scholarly journals Encapsulation of BSA in hybrid PEG hydrogels: stability and controlled release

RSC Advances ◽  
2021 ◽  
Vol 11 (49) ◽  
pp. 30887-30897
Author(s):  
Corine Tourné-Péteilh ◽  
Maeva Barège ◽  
Mathieu Lions ◽  
Jean Martinez ◽  
Jean-Marie Devoisselle ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Controlled Release ◽  
Bovine Serum Albumin ◽  
Sol Gel ◽  
Fine Tuning ◽  
Peg Hydrogels ◽  
Good Insight ◽  
Hybrid Hydrogels ◽  
Model Protein ◽  
Protein Bovine Serum Albumin

Silylated hybrid hydrogels of polyethylene glycol were designed to trap, stabilize and release a model protein (bovine serum albumin). Fine-tuning sol–gel reactions lead to sustained release of BSA over weeks, with good insight of protein stability.

Alteration of water absorption in the THz region traces the onset of fibrillation in proteins

2021 ◽  
Author(s):  
Partha Pyne ◽  
Nirnay Samanta ◽  
Himanshu Gohil ◽  
S. S. Prabhu ◽  
Rajib Kumar Mitra
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Water Absorption ◽  
Bovine Serum ◽  
Terahertz Spectroscopy ◽  
Model Protein ◽  
Protein Bovine Serum Albumin

Using terahertz spectroscopy, we established the alteration of the collective hydration of water during the fibrillation process (native → intermediate → fibril) of a model protein bovine serum albumin.

scholarly journals Efficacy of alcohol/sugar aqueous biphasic system on partition of bovine serum albumin

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Yin Hui Chow ◽  
Alagan Sahlini ◽  
Hui-Suan Ng ◽  
John Chi-Wei Lan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Protein Concentration ◽  
Protein Extraction ◽  
Ftir Analysis ◽  
Model Protein ◽  
Aqueous Biphasic ◽  
The Fourier Transform ◽  
Protein Bovine Serum Albumin

AbstractThe efficacy of alcohol/sugar aqueous biphasic (ABS) system on protein extraction was investigated. A model protein, bovine serum albumin (BSA), was adopted to evaluate the effects of types and concentration of phase-forming components, protein concentration, and system pH on the protein partition efficiency. The 1-propanol/maltose ABS exhibited an overall better partition efficiency of BSA to the alcohol-rich top phase. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01% of BSA were achieved with 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 for 10% (w/w) BSA load. The K and Y of BSA in 1-propanol/maltose ABS was slightly improved with the addition of 3% (w/w) of ionic liquid, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvant that could provide protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.

scholarly journals Honey-Induced Protein Stabilization as Studied by Fluorescein Isothiocyanate Fluorescence

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Yin How Wong ◽  
Habsah Abdul Kadir ◽  
Saad Tayyab
Keyword(s):  
Bovine Serum Albumin ◽  
Thermal Denaturation ◽  
Protein Denaturation ◽  
Fluorescein Isothiocyanate ◽  
Protein Stabilization ◽  
Urea Concentration ◽  
Transition Curve ◽  
Model Protein ◽  
Chemical Coupling ◽  
Protein Bovine Serum Albumin

Protein stabilizing potential of honey was studied on a model protein, bovine serum albumin (BSA), using extrinsic fluorescence of fluorescein isothiocyanate (FITC) as the probe. BSA was labelled with FITC using chemical coupling, and urea and thermal denaturation studies were performed on FITC-labelled BSA (FITC-BSA) both in the absence and presence of 10% and 20% (w/v) honey using FITC fluorescence at 522 nm upon excitation at 495 nm. There was an increase in the FITC fluorescence intensity upon increasing urea concentration or temperature, suggesting protein denaturation. The results from urea and thermal denaturation studies showed increased stability of protein in the presence of honey as reflected from the shift in the transition curve along with the start point and the midpoint of the transition towards higher urea concentration/temperature. Furthermore, the increase inΔGDH2OandΔGD25∘Cin presence of honey also suggested protein stabilization.

Optical Features of Lanthanide Luminescent Hydrogel Based on Polyethylene Glycol with Silica Nanoparticles

2021 ◽  
Vol 13 (1) ◽  
pp. 123-130
Author(s):  
Zhihuan Zhao ◽  
Yao Wang ◽  
Haoyang Jiang ◽  
Jixian Liu ◽  
Linjun Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Glycol ◽  
Silica Nanoparticles ◽  
Optoelectronic Devices ◽  
Hybrid Hydrogel ◽  
Peg Hydrogels ◽  
Polymer Hybrid ◽  
Hybrid Hydrogels ◽  
Potential Applications

Inorganic/polymer hybrid hydrogels generally exhibit excellent mechanical properties, while extra function include luminescence are extremely explored due to the further application in sensors and optoelectronic devices. Herein, we report the rare-earth (RE) complex/silica nanoparticles (SNs) luminescent hybrid polyethylene glycol (PEG) hydrogels showing stable luminescence. The hybrid hydrogels coordinated with RE (Eu3+) complex are fabricated via a convenient in situ photocrosslinked procedure. The coordination polymers showed red luminescent color under the 365 nm UV irradiation. Furthermore, the hybrid hydrogel exhibited long luminescence lifetime. These properties of hybrid luminescent hydrogel gave rise to a great improvement in the potential applications as a soft material.

Controlled Release of Nafcillin Using Biocompatible “Dummy” Molecularly Imprinted Sol-Gel Nanospheres

2015 ◽  
Vol 15 (3) ◽  
pp. 262-270 ◽  
Author(s):  
T. Lopez ◽  
M.A. Francos ◽  
A. Gonzalez ◽  
M.E. Diaz-Garcia ◽  
R. Badia-Laino
Keyword(s):  
Controlled Release ◽  
Sol Gel ◽  
Molecularly Imprinted

scholarly journals Preparation of covalently bonded silica-alginate hybrid hydrogels by SCHIFF base and sol-gel reactions

2021 ◽  
pp. 118186
Author(s):  
Aurora C. Hernández-González ◽  
Lucía Téllez-Jurado ◽  
Luis M. Rodríguez-Lorenzo
Keyword(s):  
Schiff Base ◽  
Sol Gel ◽  
Hybrid Hydrogels ◽  
Covalently Bonded

A novel anhydrous preparation of PEG hydrogels enables high drug loading with biologics for controlled release applications

2021 ◽  
Vol 147 ◽  
pp. 110286
Author(s):  
Christian E. Ziegler ◽  
Moritz Graf ◽  
Sebastian Beck ◽  
Achim M. Goepferich
Keyword(s):  
Controlled Release ◽  
Drug Loading ◽  
Peg Hydrogels ◽  
High Drug ◽  
High Drug Loading

scholarly journals Synthesis, Structural, Morphological and Thermal Characterization of Five Different Silica-Polyethylene Glycol-Chlorogenic Acid Hybrid Materials

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1586
Author(s):  
Michelina Catauro ◽  
Pavel Šiler ◽  
Jiří Másilko ◽  
Roberta Risoluti ◽  
Stefano Vecchio Ciprioti
Keyword(s):  
Polyethylene Glycol ◽  
Chlorogenic Acid ◽  
Hybrid Materials ◽  
Sol Gel ◽  
Antibacterial Properties ◽  
Silica Matrix ◽  
Sem Images ◽  
Fixed Amount ◽  
Organic Components ◽  
Free Material

The present study investigated the structure, morphology, thermal behavior, and bacterial growth analysis of novel three-component hybrid materials synthesized by the sol-gel method. The inorganic silica matrix was weakly bonded to the network of two organic components: a well-known polymer such as polyethylene glycol (PEG, average molar mass of about 4000 g/mol), and an antioxidant constituted by chlorogenic acid (CGA). In particular, a first series was made by a 50 wt% PEG-based (CGA-free) silica hybrid along with two 50 wt% PEG-based hybrids containing 10 and 20 wt% of CGA (denoted as SP50, SP50C10 and SP50C20, respectively). A second series contained a fixed amount of CGA (20 wt%) in silica-based hybrids: one was the PEG-free material (SC20) and the other two contained 12 and 50 wt% of PEG, respectively (SP12C20 and SP50C20, respectively), being the latter already included in the first series. The X-ray diffraction (XRD) patterns and scanning electron microscope (SEM) images of freshly prepared materials confirmed that all the materials were amorphous and homogeneous regardless of the content of PEG or CGA. The thermogravimetric (TG) analysis revealed a higher water content was adsorbed into the two component hybrids (SP50 and SC20) because of the availability of a larger number of H-bonds to be formed with water with respect to those of silica/PEG/CGA (SPC), where silica matrix was involved in these bonds with both organic components. Conversely, the PEG-rich materials (SP50C10 and SP50C20, both with 50 wt% of the polymer) retained a lower content of water. Decomposition of PEG and CGA occurred in almost the same temperature interval regardless of the content of each organic component. The antibacterial properties of the SiO2/PEG/CGA hybrid materials were studied in pellets using either Escherichia coli and Enterococcus faecalis, respectively. Excellent antibacterial activity was found against both bacteria regardless of the amount of polymer in the hybrids.

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