Effect of DNA polymerase inhibitors on DNA repair in intact and permeable human fibroblasts: evidence that DNA polymerases .delta. and .beta. are involved in DNA repair synthesis induced by N-methyl-N'-nitro-N-nitrosoguanidine

Biochemistry ◽  
1990 ◽  
Vol 29 (1) ◽  
pp. 286-291 ◽  
Author(s):  
Russell A. Hammond ◽  
J. Keith McClung ◽  
Michael R. Miller
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Human Fibroblasts ◽  
Dna Polymerases ◽  
Repair Synthesis ◽  
Polymerase Inhibitors ◽  
Dna Repair Synthesis

Involvement of DNA polymerase .delta. in DNA repair synthesis in human fibroblasts at late times after ultraviolet irradiation

Biochemistry ◽  
1988 ◽  
Vol 27 (17) ◽  
pp. 6379-6383 ◽  
Author(s):  
Steven L. Dresler ◽  
Bonnie J. Gowans ◽  
Rona M. Robinson-Hill ◽  
Darel J. Hunting
Keyword(s):  
Dna Repair ◽  
Dna Polymerase ◽  
Ultraviolet Irradiation ◽  
Human Fibroblasts ◽  
Dna Polymerase Delta ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals The Base Excision Repair Pathway in the Nematode Caenorhabditis elegans

2020 ◽  
Vol 8 ◽  
Author(s):  
Noha Elsakrmy ◽  
Qiu-Mei Zhang-Akiyama ◽  
Dindial Ramotar
Keyword(s):  
Dna Repair ◽  
Caenorhabditis Elegans ◽  
Dna Polymerase ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Dna Glycosylases ◽  
Repair Synthesis ◽  
C Elegans ◽  
Base Excision ◽  
Dna Repair Synthesis

Exogenous and endogenous damage to the DNA is inevitable. Several DNA repair pathways including base excision, nucleotide excision, mismatch, homologous and non-homologous recombinations are conserved across all organisms to faithfully maintain the integrity of the genome. The base excision repair (BER) pathway functions to repair single-base DNA lesions and during the process creates the premutagenic apurinic/apyrimidinic (AP) sites. In this review, we discuss the components of the BER pathway in the nematode Caenorhabditis elegans and delineate the different phenotypes caused by the deletion or the knockdown of the respective DNA repair gene, as well as the implications. To date, two DNA glycosylases have been identified in C. elegans, the monofunctional uracil DNA glycosylase-1 (UNG-1) and the bifunctional endonuclease III-1 (NTH-1) with associated AP lyase activity. In addition, the animal possesses two AP endonucleases belonging to the exonuclease-3 and endonuclease IV families and in C. elegans these enzymes are called EXO-3 and APN-1, respectively. In mammalian cells, the DNA polymerase, Pol beta, that is required to reinsert the correct bases for DNA repair synthesis is not found in the genome of C. elegans and the evidence indicates that this role could be substituted by DNA polymerase theta (POLQ), which is known to perform a function in the microhomology-mediated end-joining pathway in human cells. The phenotypes observed by the C. elegans mutant strains of the BER pathway raised many challenging questions including the possibility that the DNA glycosylases may have broader functional roles, as discuss in this review.

Clustered Sites of DNA Repair Synthesis during Early Nucleotide Excision Repair in Ultraviolet Light-Irradiated Quiescent Human Fibroblasts

2002 ◽  
Vol 276 (2) ◽  
pp. 284-295 ◽  
Author(s):  
Maria Svetlova ◽  
Lioudmila Solovjeva ◽  
Nadezhda Pleskach ◽  
Natalia Yartseva ◽  
Tatyana Yakovleva ◽  
...  
Keyword(s):  
Dna Repair ◽  
Nucleotide Excision Repair ◽  
Ultraviolet Light ◽  
Excision Repair ◽  
Human Fibroblasts ◽  
Repair Synthesis ◽  
Nucleotide Excision ◽  
Dna Repair Synthesis

Aphidicolin-sensitive DNA repair synthesis in human fibroblasts damaged with bleomycin is distinct from UV-induced repair

1990 ◽  
Vol 11 (6) ◽  
pp. 1021-1026 ◽  
Author(s):  
Joseph A. DiGiuseppe ◽  
Darel J. Hunting ◽  
Steven L. Dresler
Keyword(s):  
Dna Repair ◽  
Human Fibroblasts ◽  
Repair Synthesis ◽  
Dna Repair Synthesis
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