scholarly journals Rates of synthesis and degradation of ribosomal ribonucleic acid during differentiation of Dictyostelium discoideum.

1981 ◽  
Vol 1 (1) ◽  
pp. 35-42 ◽  
Author(s):  
G Mangiarotti ◽  
F Altruda ◽  
H F Lodish
Keyword(s):  
Dictyostelium Discoideum ◽  
Ribonucleic Acid ◽  
Messenger Rna ◽  
Developmental Stages ◽  
Growth Conditions ◽  
Developmentally Regulated ◽  
Rna Translation ◽  
Ribosomal Ribonucleic Acid ◽  
Messenger Rna Translation ◽  
Synthesis And Degradation

Synthesis of ribosomes and ribosomal ribonucleic acid (RNA) continued during differentiation of Dictyostelium discoideum concurrently with extensive turnover of ribosomes synthesized during both growth and developmental stages. We show here that the rate of synthesis of 26S and 17S ribosomal RNA during differentiation was less than 15% of that in growing cells, and by the time of sorocarp formation only about 25% of the cellular ribosomes had been synthesized during differentiation. Ribosomes synthesized during growth and differentiation were utilized in messenger RNA translation to the same extent; about 50% of each class were on polyribosomes. Ribosome degradation is apparently an all-or-nothing process, since virtually all 80S monosomes present in developing cells could be incorporated into polysomes when growth conditions were restored. By several criteria, ribosomes synthesized during growth and differentiation were functionally indistinguishable. Our data, together with previously published information on changes in the messenger RNA population during differentiation, indicate that synthesis of new ribosomes is not necessary for translation of developmentally regulated messenger RNA. We also establish that the overall rate of messenger RNA synthesis during differentiation is less than 15% of that in growing cells.

Rates of synthesis and degradation of ribosomal ribonucleic acid during differentiation of Dictyostelium discoideum

1981 ◽  
Vol 1 (1) ◽  
pp. 35-42
Author(s):  
G Mangiarotti ◽  
F Altruda ◽  
H F Lodish
Keyword(s):  
Dictyostelium Discoideum ◽  
Ribonucleic Acid ◽  
Messenger Rna ◽  
Developmental Stages ◽  
Growth Conditions ◽  
Developmentally Regulated ◽  
Rna Translation ◽  
Ribosomal Ribonucleic Acid ◽  
Messenger Rna Translation ◽  
Synthesis And Degradation

Synthesis of ribosomes and ribosomal ribonucleic acid (RNA) continued during differentiation of Dictyostelium discoideum concurrently with extensive turnover of ribosomes synthesized during both growth and developmental stages. We show here that the rate of synthesis of 26S and 17S ribosomal RNA during differentiation was less than 15% of that in growing cells, and by the time of sorocarp formation only about 25% of the cellular ribosomes had been synthesized during differentiation. Ribosomes synthesized during growth and differentiation were utilized in messenger RNA translation to the same extent; about 50% of each class were on polyribosomes. Ribosome degradation is apparently an all-or-nothing process, since virtually all 80S monosomes present in developing cells could be incorporated into polysomes when growth conditions were restored. By several criteria, ribosomes synthesized during growth and differentiation were functionally indistinguishable. Our data, together with previously published information on changes in the messenger RNA population during differentiation, indicate that synthesis of new ribosomes is not necessary for translation of developmentally regulated messenger RNA. We also establish that the overall rate of messenger RNA synthesis during differentiation is less than 15% of that in growing cells.

scholarly journals AZD1208, a potent and selective pan-Pim kinase inhibitor, demonstrates efficacy in preclinical models of acute myeloid leukemia

Blood ◽  
2014 ◽  
Vol 123 (6) ◽  
pp. 905-913 ◽  
Author(s):  
Erika K. Keeton ◽  
Kristen McEachern ◽  
Keith S. Dillman ◽  
Sangeetha Palakurthi ◽  
Yichen Cao ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Myeloid Leukemia ◽  
Tandem Duplication ◽  
Kinase Inhibitor ◽  
Wild Type ◽  
Internal Tandem Duplication ◽  
Rna Translation ◽  
Key Points ◽  
Messenger Rna Translation ◽  
Flt3 Internal Tandem Duplication

Key Points AZD1208 is a selective pan-Pim kinase inhibitor with efficacy in AML cells, xenografts, and Flt3-internal tandem duplication or Flt3 wild-type patient samples. AML cell growth inhibition is associated with suppression of p70S6K, 4EBP1 phosphorylation, and messenger RNA translation.

scholarly journals Increased Efficiency of Exogenous Messenger RNA Translation in a Krebs Ascites Cell Lysate

1972 ◽  
Vol 69 (5) ◽  
pp. 1299-1303 ◽  
Author(s):  
S. Metafora ◽  
M. Terada ◽  
L. W. Dow ◽  
P. A. Marks ◽  
A. Bank
Keyword(s):  
Messenger Rna ◽  
Ascites Cell ◽  
Rna Translation ◽  
Cell Lysate ◽  
Messenger Rna Translation

Effect of transfer RNA from various sources on placental messenger RNA translation

1983 ◽  
Vol 29 (2) ◽  
pp. 181-195 ◽  
Author(s):  
Susan J. Kelly ◽  
Jean Loria M.T. Gyves ◽  
Judith Ilan
Keyword(s):  
Messenger Rna ◽  
Transfer Rna ◽  
Rna Translation ◽  
Messenger Rna Translation

scholarly journals Interferon Messenger RNA: Translation in Heterologous Cells

1972 ◽  
Vol 69 (5) ◽  
pp. 1203-1207 ◽  
Author(s):  
J. D. Maeyer-Guignard ◽  
E. D. Maeyer ◽  
L. Montagnier
Keyword(s):  
Messenger Rna ◽  
Rna Translation ◽  
Messenger Rna Translation

scholarly journals Deoxyribonucleic acid–ribonucleic acid hybridization. Annealing and quantitative recovery of intact ribosomal ribonucleic acid molecules from hybrids

1969 ◽  
Vol 115 (2) ◽  
pp. 287-294 ◽  
Author(s):  
Michael Fry ◽  
Michael Artman
Keyword(s):  
Ribosomal Rna ◽  
Ribonucleic Acid ◽  
Messenger Rna ◽  
Deoxyribonucleic Acid ◽  
Cellulose Nitrate ◽  
Absolute Size ◽  
Rna Molecules ◽  
Membrane Filters ◽  
Ribosomal Ribonucleic Acid

A simple and efficient method for hybridization and subsequent recovery of non-fragmented ribosomal RNA from the hybrid is described. The procedure involves annealing of immobilized denatured DNA bound on cellulose nitrate membrane filters to complementary RNA in 50% (v/v) formamide–0·33m-potassium chloride–10mm-tris–hydrochloric acid buffer, pH7·4, at 33° for 3hr. Under these conditions no detectable changes in the sedimentation coefficients of the input RNA were detected. The RNA can subsequently be recovered quantitatively from the hybrid in intact form by incubating the filters in formamide or in 85% (v/v) dimethyl sulphoxide. The applicability of the method for the evaluation of the absolute size of ribosomal RNA cistrons in Escherichia coli DNA and for the determination of the size of messenger RNA molecules is discussed.

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