High-resolution separation and accurate size determination in pulsed-field gel electrophoresis of DNA. 1. DNA size standards and the effect of agarose and temperature

Biochemistry ◽  
1988 ◽  
Vol 27 (26) ◽  
pp. 9204-9210 ◽  
Author(s):  
Mathew K. Mathew ◽  
Cassandra L. Smith ◽  
Charles R. Cantor
Keyword(s):  
High Resolution ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Size Determination ◽  
Pulsed Field ◽  
Dna Size

scholarly journals A regularly spaced DNA size standard with 10 kbp resolution for pulsed field gel electrophoresis

1989 ◽  
Vol 17 (13) ◽  
pp. 5412-5412 ◽  
Author(s):  
Craig A. Cooney ◽  
Jodie L. Galbraith ◽  
E.Morton Bradbury
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Size Standard ◽  
Dna Size

scholarly journals Relation between number of apolipoprotein(a) kringle 4 repeats and mobility of isoforms in agarose gel: basis for a standardized isoform nomenclature

1996 ◽  
Vol 42 (3) ◽  
pp. 436-439 ◽  
Author(s):  
S M Marcovina ◽  
H H Hobbs ◽  
J J Albers
Keyword(s):  
High Resolution ◽  
Sodium Dodecyl Sulfate ◽  
Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Pulsed Field Gel Electrophoresis ◽  
Agarose Gel ◽  
Electrophoretic Method ◽  
Pulsed Field ◽  
Apolipoprotein A ◽  
Respective Number

Abstract The size of apolipoprotein(a) [apo(a)] varies from 187 to 648 kDa because of interallelic differences in the number of kringle 4-encoding sequences in the apo(a) gene. Plasma apo(a) isoform sizes were determined by a high-resolution sodium dodecyl sulfate-agarose gel electrophoretic method, followed by immunoblotting. The relation between the number of apo(a) kringle 4-encoding sequences in the apo(a) gene, as assessed by pulsed-field gel electrophoresis and genomic blotting, and the mobility of apo(a) isoforms in agarose gel was evaluated in 29 individuals who had 48 expressed apo(a) isoforms that contained between 12 and 41 kringle 4 repeats. The log of the kringle number was linearly related to the distance the isoform migrated on the agarose gel (r= 0.999). A nomenclature is proposed to designate apo(a) isoforms by the respective number of kringle 4 repeats.

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