Catalytic Acid−Base Groups in Yeast Pyruvate Decarboxylase. 1. Site-Directed Mutagenesis and Steady-State Kinetic Studies on the Enzyme with the D28A, H114F, H115F, and E477Q Substitutions†

Biochemistry ◽  
2001 ◽  
Vol 40 (25) ◽  
pp. 7355-7368 ◽  
Author(s):  
Min Liu ◽  
Eduard A. Sergienko ◽  
Fusheng Guo ◽  
Jue Wang ◽  
Kai Tittmann ◽  
...  
Keyword(s):  
Steady State ◽  
Pyruvate Decarboxylase ◽  
Kinetic Studies ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Acid Base ◽  
Steady State Kinetic ◽  
State Kinetic

scholarly journals Site-directed mutagenesis and steady-state kinetic analysis of mutant enzymes of human adenylate kinase

IUBMB Life ◽  
1998 ◽  
Vol 46 (4) ◽  
pp. 673-680
Author(s):  
Takanori Ayabe ◽  
Seung Kyu Park ◽  
Hiroyuki Nagahama ◽  
Hideharu Maruyama ◽  
Michihiro Sumida ◽  
...  
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Adenylate Kinase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Steady State Kinetic ◽  
State Kinetic
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