Isolation and fractionation of yeast nucleic acids. II. Rapid isolation of mitochrondrial deoxyribonucleic acid by poly(L-lysine) kieselguhr chromatography

Biochemistry ◽  
1972 ◽  
Vol 11 (25) ◽  
pp. 4853-4858 ◽  
Author(s):  
David B. Finkelstein ◽  
John Blamire ◽  
Julius Marmur
Keyword(s):  
Nucleic Acids ◽  
Deoxyribonucleic Acid ◽  
Rapid Isolation

III. Functions of short lifetime structures at large 9: case of nucleic acids

2018 ◽  
Vol 18 (3) ◽  
pp. 205-210
Author(s):  
Koichi Nishigaki
Keyword(s):  
Nucleic Acids ◽  
Deoxyribonucleic Acid ◽  
Structure And Function ◽  
The Poor ◽  
Methodological Difficulty ◽  
Short Lifetime ◽  
Transient Structures ◽  
And Function ◽  
Functional Rnas

Abstract The short lifetime structures of nucleic acids are not well studied because of the poor recognition of their importance and the methodological difficulty. In case of proteins, which are a type of single-stranded biopolymers, the essential roles of their transient structures are well established. Therefore, the role of transient structures of nucleic acids is, naturally, of great interest. There have been multiple reports on the function-related unstable (transient) structures of single-stranded nucleotides, though not as many as at present. Recent methodological advances are now enabling us to observe structures with ultra-short lifetime (less than a nanosecond). On the other hand, the biological importance of transient structures of ribonucleicacid (RNA) is increasingly recognized because of the findings of novel functional RNAs such as microRNA. Therefore, the time has come to tackle the structure and function dynamic of RNA/deoxyribonucleic acid in relation to their transient, unstable structures. The specific properties of rapidity and diversity are hypothesized to be involved in unexplored phenomena in neuroscience.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

The reaction of the psoralens with deoxyribonucleic acid

1984 ◽  
Vol 17 (1) ◽  
pp. 1-44 ◽  
Author(s):  
John E. Hearst ◽  
Stephen T. Isaacs ◽  
David Kanne ◽  
Henry Rapoport ◽  
Kenneth Straub
Keyword(s):  
Natural Selection ◽  
Nucleic Acid ◽  
Nucleic Acids ◽  
Chemical Modification ◽  
Deoxyribonucleic Acid ◽  
Molecular Level ◽  
Virus Particles ◽  
Cross Links ◽  
High Concentrations

Psoralen photochemistry is specific for nucleic acids and is better understood at the molecular level than are all other methods of chemical modification of nucleic acids. These compounds are used both for in vivo structure analysis and for photochemotherapy since they easily penetrate both cells and virus particles. Apparently, natural selection has selected for membrane and virus penetrability during the evolution of these natural products. Most cells are unaffected by relatively high concentrations of psoralens in the absence of ultraviolet light, and the metabolites of the psoralens have thus far not created a problem. Finally, psoralens form both monoadduct and cross-links in nucleic acid helices, the yield of each being easily controlled by the conditions used during the photochemistry.

scholarly journals MagaZorb: A Simple Tool for Rapid Isolation of Viral Nucleic Acids

2010 ◽  
Vol 201 (s1) ◽  
pp. S37-S41 ◽  
Author(s):  
Dokhi Nargessi ◽  
Chin‐Yih Ou
Keyword(s):  
Nucleic Acids ◽  
Rapid Isolation ◽  
Simple Tool

scholarly journals Effect of denervation on the synthesis of ribonucleic acid and deoxyribonucleic acid in rat diaphragm muscle

1968 ◽  
Vol 108 (2) ◽  
pp. 177-183 ◽  
Author(s):  
K L Manchester ◽  
E J Harris
Keyword(s):  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Orotic Acid ◽  
Deoxyribonucleic Acid ◽  
Diaphragm Muscle ◽  
Unit Mass ◽  
Rat Diaphragm ◽  
The Third

1. The effect of unilateral denervation of rat diaphragm muscle on its content of nucleic acids and their incorporation of precursors was investigated. 2. After denervation the paralysed hemidiaphragm hypertrophies and within 3 days its content of RNA increases considerably. The concentration of DNA/unit mass remains fairly constant. 3. During this period there is some increase in the rate of incorporation of [14C]adenine into RNA, whereas there is some diminution in the rate of incorporation of [14C]orotic acid. 4. Incorporation of [14C]adenine and [3H]thymidine into DNA is much increased in the paralysed tissue, reaching its maximum by about the third day, but returning to normal by the tenth. 5. The significance of these results in relation to the hypertrophy after denervation is discussed.

Sign in / Sign up

Export Citation Format

Share Document