Isolation of a yeast single-strand deoxyribonucleic acid binding protein that specifically stimulates yeast DNA polymerase I

Biochemistry ◽  
1983 ◽  
Vol 22 (13) ◽  
pp. 3214-3219 ◽  
Author(s):  
Stephen G. LaBonne ◽  
Lawrence B. Dumas
Keyword(s):  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Binding Protein ◽  
Single Strand ◽  
Dna Polymerase I ◽  
Acid Binding Protein ◽  
Polymerase I

scholarly journals The effects of acridine orange on deoxyribonucleic acid in Escherichia coli

1978 ◽  
Vol 171 (3) ◽  
pp. 567-573 ◽  
Author(s):  
G R Barker ◽  
N Hardman
Keyword(s):  
Escherichia Coli ◽  
Acridine Orange ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Insoluble Fraction ◽  
Low Molecular Weight ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Dna Polymerase I ◽  
Polymerase I

1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes.

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