Universal programmable portable measurement device for diagnostics and monitoring of hydraulic and pneumatic equipment

The paper presents a new universal programmable portable measuring device (PMD) intended for the diagnostic and monitoring of hydraulic and pneumatic equipment. PMD offers a simple, complete, and efficient remote monitoring and diagnostic solution for hydraulic and pneumatic equipment. PMD is designed for measurement, recording, and processing of data as well as graphical visualization of registered results on a test stand or in the operating site of hydraulic and pneumatic equipment. PMD is designed to measure parameters used in servicing, repairing, monitoring, and diagnosing hydraulic and pneumatic equipment. Usually, fluidic characteristic quantities (pressure, flow rate, temperature), mechanical quantities (position, linear speed, rotational speed, force, torque, shaft power), and electrical quantities (voltage, current, power) is measured. PMD with WiFi communication is adopted to transfer measurement data via the Industry Internet of Things (IIoT) technology to remote online monitoring and diagnostic hydraulic and pneumatic equipment. Sensors, controllers, and other devices are connected to the computing gateway via their respective protocols.

Experimental investigation of local heat transfer measurement having inclined discrete ribs of solar air heater duct using LCT technique

The steady-state experiment using liquid crystal thermography has been conducted for the analysis of Nusselt number distribution over the absorber surface of solar air heater duct having a gap with the staggered arrangement in inclined rib geometry to recuperate its thermal performance. The heat transfer experiment is performed with uniform heat flux and thermo-chromic liquid crystal is utilized to show the temperature distribution profile over the ribbed surfaces of the rectangular duct of aspect ratio of 5. The colored pattern image of TLCs was acquired using a 3CCD camera and exported to a TIFF file format using frame grabbing SOFTWARE SAPERALT which was further processed to get HSI values. The flow parameters considered in this present investigation are Re, d/W, and g/e varied from 4000-12500,0.15-0.45 and 1-4 respectively. Experiments has been performed with fixed P/e, r/e, p′/P, a and e/Dh of 10, 2, 0.6, 60° and 0.0303 respectively. The influence of relative gap position and relative gap width on flow pattern has been analyzed. The maximum augmentation in Nu and f over the smooth duct was obtained as 4.01 and 4.28 times respectively at the optimum value of d/W = 0.35 and g/e = 2 under similar flow conditions. The maximum value of THPP obtained at d/W & g/e of 0.35 & 2 respectively and Reynolds number of 12445.

Dynamic Shape Transformation of a DNA Scaffold Applied for an Enzyme Nanocarrier

Structural programmability and accurate addressability of DNA nanostructures are ideal characteristics for the platform of arranging enzymes with the nanoscale precision. In this study, a three-dimensional DNA scaffold was designed to enable a dynamic shape transition from an open plate-like structure to its closed state of a hexagonal prism structure. The two domains in the open state were folded together to transform into the closed state by hybridization of complementary short DNA closing keys at both of the facing edges in over 90% yield. The shape transformation of the DNA scaffold was extensively studied by means of the fluorescence energy transfer measurement, atomic force microscope images, and agarose gel electrophoretic analyses. A dimeric enzyme xylitol dehydrogenase was assembled on the DNA scaffold in its open state in a high-loading yield. The enzyme loaded on the scaffold was subsequently transformed to its closed state by the addition of short DNA closing keys. The enzyme encapsulated in the closed state displayed comparable activity to that in the open state, ensuring that the catalytic activity of the enzyme was well maintained in the DNA nanocarrier. The nanocarrier with efficient encapsulation ability is potentially applicable for drug delivery, biosensing, biocatalytic, and diagnostic tools.

Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules.