scholarly journals Hybrid Elastin-like Polypeptide–Polyethylene Glycol (ELP-PEG) Hydrogels with Improved Transparency and Independent Control of Matrix Mechanics and Cell Ligand Density

2014 ◽  
Vol 15 (9) ◽  
pp. 3421-3428 ◽  
Author(s):  
Huiyuan Wang ◽  
Lei Cai ◽  
Alexandra Paul ◽  
Annika Enejder ◽  
Sarah C. Heilshorn
Keyword(s):  
Polyethylene Glycol ◽  
Independent Control ◽  
Ligand Density ◽  
Peg Hydrogels ◽  
Matrix Mechanics

scholarly journals Effects of Polyethylene Glycol Spacer Length and Ligand Density on Folate Receptor Targeting of Liposomal Doxorubicin In Vitro

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Kumi Kawano ◽  
Yoshie Maitani
Keyword(s):  
Polyethylene Glycol ◽  
Liposomal Doxorubicin ◽  
Folate Receptor ◽  
In Vitro Cytotoxicity ◽  
Receptor Targeting ◽  
Spacer Length ◽  
Ligand Density ◽  
Molecular Weights ◽  
Folate Receptor Targeting

The folate receptor is an attractive target for selective tumor delivery of liposomal doxorubicin (DXR) because it is abundantly expressed in a large percentage of tumors. This study examined the effect of polyethylene glycol (PEG) spacer length and folate ligand density on the targeting ability of folate-modified liposomes. Liposomes were modified with folate-derivatized PEG-distearoylphosphatidylethanolamine with PEG molecular weights of 2000, 3400, or 5000. The association of DXR-loaded liposomes with KB cells, which overexpress the folate receptor, was evaluated by flow cytometry at various ratios of folate modification. A low ratio of folate modification with a sufficiently long PEG chain showed the highest folate receptor-mediated association with the cells, but did not show the highest in vitro cytotoxicity. DXR release from folate-modified liposomes in endosomes might be different. These findings will be useful for designing folate receptor-targeting carriers.

scholarly journals Guest-host interlinked PEG-MAL granular hydrogels as an engineered cellular microenvironment

2021 ◽  
Author(s):  
Adrienne E Widener ◽  
Mallika S Bhatta ◽  
Thomas E Angelini ◽  
Edward A Phelps
Keyword(s):  
Cell Migration ◽  
Polyethylene Glycol ◽  
Cellular Microenvironment ◽  
Hydrogel Scaffold ◽  
Peg Hydrogels ◽  
Cellular Microenvironments

We report the development of a polyethylene glycol (PEG) hydrogel scaffold that provides the advantages of conventional bulk PEG hydrogels for engineering cellular microenvironments and allows for rapid cell migration....

scholarly journals Influence of Supraphysiologic Biomaterial Stiffness on Ventricular Mechanics and Myocardial Infarct Reinforcement

2020 ◽  
Author(s):  
Ravi K. Ghanta ◽  
Yunge Zhao ◽  
Aarthi Pugazenthi ◽  
Mathew J. Wall ◽  
Lauren N. Russell ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Ventricular Remodeling ◽  
Myocardial Infarct ◽  
Design Criteria ◽  
Peg Hydrogels ◽  
Ventricular Mechanics ◽  
Wide Range ◽  
Adverse Remodeling ◽  
The Impact

ABSTRACTInjectable intramyocardial biomaterials have promise to limit adverse ventricular remodeling through mechanical and biologic mechanisms. While some success has been observed by injecting materials to regenerate new tissue, optimal biomaterial stiffness to thicken and stiffen infarcted myocardium to limit adverse remodeling has not been determined. In this work, we present an in-vivo study of the impact of biomaterial stiffness over a wide range of stiffness moduli on ventricular mechanics. We utilized injectable methacrylated polyethylene glycol (PEG) hydrogels fabricated at 3 different mechanical moduli: 5 kPa (low), 25 kPa (medium/myocardium), and 250 kPa (high/supraphysiologic). We demonstrate that the supraphysiological high stiffness favorably alters post-infarct ventricular mechanics and prevents negative tissue remodeling. Lower stiffness materials do not alter mechanics and thus to be effective, must instead target biological reparative mechanisms. These results may influence rationale design criteria for biomaterials developed for infarct reinforcement therapy.

Multiscale Characterization of the Mechanical Properties of Fibrin and Polyethylene Glycol (PEG) Hydrogels for Tissue Engineering Applications

2021 ◽  
pp. 2100366
Author(s):  
Christian Jose Garcia Abrego ◽  
Lens Dedroog ◽  
Olivier Deschaume ◽  
Jolan Wellens ◽  
Anja Vananroye ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Engineering Applications ◽  
Peg Hydrogels

Optical Features of Lanthanide Luminescent Hydrogel Based on Polyethylene Glycol with Silica Nanoparticles

2021 ◽  
Vol 13 (1) ◽  
pp. 123-130
Author(s):  
Zhihuan Zhao ◽  
Yao Wang ◽  
Haoyang Jiang ◽  
Jixian Liu ◽  
Linjun Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Glycol ◽  
Silica Nanoparticles ◽  
Optoelectronic Devices ◽  
Hybrid Hydrogel ◽  
Peg Hydrogels ◽  
Polymer Hybrid ◽  
Hybrid Hydrogels ◽  
Potential Applications

Inorganic/polymer hybrid hydrogels generally exhibit excellent mechanical properties, while extra function include luminescence are extremely explored due to the further application in sensors and optoelectronic devices. Herein, we report the rare-earth (RE) complex/silica nanoparticles (SNs) luminescent hybrid polyethylene glycol (PEG) hydrogels showing stable luminescence. The hybrid hydrogels coordinated with RE (Eu3+) complex are fabricated via a convenient in situ photocrosslinked procedure. The coordination polymers showed red luminescent color under the 365 nm UV irradiation. Furthermore, the hybrid hydrogel exhibited long luminescence lifetime. These properties of hybrid luminescent hydrogel gave rise to a great improvement in the potential applications as a soft material.

scholarly journals Encapsulation of BSA in hybrid PEG hydrogels: stability and controlled release

RSC Advances ◽  
2021 ◽  
Vol 11 (49) ◽  
pp. 30887-30897
Author(s):  
Corine Tourné-Péteilh ◽  
Maeva Barège ◽  
Mathieu Lions ◽  
Jean Martinez ◽  
Jean-Marie Devoisselle ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Controlled Release ◽  
Bovine Serum Albumin ◽  
Sol Gel ◽  
Fine Tuning ◽  
Peg Hydrogels ◽  
Good Insight ◽  
Hybrid Hydrogels ◽  
Model Protein ◽  
Protein Bovine Serum Albumin

Silylated hybrid hydrogels of polyethylene glycol were designed to trap, stabilize and release a model protein (bovine serum albumin). Fine-tuning sol–gel reactions lead to sustained release of BSA over weeks, with good insight of protein stability.

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

Directional observation on lipid of male nectary of Lindera megaphylla hemsl. by the rapid embedding method with polyethylene glycol (PEG)

1990 ◽  
Vol 48 (3) ◽  
pp. 718-719
Author(s):  
Dai Dalin ◽  
Guo Jianmin
Keyword(s):  
Electron Microscope ◽  
Polyethylene Glycol ◽  
Chemical Reaction ◽  
Traditional Method ◽  
Osmium Tetroxide ◽  
Unsaturated Lipid ◽  
Embedding Method ◽  
Long Period ◽  
New Methods

Lipid cytochemistry has not yet advanced far at the EM level. A major problem has been the loss of lipid during dehydration and embedding. Although the adoption of glutaraldehyde and osmium tetroxide accelerate the chemical reaction of lipid and osmium tetroxide can react on the double bouds of unsaturated lipid to from the osmium black, osmium tetroxide can be reduced in saturated lipid and subsequently some of unsaturated lipid are lost during dehydration. In order to reduce the loss of lipid by traditional method, some researchers adopted a few new methods, such as the change of embedding procedure and the adoption of new embedding media, to solve the problem. In a sense, these new methods are effective. They, however, usually require a long period of preparation. In this paper, we do research on the fiora nectary strucure of lauraceae by the rapid-embedding method wwith PEG under electron microscope and attempt to find a better method to solve the problem mentioned above.

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