Steady-State Kinetics and Spectroscopic Characterization of Enzyme–tRNA Interactions for the Non-Heme Diiron tRNA-Monooxygenase, MiaE

Biochemistry ◽  
2014 ◽  
Vol 54 (2) ◽  
pp. 363-376 ◽  
Author(s):  
Bishnu P. Subedi ◽  
Andra L. Corder ◽  
Siai Zhang ◽  
Frank W. Foss ◽  
Brad S. Pierce
Keyword(s):  
Steady State ◽  
Spectroscopic Characterization ◽  
Steady State Kinetics

scholarly journals Spectroscopic characterization of the warfarin drug-binding site of folded and unfolded human serum albumin anchored on gold nanoparticles: effect of bioconjugation on the loading capacity

RSC Advances ◽  
2018 ◽  
Vol 8 (14) ◽  
pp. 7523-7532 ◽  
Author(s):  
Saba A. J. Sulaiman ◽  
Tanujjal Bora ◽  
Osama K. Abou-Zied
Keyword(s):  
Gold Nanoparticles ◽  
Steady State ◽  
Ultrafast Spectroscopy ◽  
Drug Loading ◽  
Spectroscopic Characterization ◽  
Binding Activity ◽  
Drug Binding ◽  
Loading Capacity ◽  
Drug Binding Site

This work investigates the steady-state and ultrafast spectroscopy of bioconjugated gold nanoparticles and the implications on the protein binding activity and drug-loading capacity.

Characterization of recombinant human acetyl-CoA carboxylase-2 steady-state kinetics

2009 ◽  
Vol 1794 (6) ◽  
pp. 961-967 ◽  
Author(s):  
Virendar K. Kaushik ◽  
Michael Kavana ◽  
Jessica M. Volz ◽  
Stephen C. Weldon ◽  
Susan Hanrahan ◽  
...  
Keyword(s):  
Steady State ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Steady State Kinetics

Steady-state and time-resolved fluorescence spectroscopic characterization of urine of healthy subjects and cervical cancer patients

2014 ◽  
Vol 19 (3) ◽  
pp. 037003 ◽  
Author(s):  
Ramu Rajasekaran ◽  
Prakasa Rao Aruna ◽  
Dornadula Koteeswaran ◽  
Ganesan Bharanidharan ◽  
Munusamy Baludavid ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Steady State ◽  
Cancer Patients ◽  
Healthy Subjects ◽  
Spectroscopic Characterization ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Steady state and time-resolved fluorescence spectroscopic characterization of normal and cancerous urine

2013 ◽  
Author(s):  
Ramu Rajasekaran ◽  
Prakasa Rao Aruna ◽  
Munusamy Balu David ◽  
Dornadula Koteeswaran ◽  
Kulandaivel Muthuvelu ◽  
...  
Keyword(s):  
Steady State ◽  
Spectroscopic Characterization ◽  
Time Resolved Fluorescence ◽  
Time Resolved

scholarly journals Pseudomonas aeruginosaC5-Mannuronan Epimerase:  Steady-State Kinetics and Characterization of the Product†

Biochemistry ◽  
2006 ◽  
Vol 45 (2) ◽  
pp. 552-560 ◽  
Author(s):  
Agoston Jerga ◽  
Aniruddha Raychaudhuri ◽  
Peter A. Tipton
Keyword(s):  
Steady State ◽  
Steady State Kinetics

scholarly journals Purification and characterization of two glutathione S-aryltransferase activities from rat liver.

1975 ◽  
Vol 147 (3) ◽  
pp. 513-522 ◽  
Author(s):  
P Askelöf ◽  
C Guthenberg ◽  
I Jakobson ◽  
B Mannervik
Keyword(s):  
Steady State ◽  
Rat Liver ◽  
Substrate Concentration ◽  
Thiol Group ◽  
Purification And Characterization ◽  
Molecular Weights ◽  
Enzymic Reaction ◽  
Steady State Kinetics

Two forms of glutathione S-aryltransferase were purified from rat liver. The only differences noted between the two forms were in the chromatographic and electrophoretic properties, which permitted the separation of the two species. The molecular weights of the enzyme and its subunits were estimated as about 50000 and 23000 respectively. The steady-state kinetics did no follow Michaelis-Menten kinetics when one substrate concentration was kept constant while the second substrate concentration was varied. Several S-substituted GSH derivatives were tested as inhibitors of the enzymic reaction. The enzyme was inactivated by thiol-group reagents.

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