Developmental changes in microtubule protein of chick brain

Biochemistry ◽  
1973 ◽  
Vol 12 (8) ◽  
pp. 1476-1482 ◽  
Author(s):  
J. R. Bamburg ◽  
E. M. Shooter ◽  
L. Wilson
Keyword(s):  
Developmental Changes ◽  
Chick Brain ◽  
Microtubule Protein

scholarly journals Kinetics of GTP hydrolysis during the assembly of chick brain MAP2-tubulin microtubule protein

1991 ◽  
Vol 277 (1) ◽  
pp. 239-243 ◽  
Author(s):  
R G Burns
Keyword(s):  
Initial Rate ◽  
Molar Concentration ◽  
Microtubule Assembly ◽  
Chick Brain ◽  
Gtp Hydrolysis ◽  
Microtubule Protein ◽  
Rate Of Hydrolysis ◽  
Kinetics Of

The kinetics of GTP hydrolysis during microtubule assembly have been examined using chick brain MAP2-tubulin microtubule protein in a NaCl-supplemented buffer. The elongating microtubules terminate in a ‘GTP cap’, since the kinetics of GTP hydrolysis are slower than those of subunit addition. GTP hydrolysis is (a) stoichiometric, (b) occurs as a vectorial wave as the initial rate of hydrolysis is proportional to the molar concentration of microtubule ends and not to the initial rate of subunit addition, and (c) either does not occur, or occurs only at a much lower rate, in the terminal subunits.

Pre- and post-hatching developmental changes in β-adrenoceptor subtypes in chick brain

1998 ◽  
Vol 111 (2) ◽  
pp. 159-167 ◽  
Author(s):  
Raquel Revilla ◽  
Carlos Fernández-López ◽  
Victoria Revilla ◽  
Arsenio Fernández-López
Keyword(s):  
Developmental Changes ◽  
Chick Brain

scholarly journals Assembly of chick brain MAP2-tubulin microtubule protein. Characterization of the protein and the MAP2-dependent addition of tubulin dimers

1991 ◽  
Vol 277 (1) ◽  
pp. 231-238 ◽  
Author(s):  
R G Burns
Keyword(s):  
Dynamic Instability ◽  
Protein Characterization ◽  
Minor Components ◽  
Chick Brain ◽  
Stoichiometric Mixture ◽  
Pseudo First Order Reaction ◽  
First Order ◽  
Microtubule Protein ◽  
Assembly Kinetics

The principle proteins present in twice-cycled chick brain microtubule protein were characterized. The protein consists of a stoichiometric mixture of MAP2 and tubulin, together with a number of minor components. Its composition remains unaltered after a third cycle of assembly in a buffer supplemented with 67 mM-NaCl, with the exception of the phosphorylation of MAP2 to a low level (congruent to 1 mol.mol-1). The inclusion of 67 mM-NaCl dissociates the MAP2-tubulin oligomers, and restricts the assembly to the MAP2-dependent addition and loss of tubulin dimers, such that the assembly kinetics approximate to a simple pseudo-first-order reaction. The assembled microtubules exhibit dynamic instability, with no evidence for end-to-end annealing.

scholarly journals Phosphorylation of the microtubule-associated protein MAP2 by GTP

1984 ◽  
Vol 224 (2) ◽  
pp. 623-627 ◽  
Author(s):  
R G Burns ◽  
K Islam
Keyword(s):  
Steady State ◽  
Protein Kinase ◽  
Cyclic Amp ◽  
Kinetic Parameters ◽  
Microtubule Assembly ◽  
Chick Brain ◽  
Chemically Modified ◽  
Microtubule Protein ◽  
Independent Protein

The chick brain microtubule-associated protein MAP2 can be phosphorylated in vitro to the extent of 12 mol/mol with GTP at the same sites as can be labelled by the cyclic AMP-independent protein kinase utilizing [gamma-32P]ATP as the phosphoryl donor. Consequently, the microtubule protein is chemically modified by the conditions usually employed for studies of microtubule assembly, so that the derived kinetic parameters may not relate to steady-state conditions.

scholarly journals Assembly of chick brain MAP2-tubulin microtubule protein. Analysis of tubulin subunit flux rates by immunofluorescence microscopy

1991 ◽  
Vol 277 (1) ◽  
pp. 245-253 ◽  
Author(s):  
M F Symmons ◽  
R G Burns
Keyword(s):  
Close Agreement ◽  
Immunofluorescence Microscopy ◽  
Dynamic Instability ◽  
Theoretical Models ◽  
Chick Brain ◽  
Gtp Hydrolysis ◽  
Tubulin Subunit ◽  
Microtubule Protein ◽  
Cap Model ◽  
Microscopy Method

A filter-based immunofluorescence-microscopy method for obtaining microtubule lengths has been developed and evaluated. Kinetic constants and mean lengths obtained show close agreement with those obtained by complementary methods applied to chick brain MAP2-tubulin microtubule protein in NaCl-supplemented buffer. The filter-based method has been used to estimate tubulin subunit flux (Jon) resulting from isothermal dilution of microtubule populations to various free tubulin concentrations, (c). This experimental Jon(c) plot is significantly different from that predicted by a variety of theoretical models, but is consistent with a ‘lateral cap’ model of dynamic instability [Bayley, Schilstra & Martin (1990) J. Cell. Sci. 95, 33-48] adapted to accommodate the observed vectorial GTP hydrolysis.

Spherulitic crystal growth in the prodissoconch larval of Crassostrea virginica

1983 ◽  
Vol 41 ◽  
pp. 518-519
Author(s):  
George G. Cocks ◽  
Louis Leibovitz ◽  
DoSuk D. Lee
Keyword(s):  
Crystal Structure ◽  
Crassostrea Virginica ◽  
Crystal Orientation ◽  
Developmental Changes ◽  
Gastrula Stage ◽  
Orthorhombic Crystal ◽  
Orthorhombic Crystal Structure ◽  
Stages Of Growth ◽  
Early Stages ◽  
Initial Deposition

Our understanding of the structure and the formation of inorganic minerals in the bivalve shells has been considerably advanced by the use of electron microscope. However, very little is known about the ultrastructure of valves in the larval stage of the oysters. The present study examines the developmental changes which occur between the time of conception to the early stages of Dissoconch in the Crassostrea virginica(Gmelin), focusing on the initial deposition of inorganic crystals by the oysters.The spawning was induced by elevating the temperature of the seawater where the adult oysters were conditioned. The eggs and sperm were collected separately, then immediately mixed for the fertilizations to occur. Fertilized animals were kept in the incubator where various stages of development were stopped and observed. The detailed analysis of the early stages of growth showed that CaCO3 crystals(aragonite), with orthorhombic crystal structure, are deposited as early as gastrula stage(Figuresla-b). The next stage in development, the prodissoconch, revealed that the crystal orientation is in the form of spherulites.

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