Enhanced the enzymatic hydrolysis efficiency of wheat straw after combined steam explosion and laccase pretreatment

2012 ◽  
Vol 118 ◽  
pp. 8-12 ◽  
Author(s):  
Weihua Qiu ◽  
Hongzhang Chen
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Steam Explosion

Combined steam-explosion toward vacuum and dilute-acid spraying of wheat straw. Impact of severity factor on enzymatic hydrolysis

2015 ◽  
Vol 78 ◽  
pp. 516-526 ◽  
Author(s):  
Maache-Rezzoug Zoulikha ◽  
Maugard Thierry ◽  
Zhao Jean-Michel Qiuyu ◽  
Armelle Nouviaire ◽  
Rezzoug Sid-Ahmed
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Steam Explosion ◽  
Dilute Acid ◽  
Severity Factor

scholarly journals Optimization of steam explosion parameters for improved biotechnological use of wheat straw

2021 ◽  
Author(s):  
Daniel Sulzenbacher ◽  
Denise Atzmüller ◽  
Felix Hawe ◽  
Manuela Richter ◽  
Alvaro Cristobal-Sarramian ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Steam Explosion ◽  
Raw Materials ◽  
Lignocellulosic Material ◽  
Biotechnological Production ◽  
Industrial Fermentation ◽  
Wheat Straw Hydrolysate ◽  
Biotechnological Processes ◽  
Production Host

AbstractUsing lignocellulosic raw materials as substrate for biotechnological applications has been a focus of research during the last two decades. They contain sugars, which can be used in industrial fermentation processes, in from of polysaccharides (cellulose, hemicellulose). Wheat straw, one representative of lignocellulosic materials, is sustainably and abundantly available, especially in Europe and North America. However, wheat straw, just like any other lignocellulosic material, needs to be pretreated in one way or the other in order to generate sufficient quantities of monosaccharides. One widely used pretreatment for lignocellulosic material is steam explosion combined with enzymatic hydrolysis. In this study, the effects of steam exploding wheat straw in combination with water are presented. By impregnation with water, saccharide yields from subsequent enzymatic hydrolysis increased from 18.8 to 22.6 g L−1 for glucose and 13.8 to 16.4 g L−1 for xylose, respectively. Moreover, the basic steam explosion parameters residence time and temperature were optimized in ranges from 5 to 20 min and 180–200 °C. This further optimization increased the maximum saccharide yield to 41.2 g L−1 for glucose (200 °C, 15 min) and 18.9 g L−1 for xylose (190 °C, 10 min). Finally, the growth of the intensively investigated biotechnological production host Yarrowia lipolytica on hydrolysates derived from different steam explosion parameters was evaluated. Y. lipolytica grew well in media containing up to 90% wheat straw hydrolysate as sole carbon source, demonstrating the potential as substrate for biotechnological processes.

scholarly journals Steam Explosion for Wheat Straw Pretreatment for Sugars Production

Bioethanol ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Pablo Alvira ◽  
María José Negro ◽  
Ignacio Ballesteros ◽  
Alberto González ◽  
Mercedes Ballesteros
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Steam Explosion ◽  
Large Scale ◽  
Degradation Products ◽  
Transport Sector ◽  
High Solids Loading ◽  
Hydrolysis Process ◽  
Pretreatment Conditions ◽  
Pretreated Wheat Straw

AbstractDevelopment of biofuels such as lignocellulosic ethanol represents a sustainable alternative in the transport sector. Wheat straw is a promising feedstock for bioethanol production in Europe due to its large production and high carbohydrates content. In a process to produce cellulosic ethanol, previous to the enzymatic hydrolysis to obtain fermentable sugars and the subsequent fermentation, a pretreatment step to break down the recalcitrance of lignocellulose fiber is essential. In this work, a range of steam explosion pretreatment conditions were evaluated according to different parameters: sugars recovery, degradation products generation, and enzymatic hydrolysis yields. Moreover, the enzymatic hydrolysis process was also studied at high substrate loadings, since operating at high solids loading is crucial for large scale development of ethanol production. Pretreatment at 200°C - 10 min resulted in higher enzymatic hydrolysis yield (91.7%) and overall glucose yields (35.4 g glucose/100 g wheat straw) but also higher production of toxic compound. In turn, the characteristics of the pretreated wheat straw at lower severity (Log R0=3.65) correspond to 190°C and 10 min, with minimal sugars degradation and toxics formation indicated a great potential for maximizing total sugars production by using optimal enzyme combinations including accessory enzymes in the enzymatic hydrolysis step.

Pretreatment of wheat straw by supercritical CO2 and its enzymatic hydrolysis for sugar production

2010 ◽  
Vol 107 (1) ◽  
pp. 61-66 ◽  
Author(s):  
Roozbeh Alinia ◽  
Samyar Zabihi ◽  
Feridun Esmaeilzadeh ◽  
Jamshid Fathi Kalajahi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Wheat Straw ◽  
Supercritical Co2 ◽  
Sugar Production

scholarly journals Effects on Lignin Redistribution in Eucalyptus globulus Fibres Pre-Treated by Steam Explosion: A Microscale Study to Cellulose Accessibility

Biomolecules ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 507
Author(s):  
Eduardo Troncoso-Ortega ◽  
Rosario del P. Castillo ◽  
Pablo Reyes-Contreras ◽  
Patricia Castaño-Rivera ◽  
Regis Teixeira Mendonça ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Eucalyptus Globulus ◽  
Steam Explosion ◽  
Laser Scanning ◽  
Structural Changes ◽  
Micro Particles ◽  
Cellulose Accessibility ◽  
Ft Ir ◽  
Enzymatic Accessibility ◽  
Physical And Chemical

The objective of this study was to investigate structural changes and lignin redistribution in Eucalyptus globulus pre-treated by steam explosion under different degrees of severity (S0), in order to evaluate their effect on cellulose accessibility by enzymatic hydrolysis. Approximately 87.7% to 98.5% of original glucans were retained in the pre-treated material. Glucose yields after the enzymatic hydrolysis of pre-treated material improved from 19.4% to 85.1% when S0 was increased from 8.53 to 10.42. One of the main reasons for the increase in glucose yield was the redistribution of lignin as micro-particles were deposited on the surface and interior of the fibre cell wall. This information was confirmed by laser scanning confocal fluorescence and FT-IR imaging; these microscopic techniques show changes in the physical and chemical characteristics of pre-treated fibres. In addition, the results allowed the construction of an explanatory model for microscale understanding of the enzymatic accessibility mechanism in the pre-treated lignocellulose.

Kinetic model of enzymatic hydrolysis of steam-exploded wheat straw

2012 ◽  
Vol 87 (2) ◽  
pp. 1280-1285 ◽  
Author(s):  
Greta Radeva ◽  
Ivo Valchev ◽  
Stoiko Petrin ◽  
Eva Valcheva ◽  
Petya Tsekova
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Model ◽  
Wheat Straw ◽  
Hydrolysis Of
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