
Steam Refining ◽  
Severity Factor ◽  
Short Rotation ◽  
Pretreatment Conditions ◽  
Hydrolysis Of

Abstract Steam refining was investigated as a pretreatment for enzymatic hydrolysis of poplar wood from a short rotation plantation. The experiments were carried out without debarking to use an economically realistic raw material. Steam refining conditions were varied in the range of 3–30 min and 170–220°C, according to a factorial design created with the software JMP from SAS Institute Inc., Cary, NC, USA. Predicted steaming conditions for highest glucose and xylose yields after enzymatic hydrolysis were at 210°C and 15 min. Control tests under the optimized conditions verified the predicted results. Further pretreatments without bark showed that the enzymes were not significantly inhibited by the bark. The yield of glucose and xylose was 61.9% of theoretical for the experiments with the whole raw material, whereas the yield for the experiments without bark was 63.6%. Alkaline extraction of lignin from the fibers before enzymatic hydrolysis resulted in an increase of glucose yields from mild pretreated fibers and a decrease for severe pretreated fibers. The extracted lignin had a high content of xylose of up to 14% after very mild pretreatments. On the other hand, molecular weights of the extracted lignin increased substantially after pretreatments with a severity factor above 4. Hence, alkaline extraction of the lignin seems only attractive in a narrow range of steaming conditions.

Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline peroxide delignification

2006 ◽  
Vol 41 (2) ◽  
pp. 423-429 ◽  
Author(s):  
Cristóbal Cara ◽  
Encarnación Ruiz ◽  
Ignacio Ballesteros ◽  
María J. Negro ◽  
Eulogio Castro
Keyword(s):  
Enzymatic Hydrolysis ◽  
Steam Explosion ◽  
Olive Tree ◽  
Tree Wood ◽  
Alkaline Peroxide ◽  
Hydrolysis Of ◽  
Peroxide Delignification
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