Rapid labeling of adenosine triphosphate by phosphorus-32-labeled inorganic phosphate and the exchange of phosphate oxygens as related to conformational coupling in oxidative phosphorylation

Biochemistry ◽  
1975 ◽  
Vol 14 (2) ◽  
pp. 392-398 ◽  
Author(s):  
Richard L. Cross ◽  
Paul D. Boyer
Keyword(s):  
Oxidative Phosphorylation ◽  
Adenosine Triphosphate ◽  
Inorganic Phosphate ◽  
Conformational Coupling ◽  
Rapid Labeling

scholarly journals QUANTITATIVE HISTOCHEMICAL ANALYSIS OF GLYCOLYTIC INTERMEDIATES AND COFACTORS WITH AN OIL WELL TECHNIQUE

1968 ◽  
Vol 16 (1) ◽  
pp. 29-39 ◽  
Author(s):  
F. M. MATSCHINSKY ◽  
J. V. PASSONNEAU ◽  
O. H. LOWRY
Keyword(s):  
Adenosine Triphosphate ◽  
Inorganic Phosphate ◽  
Dry Weight ◽  
Oil Wells ◽  
Oil Well ◽  
Histochemical Analysis ◽  
General Technique ◽  
Enzymatic Cycling ◽  
Glycolytic Intermediates

Quantitative histochemical measurements of glycolytic intermediates and cofactors in samples of the order of 0.1 µg dry weight have been made possible by the use of enzymatic fluorometric methods in combination with the technique of enzymatic cycling. In order to prevent evaporation of the necessarily small volumes of fluid and to avoid exposure to CO2 in the air, incubations are carried out under oil in wells drilled in Teflon. The general technique for conducting analyses in oil wells is described together with specific procedures for measuring glycogen, glucose, glucose 6-phosphate, uridine diphosphoglucose, fructose diphosphate, lactate, adenosine triphosphate, phosphocreatine and inorganic phosphate. The potential of the technique is illustrated by measurements of these compounds in each of nine discrete layers of the retina.

Cerebral high-energy compounds: changes in anoxia

1962 ◽  
Vol 202 (1) ◽  
pp. 77-79 ◽  
Author(s):  
Richard N. Lolley ◽  
Frederick E. Samson
Keyword(s):  
Ion Exchange ◽  
Rat Brain ◽  
Adenosine Triphosphate ◽  
Energy Flow ◽  
Inorganic Phosphate ◽  
High Energy ◽  
High Energy Phosphate ◽  
Guanosine Triphosphate ◽  
Inosine Monophosphate ◽  
Flow Through

Acid-soluble phosphates of rat brain during anoxia were determined by ion-exchange and chemical procedures. There is a general shift during anoxia of triphosphate nucleotides to monophosphates and a very rapid breakdown of phosphoryl-creatine. However, total phosphate leaving the high-energy phosphate pool is not equal to the changes in inorganic phosphate; inorganic phosphate change is much larger than high-energy phosphate change in early anoxia and much smaller in extended anoxia. The patterns of guanosine triphosphate and uridine triphosphate changes are more complex than adenosine triphosphate changes. Nicotinamideadenine dinucleotide levels are steady until late anoxia, at which time they decrease slightly. Cytidine monophosphate is the only cytidine nucleotide detected. Inosine nucleotide concentrations in control animals were below the limit of the method, but in late anoxia inosine monophosphate appeared. The data show that the energy flow through the phosphates in brain is rapid and involves phosphate compounds other than the acid-soluble nucleotides and phosphoryl-creatine.

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