Expression, Site-Directed Mutagenesis, and Steady State Kinetic Analysis of the Terminal Thioesterase Domain of the Methymycin/Picromycin Polyketide Synthase†

Biochemistry ◽  
2002 ◽  
Vol 41 (42) ◽  
pp. 12590-12597 ◽  
Author(s):  
Hongxiang Lu ◽  
Shiou-Chuan Tsai ◽  
Chaitan Khosla ◽  
David E. Cane
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Polyketide Synthase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Steady State Kinetic ◽  
Expression Site ◽  
State Kinetic

scholarly journals Site-directed mutagenesis and steady-state kinetic analysis of mutant enzymes of human adenylate kinase

IUBMB Life ◽  
1998 ◽  
Vol 46 (4) ◽  
pp. 673-680
Author(s):  
Takanori Ayabe ◽  
Seung Kyu Park ◽  
Hiroyuki Nagahama ◽  
Hideharu Maruyama ◽  
Michihiro Sumida ◽  
...  
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Adenylate Kinase ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Steady State Kinetic ◽  
State Kinetic

scholarly journals Tylosin polyketide synthase module 3: stereospecificity, stereoselectivity and steady-state kinetic analysis of β-processing domains via diffusible, synthetic substrates

2015 ◽  
Vol 6 (8) ◽  
pp. 5027-5033 ◽  
Author(s):  
William D. Fiers ◽  
Greg J. Dodge ◽  
Yang Li ◽  
Janet L. Smith ◽  
Robert A. Fecik ◽  
...  
Keyword(s):  
Steady State ◽  
Kinetic Analysis ◽  
Polyketide Synthase ◽  
Ms Analysis ◽  
Steady State Kinetic ◽  
State Kinetic

Natural and modified substrates coupled with LC-MS/MS analysis of products revealed the stereospecificity and stereoselectivity of a polyketide didomain.

Sign in / Sign up

Export Citation Format

Share Document