scholarly journals Guest-host interlinked PEG-MAL granular hydrogels as an engineered cellular microenvironment

2021 ◽  
Author(s):  
Adrienne E Widener ◽  
Mallika S Bhatta ◽  
Thomas E Angelini ◽  
Edward A Phelps
Keyword(s):  
Cell Migration ◽  
Polyethylene Glycol ◽  
Cellular Microenvironment ◽  
Hydrogel Scaffold ◽  
Peg Hydrogels ◽  
Cellular Microenvironments

We report the development of a polyethylene glycol (PEG) hydrogel scaffold that provides the advantages of conventional bulk PEG hydrogels for engineering cellular microenvironments and allows for rapid cell migration....

scholarly journals Endothelial Cell Migration on RGD-Peptide-Containing PEG Hydrogels in the Presence of Sphingosine 1-Phosphate

2008 ◽  
Vol 94 (1) ◽  
pp. 273-285 ◽  
Author(s):  
Bradley K. Wacker ◽  
Shannon K. Alford ◽  
Evan A. Scott ◽  
Meghna Das Thakur ◽  
Gregory D. Longmore ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Rgd Peptide ◽  
Endothelial Cell Migration ◽  
Peg Hydrogels ◽  
Sphingosine 1 Phosphate

Covalently grafted VEGF165 in hydrogel models upregulates the cellular pathways associated with angiogenesis

2011 ◽  
Vol 301 (5) ◽  
pp. C1086-C1092 ◽  
Author(s):  
A. M. Porter ◽  
C. M. Klinge ◽  
A. S. Gobin
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Cell Migration ◽  
Endothelial Cell ◽  
Umbilical Vein ◽  
Biological Response ◽  
Biomedical Science ◽  
Endothelial Cell Proliferation ◽  
Peg Hydrogels

Angiogenesis is an important biological response known to be involved in many physiological and pathophysiological situations. Cellular responses involved in the formation of new blood vessels, such as increases in endothelial cell proliferation, cell migration, and the survival of apoptosis-inducing events, have been associated with vascular endothelial growth factor isoform 165 (VEGF165). Current research in the areas of bioengineering and biomedical science has focused on developing polyethylene glycol (PEG)-based systems capable of initiating and sustaining angiogenesis in vitro. However, a thorough understanding of how endothelial cells respond at the molecular level to VEGF165 incorporated into these systems has not yet been established in the literature. The goal of the current study was to compare the upregulation of key intracellular proteins involved in angiogenesis in human umbilical vein endothelial cells (HUVEC) and human microvascular endothelial cells (HMEC) seeded on PEG hydrogels containing grafted VEGF165 and adhesion peptides Arg-Gly-Asp-Ser (RGDS). Our data suggest that the covalent incorporation of VEGF165 into PEG hydrogels encourages the upregulation of signaling proteins responsible for increases in endothelial cell proliferation, cell migration, and the survival after apoptosis-inducing events.

scholarly journals Influence of Supraphysiologic Biomaterial Stiffness on Ventricular Mechanics and Myocardial Infarct Reinforcement

2020 ◽  
Author(s):  
Ravi K. Ghanta ◽  
Yunge Zhao ◽  
Aarthi Pugazenthi ◽  
Mathew J. Wall ◽  
Lauren N. Russell ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Ventricular Remodeling ◽  
Myocardial Infarct ◽  
Design Criteria ◽  
Peg Hydrogels ◽  
Ventricular Mechanics ◽  
Wide Range ◽  
Adverse Remodeling ◽  
The Impact

ABSTRACTInjectable intramyocardial biomaterials have promise to limit adverse ventricular remodeling through mechanical and biologic mechanisms. While some success has been observed by injecting materials to regenerate new tissue, optimal biomaterial stiffness to thicken and stiffen infarcted myocardium to limit adverse remodeling has not been determined. In this work, we present an in-vivo study of the impact of biomaterial stiffness over a wide range of stiffness moduli on ventricular mechanics. We utilized injectable methacrylated polyethylene glycol (PEG) hydrogels fabricated at 3 different mechanical moduli: 5 kPa (low), 25 kPa (medium/myocardium), and 250 kPa (high/supraphysiologic). We demonstrate that the supraphysiological high stiffness favorably alters post-infarct ventricular mechanics and prevents negative tissue remodeling. Lower stiffness materials do not alter mechanics and thus to be effective, must instead target biological reparative mechanisms. These results may influence rationale design criteria for biomaterials developed for infarct reinforcement therapy.

Multiscale Characterization of the Mechanical Properties of Fibrin and Polyethylene Glycol (PEG) Hydrogels for Tissue Engineering Applications

2021 ◽  
pp. 2100366
Author(s):  
Christian Jose Garcia Abrego ◽  
Lens Dedroog ◽  
Olivier Deschaume ◽  
Jolan Wellens ◽  
Anja Vananroye ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Polyethylene Glycol ◽  
Engineering Applications ◽  
Peg Hydrogels

Optical Features of Lanthanide Luminescent Hydrogel Based on Polyethylene Glycol with Silica Nanoparticles

2021 ◽  
Vol 13 (1) ◽  
pp. 123-130
Author(s):  
Zhihuan Zhao ◽  
Yao Wang ◽  
Haoyang Jiang ◽  
Jixian Liu ◽  
Linjun Huang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Glycol ◽  
Silica Nanoparticles ◽  
Optoelectronic Devices ◽  
Hybrid Hydrogel ◽  
Peg Hydrogels ◽  
Polymer Hybrid ◽  
Hybrid Hydrogels ◽  
Potential Applications

Inorganic/polymer hybrid hydrogels generally exhibit excellent mechanical properties, while extra function include luminescence are extremely explored due to the further application in sensors and optoelectronic devices. Herein, we report the rare-earth (RE) complex/silica nanoparticles (SNs) luminescent hybrid polyethylene glycol (PEG) hydrogels showing stable luminescence. The hybrid hydrogels coordinated with RE (Eu3+) complex are fabricated via a convenient in situ photocrosslinked procedure. The coordination polymers showed red luminescent color under the 365 nm UV irradiation. Furthermore, the hybrid hydrogel exhibited long luminescence lifetime. These properties of hybrid luminescent hydrogel gave rise to a great improvement in the potential applications as a soft material.

Covalently immobilized RGD gradient on PEG hydrogel scaffold influences cell migration parameters

2010 ◽  
Vol 6 (7) ◽  
pp. 2532-2539 ◽  
Author(s):  
D. Guarnieri ◽  
A. De Capua ◽  
M. Ventre ◽  
A. Borzacchiello ◽  
C. Pedone ◽  
...  
Keyword(s):  
Cell Migration ◽  
Hydrogel Scaffold

Dot-like focal contacts in adherent eosinophils, their redistribution into peripheral belts, and correlated effects on cell migration and protected zone formation

1996 ◽  
Vol 109 (8) ◽  
pp. 2169-2177
Author(s):  
A. Tourkin ◽  
M. Bonner ◽  
E. Mantrova ◽  
E.C. LeRoy ◽  
S. Hoffman
Keyword(s):  
Cell Migration ◽  
Polyethylene Glycol ◽  
Control Cell ◽  
Permeability Barrier ◽  
Integrin Subunit ◽  
Zone Formation ◽  
Focal Contacts ◽  
Low Concentrations ◽  
Functional Consequences ◽  
And Behavior

We have examined the organization of F-actin and focal contacts in eosinophils and the functional consequences correlated with their predistribution. In activated eosinophils adherent to laminin, F-actin is localized in large, uniformly distributed, dot-like structures. Co-localized with the F-actin are a variety of typical components of focal contacts including: the alpha 6 and beta 1 integrins subunits (which mediate eosinophil adhesion to laminin), vinculin, talin, pp125FAK, paxillin, and tyrosine phosphorylated proteins, but not the beta 2 integrin subunit which is not involved in eosinophil adhesion to laminin. In unactivated eosinophils on laminin (which do not adhere well) and in activated eosinophils on non-adhesive surfaces (human serum albumin, tenascin, or a mixture of tenascin and laminin), dot-like accumulations of F-actin do not form. When activated eosinophils are incubated on laminin in the presence of low concentrations of 1,1′-ethylidenebis(L-tryptophan), a remarkable rearrangement occurs. Both conventional and confocal microscopy suggest that the dot-like structures rearrange from a uniform distribution into thick, peripheral belts surrounding each cell with few dot-like structures internal to the belt. Functional consequences occur as a result of this rearrangement of focal contacts and the cytoskeleton. The belt of focal contacts acts as a size-dependent permeability barrier; fluorescent 8 kDa polyethylene glycol does not label the substrate underneath the cells whereas 3.5 kDa polyethylene glycol labels the substrate uniformly. The formation of belts of focal contacts also blocks the ability of eosinophils to migrate on laminin in response to a chemotactic gradient. While the mechanism of 1,1′-ethylidenebis(L-tryptophan) action remains to be determined, experiments indicate that the function of 1,1′-ethylidenebis(L-tryptophan) is distinct from the function of drugs that alter tyrosine kinase and serine/threonine kinase activities. Our observations reveal a novel mechanism for regulating cell behavior in invasive cells in which the rearrangement of focal contacts and the associated cytoskeleton may control cell migration and the sequestration of secreted cytotoxic and degradatory molecules. We propose that 1,1′-ethylidenebis(L-tryptophan) may mimic the function of an endogenous regulator of eosinophil morphology and behavior.

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