Use of intermediate partitioning to calculate intrinsic isotope effects for the reaction catalyzed by malic enzyme

Biochemistry ◽  
1985 ◽  
Vol 24 (4) ◽  
pp. 944-948 ◽  
Author(s):  
Charles B. Grissom ◽  
W. W. Cleland
Keyword(s):  
Malic Enzyme ◽  
Isotope Effects

Multiple isotope effects with alternative dinucleotide substrates as a probe of the malic enzyme reaction

Biochemistry ◽  
1991 ◽  
Vol 30 (23) ◽  
pp. 5755-5763 ◽  
Author(s):  
Paul M. Weiss ◽  
Sandhya R. Gavva ◽  
Ben G. Harris ◽  
Jeffrey L. Urbauer ◽  
W. W. Cleland ◽  
...  
Keyword(s):  
Malic Enzyme ◽  
Isotope Effects ◽  
Enzyme Reaction

Determination of the Chemical Mechanism of Malic Enzyme by Isotope Effects†

Biochemistry ◽  
1997 ◽  
Vol 36 (5) ◽  
pp. 1141-1147 ◽  
Author(s):  
William A. Edens ◽  
Jeffrey L. Urbauer ◽  
W. W. Cleland
Keyword(s):  
Malic Enzyme ◽  
Isotope Effects ◽  
Chemical Mechanism

Determination of the rate-limiting steps for malic enzyme by the use of isotope effects and other kinetic studies

Biochemistry ◽  
1977 ◽  
Vol 16 (4) ◽  
pp. 571-576 ◽  
Author(s):  
Michael I. Schimerlik ◽  
C. E. Grimshaw ◽  
W. W. Cleland
Keyword(s):  
Malic Enzyme ◽  
Isotope Effects ◽  
Kinetic Studies ◽  
Rate Limiting

Metal Ion Activator on Intrinsic Isotope Effects of Hydride Transfer and Decarboxylation in the Reaction Catalyzed by the NAD-Malic Enzyme from Ascaris suum

Biochemistry ◽  
1995 ◽  
Vol 34 (10) ◽  
pp. 3253-3260 ◽  
Author(s):  
William E. Karsten ◽  
Sandya R. Gavva ◽  
Sang-Hoon Park ◽  
Paul F. Cook
Keyword(s):  
Malic Enzyme ◽  
Metal Ion ◽  
Ascaris Suum ◽  
Isotope Effects ◽  
Hydride Transfer

Determination of the Mechanism of Human Malic Enzyme with Natural and Alternate Dinucleotides by Isotope Effects

2001 ◽  
Vol 396 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Mark A. Rishavy ◽  
Zhiru Yang ◽  
Liang Tong ◽  
W.W. Cleland
Keyword(s):  
Malic Enzyme ◽  
Isotope Effects

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

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