Bleomycin-induced DNA repair synthesis in permeable human fibroblasts: mediation of long-patch and short-patch repair by distinct DNA polymerases

Biochemistry ◽  
1989 ◽  
Vol 28 (24) ◽  
pp. 9515-9520 ◽  
Author(s):  
Joseph A. DiGiuseppe ◽  
Steven L. Dresler
Keyword(s):  
Dna Repair ◽  
Human Fibroblasts ◽  
Dna Polymerases ◽  
Patch Repair ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

Clustered Sites of DNA Repair Synthesis during Early Nucleotide Excision Repair in Ultraviolet Light-Irradiated Quiescent Human Fibroblasts

2002 ◽  
Vol 276 (2) ◽  
pp. 284-295 ◽  
Author(s):  
Maria Svetlova ◽  
Lioudmila Solovjeva ◽  
Nadezhda Pleskach ◽  
Natalia Yartseva ◽  
Tatyana Yakovleva ◽  
...  
Keyword(s):  
Dna Repair ◽  
Nucleotide Excision Repair ◽  
Ultraviolet Light ◽  
Excision Repair ◽  
Human Fibroblasts ◽  
Repair Synthesis ◽  
Nucleotide Excision ◽  
Dna Repair Synthesis

Aphidicolin-sensitive DNA repair synthesis in human fibroblasts damaged with bleomycin is distinct from UV-induced repair

1990 ◽  
Vol 11 (6) ◽  
pp. 1021-1026 ◽  
Author(s):  
Joseph A. DiGiuseppe ◽  
Darel J. Hunting ◽  
Steven L. Dresler
Keyword(s):  
Dna Repair ◽  
Human Fibroblasts ◽  
Repair Synthesis ◽  
Dna Repair Synthesis

scholarly journals Changes in cyclin/proliferating cell nuclear antigen distribution during DNA repair synthesis.

1988 ◽  
Vol 107 (5) ◽  
pp. 1623-1628 ◽  
Author(s):  
L Toschi ◽  
R Bravo
Keyword(s):  
Dna Repair ◽  
Uv Irradiation ◽  
Proliferating Cell Nuclear Antigen ◽  
Human Fibroblasts ◽  
Immunofluorescent Staining ◽  
Nuclear Antigen ◽  
Nuclear Staining ◽  
Repair Synthesis ◽  
Dna Repair Synthesis ◽  
Proliferating Cell

UV irradiation of quiescent human fibroblasts immediately triggers the appearance of the nuclear protein cyclin/proliferating cell nuclear antigen (PCNA) as detected by indirect immunofluorescent staining after methanol fixation. This was found to be independent of new synthesis of cyclin/PCNA by two-dimensional gel analysis and cycloheximide treatment. The intensity of the immunofluorescent staining of cyclin/PCNA observed in UV-irradiated cells corresponded with the UV dose used and with the DNA repair synthesis detected by autoradiography. The nuclear staining remains as long as DNA repair activity is detected in the cells. By extracting the UV-irradiated quiescent cells with Triton X-100 and fixing with formaldehyde, it was possible to demonstrate by indirect immunofluorescence rapid changes in the cyclin/PCNA population after irradiation, a small proportion (5-10%) of which is tightly associated to the nucleus as determined by high salt extraction. By incubating at low temperature and depleting the ATP pools of the cells before UV irradiation, we have demonstrated that the changes in cyclin/PCNA distribution observed involve at least two different nuclear associations.

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