Steady state fluorescence spectroscopic characterization of normal and diabetic urine at selective excitation wavelength 280 nm

2016 ◽  
Author(s):  
Anjana Kesavan ◽  
Rekha Pachaiappan ◽  
Prakasa Rao Aruna ◽  
Singaravelu Ganesan
Keyword(s):  
Steady State ◽  
Spectroscopic Characterization ◽  
Selective Excitation ◽  
Excitation Wavelength ◽  
Steady State Fluorescence

scholarly journals Spectroscopic characterization of the warfarin drug-binding site of folded and unfolded human serum albumin anchored on gold nanoparticles: effect of bioconjugation on the loading capacity

RSC Advances ◽  
2018 ◽  
Vol 8 (14) ◽  
pp. 7523-7532 ◽  
Author(s):  
Saba A. J. Sulaiman ◽  
Tanujjal Bora ◽  
Osama K. Abou-Zied
Keyword(s):  
Gold Nanoparticles ◽  
Steady State ◽  
Ultrafast Spectroscopy ◽  
Drug Loading ◽  
Spectroscopic Characterization ◽  
Binding Activity ◽  
Drug Binding ◽  
Loading Capacity ◽  
Drug Binding Site

This work investigates the steady-state and ultrafast spectroscopy of bioconjugated gold nanoparticles and the implications on the protein binding activity and drug-loading capacity.

Steady-State Kinetics and Spectroscopic Characterization of Enzyme–tRNA Interactions for the Non-Heme Diiron tRNA-Monooxygenase, MiaE

Biochemistry ◽  
2014 ◽  
Vol 54 (2) ◽  
pp. 363-376 ◽  
Author(s):  
Bishnu P. Subedi ◽  
Andra L. Corder ◽  
Siai Zhang ◽  
Frank W. Foss ◽  
Brad S. Pierce
Keyword(s):  
Steady State ◽  
Spectroscopic Characterization ◽  
Steady State Kinetics

The Steady-State and Decay Characteristics of Protein Tryptophan Fluorescence from Algae

1988 ◽  
Vol 42 (8) ◽  
pp. 1405-1412 ◽  
Author(s):  
M. Baek ◽  
W. H. Nelson ◽  
P. E. Hargraves ◽  
J. F. Tanguay ◽  
S. L. Suib
Keyword(s):  
Steady State ◽  
Tryptophan Fluorescence ◽  
Fluorescence Decay ◽  
Direct Application ◽  
Fluorescence Lifetimes ◽  
Bacterial Protein ◽  
Steady State Fluorescence ◽  
Tryptophan Residues ◽  
Rapid Characterization

The intrinsic steady-state fluorescence due to tryptophan has been obtained from monospecific cultures of fourteen plankton algae of various genera. Fluorescence decay profiles of protein tryptophan residues were obtained for eight marine plankton algae. Each organism exhibits a strong maximum in its emission spectrum at 320–340 nm when excited at 290 nm. Iodide quenching and denaturization experiments with 8 M urea provide strong evidence for the assignment of the 320–340 nm fluorescence to protein tryptophan. Most importantly, the decay of this bacterial protein tryptophan fluorescence has been described. The observation that characteristic protein-tryptophan fluorescence lifetimes have been obtained for each organism suggests that measurements of fluorescence lifetimes may be helpful in the rapid characterization of algae. Direct application will likely be found in combination with the measurement of other luminescence parameters.

Steady-state and time-resolved fluorescence spectroscopic characterization of urine of healthy subjects and cervical cancer patients

2014 ◽  
Vol 19 (3) ◽  
pp. 037003 ◽  
Author(s):  
Ramu Rajasekaran ◽  
Prakasa Rao Aruna ◽  
Dornadula Koteeswaran ◽  
Ganesan Bharanidharan ◽  
Munusamy Baludavid ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Steady State ◽  
Cancer Patients ◽  
Healthy Subjects ◽  
Spectroscopic Characterization ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Steady-State Fluorescence of Polystyrene Plasticized by Supercritical Carbon Dioxide

1996 ◽  
Vol 50 (6) ◽  
pp. 740-746 ◽  
Author(s):  
Ming Li ◽  
Frank V. Bright
Keyword(s):  
Molecular Weight ◽  
Steady State ◽  
Conformational Changes ◽  
Fluorescence Intensity ◽  
Excitation Wavelength ◽  
Fluorescence Intensity Ratio ◽  
Steady State Fluorescence ◽  
Proposed Model ◽  
Major Increase ◽  
Reduced Density

A steady-state fluorescence study of low-molecular-weight polystyrene (MW = 1060 g/mol and 13,000 g/mol) plasticized in supercritical CO2 is reported. In addition to excitation wavelength, molecular weight, and polystyrene concentration dependencies, CO2 density also strongly affects the emission spectral contours. A major increase in the steady-state fluorescence intensity and a significant decrease in the polystyrene 320- to 365-nm fluorescence intensity ratio are observed when CO2 density is increased. Concentration and conformational changes in the polystyrene molecules are used to explain the observations, and these results are proposed to arise from changes in the plasticization power of supercritical CO2 over the density range studied. A theoretical model is proposed that is based on the assumption that, at low CO2 densities and low polymer concentrations, polystyrene intermolecular interactions are negligible. The proposed model is able to fit our observed fluorescence data from a CO2 reduced density of 0.3 to 1.4.

Steady state and time-resolved fluorescence spectroscopic characterization of normal and cancerous urine

2013 ◽  
Author(s):  
Ramu Rajasekaran ◽  
Prakasa Rao Aruna ◽  
Munusamy Balu David ◽  
Dornadula Koteeswaran ◽  
Kulandaivel Muthuvelu ◽  
...  
Keyword(s):  
Steady State ◽  
Spectroscopic Characterization ◽  
Time Resolved Fluorescence ◽  
Time Resolved

Study on Removal Efficiency and Fluorescence Characteristics of Humus in Landfill Leachate Treated by Combined Process

2011 ◽  
Vol 233-235 ◽  
pp. 667-672
Author(s):  
Zhi Ping Liu ◽  
Jin Song Guo ◽  
Fang Fang
Keyword(s):  
Removal Efficiency ◽  
Landfill Leachate ◽  
Three Dimensional ◽  
Spectroscopic Characterization ◽  
Excitation Wavelength ◽  
Combined Process ◽  
Effective Removal ◽  
Fluorescence Characteristics ◽  
Fluorescence Peak

The removal efficiency and three-dimensional excitation emission matrix(3DEEM) fluorescence spectroscopic characterization of landfill leachate humus (HA and FA) in the Fenton-SBR process was studied. It was concluded that the combined Fenton-SBR process was available for effective removal of leachate humus, and the removal rates for HA and FA was 87.3% and 88.2%, respectively. The major fluorescence peaks observed in the leachate combined process was protein-like fluorescence peak a (Ex/Em=280nm/345nm), humic-like fluorescence peak b (Ex/Em=295/400nm), short excitation wavelength tryptophan-like fluorescence peak c (Ex/Em= 230nm/340nm) and ultraviolet band fulvic acid-like fluorescence peak d (Ex/Em=235nm/400nm). Types of fluorescence peak of HA and FA reduced and the intensities decreased after combined process treatment. The capability for removal of different components of humus was determined by the process units adopted.

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