Deoxyribonucleic acid chain growth and organization of replicating units in HeLa cells

Biochemistry ◽  
1977 ◽  
Vol 16 (9) ◽  
pp. 1808-1813 ◽  
Author(s):  
Stephen R. Planck ◽  
Gerald C. Mueller
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Chain Growth

Effects of Irradiation on Synthesis of Deoxyribonucleic Acid and Mitosis in Synchronous Cultures of Hela Cells

Nature ◽  
1966 ◽  
Vol 210 (5034) ◽  
pp. 393-395 ◽  
Author(s):  
T. P. BRENT ◽  
J. A. V. BUTLER ◽  
A. R. CRATHORN
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Synchronous Cultures ◽  
Effects Of Irradiation On

Enzymic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide

Biochemistry ◽  
1969 ◽  
Vol 8 (12) ◽  
pp. 4897-4904 ◽  
Author(s):  
Maurice R. Atkinson ◽  
Murray P. Deutscher ◽  
Arthur Kornberg ◽  
Alan F. Russell ◽  
J. G. Moffatt
Keyword(s):  
Deoxyribonucleic Acid ◽  
Chain Growth ◽  
Enzymic Synthesis

scholarly journals Study upon The Tritiated Thymidine Incorporation into Deoxyribonucleic Acid of Tissue Cultured HeLa Cells

RADIOISOTOPES ◽  
1961 ◽  
Vol 10 (1) ◽  
pp. 135-139
Author(s):  
Toyozo SEKIGUCHI ◽  
Takako KANKURA ◽  
Hideo ETO ◽  
Tetuo IWAKURA
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Thymidine Incorporation ◽  
Tritiated Thymidine

scholarly journals A STUDY OF THE PENETRATION OF MAMMALIAN CELLS BY DEOXYRIBONUCLEIC ACIDS

1961 ◽  
Vol 9 (1) ◽  
pp. 81-91 ◽  
Author(s):  
Ellen Borenfreund ◽  
Aaron Bendich
Keyword(s):  
Nucleic Acids ◽  
Hela Cell ◽  
Perchloric Acid ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Deoxyribonucleic Acid ◽  
Degradation Products ◽  
Dna Bases ◽  
Human Dna ◽  
Nuclear Regions

Tritium-labeled deoxyribonucleic acid (DNA) from pneumococci and from human leukocytes was added to growing cultures of HeLa cells at 37°C. Autoradiography revealed an extensive localization of tritium in the nuclear regions. The label could not be removed by treatment with ribonuclease or dilute perchloric acid, but quantitative removal from the cells could be effected with deoxyribonuclease. Chemical and radioactivity determinations on nucleic acids isolated from the exposed HeLa cells revealed the presence of tritium in all 4 DNA bases. About 12 µg. of tritiated DNA was recovered from 6 x 106 HeLa cells which had been exposed for 24 hours to 240 µg. of the human DNA. From this, it is concluded that the amount of DNA, or its degradation products, taken up by the cells was equivalent to at least 10 per cent of the normal HeLa cell complement.

scholarly journals Effect of Poliovirus on Deoxyribonucleic Acid Synthesis in HeLa Cells

1966 ◽  
Vol 91 (5) ◽  
pp. 1943-1952 ◽  
Author(s):  
W. W. Ackermann ◽  
D. C. Cox ◽  
H. Kurtz ◽  
C. D. Powers ◽  
S. J. Davies
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Deoxyribonucleic Acid Synthesis

scholarly journals SYNTHESIS OF VIRUS-SPECIFIC POLYMERS IN ADENOVIRUS-INFECTED CELLS: EFFECT OF 5-FLUORODEOXYURIDINE

1962 ◽  
Vol 116 (2) ◽  
pp. 141-157 ◽  
Author(s):  
John F. Flanagan ◽  
Harold S. Ginsberg
Keyword(s):  
Hela Cells ◽  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Infectious Virus ◽  
Potent Inhibitor ◽  
Virus Production ◽  
Inhibitory Effect ◽  
Virus Particles ◽  
Specific Proteins ◽  
Infected Cells

Biochemical synthesis in adenovirus-infected HeLa cells was studied utilizing 5-fluorodeoxyuridine (5-FUDR), a potent inhibitor of deoxyribonucleic acid production. Synthesis of saline-soluble DNA and infectious virus was completely suppressed by addition of the analogue to cells as late as 10 hours after infection. The inhibitory effect of this compound was totally reversed by addition of 10–6 M thymidine to the culture medium. Synthesis of DNA essential for virus production began 10 hours after infection and was completed by 16 hours after infection. These data support the hypothesis that the saline-soluble DNA is a precursor of infectious virus particles. Studies of antigen production indicated that formation of virus-specific proteins was directly dependent upon synthesis of DNA.

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