Dependence of the Activity of Phospholipase Cβ on Surface Pressure and Surface Composition in Phospholipid Monolayers and Its Implications for Their Regulation†

Biochemistry ◽  
1997 ◽  
Vol 36 (4) ◽  
pp. 848-855 ◽  
Author(s):  
Stephen R. James ◽  
Andrew Paterson ◽  
T. Kendall Harden ◽  
Rudy A. Demel ◽  
C. Peter Downes
Keyword(s):  
Surface Pressure ◽  
Surface Composition ◽  
Phospholipid Monolayers

Imaging Nanometer Scale Optical Heterogeneities in Phospholipid Monolayers Deposited on Metal Island Films

2006 ◽  
Vol 6 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Anatoli Ianoul ◽  
Natalia Strekal ◽  
Sergei Maskevich
Keyword(s):  
Surface Pressure ◽  
Near Field ◽  
Lipid Monolayer ◽  
Nanometer Scale ◽  
Island Film ◽  
Langmuir Blodgett ◽  
Phospholipid Monolayers ◽  
Metal Island ◽  
Fluorescence Images ◽  
Island Films

Near-field scanning optical microscopy was applied to study the distribution of fluorescently labeled phospholipid monolayers deposited on the surface of gold island films by the Langmuir-Blodgett technique. Nanometer scale (∼50 nm)optical heterogeneities were observed in near-field fluorescence images of the monolayer deposited at 10 mN/m surface pressure. At higher surface pressure (30 mN/m)the heterogeneities became less pronounced. Overlaying of the near-field transmission and fluorescence images from the same area of the sample shows local transmission of gold island film is at a minimum where the fluorescence of the lipid monolayer is at a maximum. It was concluded that coverage of the metal island film by the Langmuir-Blodgett phospholipid monolayer is incomplete, and lipid molecules are preferentially localized in crevices of the film.

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