Catalytic Acid−Base Groups in Yeast Pyruvate Decarboxylase. 3. A Steady-State Kinetic Model Consistent with the Behavior of both Wild-Type and Variant Enzymes at All Relevant pH Values†

Biochemistry ◽  
2001 ◽  
Vol 40 (25) ◽  
pp. 7382-7403 ◽  
Author(s):  
Eduard A. Sergienko ◽  
Frank Jordan
Keyword(s):  
Steady State ◽  
Kinetic Model ◽  
Pyruvate Decarboxylase ◽  
Wild Type ◽  
Acid Base ◽  
Ph Values ◽  
Steady State Kinetic ◽  
State Kinetic

scholarly journals Variation in the Steady State Kinetic Parameters of Wild Type and Mutant T5 5′-3′-Exonuclease With pH

1999 ◽  
Vol 274 (25) ◽  
pp. 17711-17717 ◽  
Author(s):  
Timothy J. Pickering ◽  
Scott Garforth ◽  
Jon R. Sayers ◽  
Jane A. Grasby
Keyword(s):  
Steady State ◽  
Kinetic Parameters ◽  
Wild Type ◽  
Steady State Kinetic ◽  
State Kinetic

Serial analysis of renal blood flow by positron tomography with rubidium-82

1986 ◽  
Vol 251 (5) ◽  
pp. H1024-H1030
Author(s):  
N. Tamaki ◽  
C. A. Rabito ◽  
N. M. Alpert ◽  
T. Yasuda ◽  
J. A. Correia ◽  
...  
Keyword(s):  
Blood Flow ◽  
Steady State ◽  
Kinetic Model ◽  
Renal Blood Flow ◽  
Constant Infusion ◽  
Flow Reduction ◽  
Arterial Blood ◽  
Steady State Kinetic ◽  
State Kinetic ◽  
Rubidium 82

To determine whether renal blood flow can be measured by positron-emission tomography (PET) during constant infusion of rubidium-82 (82Rb) using a steady-state kinetic model, studies were performed in 10 dogs at control (n = 10), during mild flow reduction (n = 7), during severe flow reduction (n = 10), and after reperfusion of the kidney (n = 3). PET data were quantified to determine mean concentration of 82Rb (Ct) in each transverse section of the kidney. The arterial concentration (Ca) of 82Rb was measured by well counting of arterial blood samples during the equilibrium scan. 82Rb renal uptake (Ct/Ca) correlated nonlinearly with microsphere renal blood flow according to a steady-state kinetic model (r = 0.90). 82Rb estimated flow was 3.16 +/- 1.36 ml X min-1 X g-1 at control and 1.56 +/- 0.57 and 0.37 +/- 0.59 during mild and severe flow reductions, respectively. Microsphere determined flow was 2.89 +/- 0.77 ml X min-1 X g-1 at control, 1.58 +/- 0.42 at mild reduction, and 0.27 +/- 0.49 at severe reduction. In the occlusion and reperfusion model, the 82Rb estimated flow during occlusion was 0.21 +/- 0.15 ml X min-1 X g-1 and on reperfusion went up to 2.13 +/- 1.08. The contralateral kidney demonstrated reductions in the 82Rb estimated flow of 3.02 +/- 1.62 ml X min-1 X g-1 (63%) and 2.92 +/- 0.89 (61%) during mild and severe flow reductions, respectively. We conclude that PET with 82Rb permits serial quantitative assessment of renal flood flow.

scholarly journals A Pre-Steady State Kinetic Analysis of the αY60W Mutant oftrans-3-Chloroacrylic Acid Dehalogenase: Implications for the Mechanism of the Wild-Type Enzyme

Biochemistry ◽  
2012 ◽  
Vol 51 (46) ◽  
pp. 9420-9435 ◽  
Author(s):  
Jamison P. Huddleston ◽  
Gottfried K. Schroeder ◽  
Kenneth A. Johnson ◽  
Christian P. Whitman
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Wild Type ◽  
Wild Type Enzyme ◽  
Steady State Kinetic ◽  
Chloroacrylic Acid Dehalogenase ◽  
State Kinetic
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