Sodium Dodecyl Sulfate−Polyacrylamide Gel Electrophoresis and Förster Resonance Energy Transfer Suggest Weak Interactions between Fibroblast Growth Factor Receptor 3 (FGFR3) Transmembrane Domains in the Absence of Extracellular Domains and Ligands†

Biochemistry ◽  
2005 ◽  
Vol 44 (1) ◽  
pp. 352-360 ◽  
Author(s):  
Edwin Li ◽  
Min You ◽  
Kalina Hristova
Keyword(s):  
Energy Transfer ◽  
Gel Electrophoresis ◽  
Fibroblast Growth Factor Receptor ◽  
Weak Interactions ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Resonance Energy Transfer ◽  
Growth Factor Receptor ◽  
Resonance Energy ◽  
Fibroblast Growth

Photoinduced energy and electron transfer in micellar solutions of methylene blue

1988 ◽  
Vol 53 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Viktor Řehák ◽  
Yvona Rybová
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Energy Transfer ◽  
Ethylenediaminetetraacetic Acid ◽  
Sodium Dodecyl ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Quenching Rate ◽  
Pyrene Fluorescence ◽  
Energy And Electron Transfer

In the micellar medium of sodium dodecyl sulphate the monomer-dimer equilibrium of methylene blue is shifted markedly in favour of the monomer. Methylene blue quenches the fluorescence of pyrene solvated in micelles, which is explained in terms of resonance energy transfer. Based on the theoretical model, the pyrene fluorescence quenching constant in the micelles was determined (kqm = 1.8 106 s-1) and the average pyrene-methylene blue interaction distance for the singlet-singlet energy transfer estimated. The T1 → Tn absorption of methylene blue is quenched by pyrene and tetrasodium salt of ethylenediaminetetraacetic acid (EDTA); the quenching rate constants corresponding to the electron transfer are 5 106 and 2.05 108 1 mol-1 s-1, respectively.

The Fluorescence Characteristic of AO-RB-SDS-GMFX and the Determination of Gemifloxacin

2022 ◽  
Vol 905 ◽  
pp. 169-173
Author(s):  
Hui Ping Xi ◽  
Xiu Wei Fang ◽  
Bao Hua Wu
Keyword(s):  
Energy Transfer ◽  
High Performance ◽  
Sodium Dodecyl ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Effective Energy ◽  
Buffer Solution ◽  
Indirect Determination ◽  
Effective Energy Transfer

In Britton Robinson (B-R) buffer solution with pH = 7.00 and sodium dodecyl sulfate (SDS) medium, an effective energy transfer between acridine orange (AO) and Rhodamine B (RB) can occur, which can enhance the fluorescence of RB. The addition of Gemifloxacin (GMFX) can quench the fluorescence of RB. So a new method for the indirect determination of Gemifloxacin was established by AO-RB fluorescence resonance energy transfer. This method was applied to the determination of Gemifloxacin tablets. The results were consistent with those of high performance liquid chromatography (HPLC) . Experiments show that this method is simple, rapid, accurate and sensitive.

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