The Isolation and Characterization of a Novel Microsome Fraction from Washed and Detergent-Treated Nuclei of HeLa Cells by the Use of Solutions Containing Deoxyribonucleic Acid*

Biochemistry ◽  
1967 ◽  
Vol 6 (7) ◽  
pp. 1916-1933 ◽  
Author(s):  
Michael K. Bach ◽  
Herbert G. Johnson
Keyword(s):  
Hela Cells ◽  
Deoxyribonucleic Acid ◽  
Isolation And Characterization ◽  
Microsome Fraction

scholarly journals Isolation and Characterization of Teichoic Acid-Like Substance as an Adhesin ofStaphylococcus aureusto HeLa Cells

1996 ◽  
Vol 40 (4) ◽  
pp. 247-254 ◽  
Author(s):  
Takashi Matsuura ◽  
Yoichiro Miyake ◽  
Seiji Nakashima ◽  
Hitoshi Komatsuzawa ◽  
Yasumasa Akagawa ◽  
...  
Keyword(s):  
Hela Cells ◽  
Teichoic Acid ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Thermosensitive Escherichia coli Mutants Defective in Deoxyribonucleic Acid Replication

1973 ◽  
Vol 113 (3) ◽  
pp. 1381-1388 ◽  
Author(s):  
James A. Wechsler ◽  
Volker Nüsslein ◽  
Bernd Otto ◽  
Albrecht Klein ◽  
Friedrich Bonhoeffer ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Deoxyribonucleic Acid ◽  
Isolation And Characterization

scholarly journals Isolation and characterization of the dopa decarboxylase gene of Drosophila melanogaster

1981 ◽  
Vol 1 (6) ◽  
pp. 475-485
Author(s):  
J Hirsh ◽  
N Davidson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Isolation Procedure ◽  
Chromosome Band ◽  
Dopa Decarboxylase ◽  
Developmental Profile ◽  
Isolation And Characterization

We have isolated chromosomal deoxyribonucleic acid clones containing the Drosophila dopa decarboxylase gene. We describe an isolation procedure which can be applied to other nonabundantly expressed Drosophila genes. The dopa decarboxylase gene lies within or very near polytene chromosome band 37C1-2. The gene is interrupted by at least one intron, and the primary mode of regulation is pretranslational. At least two additional sequences hybridized by in vivo ribonucleic acid-derived probes are found within a 35-kilobase region surrounding the gene. The developmental profile of ribonucleic acid transcribed from one of these regions differs from that of the dopa decarboxylase transcript.

scholarly journals Isolation and characterization of cancer stem cells from cervical cancer HeLa cells

2012 ◽  
Vol 64 (4) ◽  
pp. 477-484 ◽  
Author(s):  
Song-Ling Zhang ◽  
Yi-Shu Wang ◽  
Tong Zhou ◽  
Xiao-Wei Yu ◽  
Zhen-Tong Wei ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Stem Cells ◽  
Cancer Stem Cells ◽  
Hela Cells ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Mini-Tn5Km2 Insertion Mutants of Brucella abortus Deficient in Internalization and Intracellular Growth in HeLa Cells

2003 ◽  
Vol 71 (6) ◽  
pp. 3020-3027 ◽  
Author(s):  
Suk Kim ◽  
Masahisa Watarai ◽  
Yuki Kondo ◽  
Janchivdorj Erdenebaatar ◽  
Sou-ichi Makino ◽  
...  
Keyword(s):  
Hela Cells ◽  
Brucella Abortus ◽  
Kanamycin Resistance ◽  
Host Cells ◽  
Class Iii ◽  
Wild Type ◽  
Intracellular Growth ◽  
Isolation And Characterization ◽  
Bacterial Genes

ABSTRACT Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in professional and nonprofessional phagocytes and cause abortion in domestic animals and undulant fever in humans. The mechanism and factors of virulence are not fully understood. To identify genes related to internalization and multiplication in host cells, Brucella abortus was mutagenized by mini-Tn5Km2 transposon that carryied the kanamycin resistance gene, 4,400 mutants were screened, and HeLa cells were infected with each mutant. Twenty-three intracellular-growth-defective mutants were screened and were characterized for internalization and intracellular growth. From these results, we divided the mutants into the following three groups: class I, no internalization and intracellular growth within HeLa cells; class II, an internalization similar to that of the wild type but with no intracellular growth; and class III, internalization twice as high as the wild type but with no intracellular growth. Sequence analysis of DNA flanking the site of transposon showed various insertion sites of bacterial genes that are virulence-associated genes, including virB genes, an ion transporter system, and biosynthesis- and metabolism-associated genes. These internalization and intracellular-growth-defective mutants in HeLa cells also showed defective intracellular growth in macrophages. These results suggest that the virulence-associated genes isolated here contributed to the intracellular growth of both nonprofessional and professional phagocytes.

Sign in / Sign up

Export Citation Format

Share Document