Synthesis by DNA polymerase I on bleomycin-treated deoxyribonucleic acid: a requirement for exonuclease III

Biochemistry ◽  
1981 ◽  
Vol 20 (2) ◽  
pp. 238-244 ◽  
Author(s):  
Osami Niwa ◽  
Robb E. Moses
Keyword(s):  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Dna Polymerase I ◽  
Exonuclease Iii ◽  
Polymerase I

scholarly journals Effects of Vinylphosphonate Internucleotide Linkages on the Cleavage Specificity of Exonuclease III and on the Activity of DNA Polymerase I†

Biochemistry ◽  
2003 ◽  
Vol 42 (11) ◽  
pp. 3239-3246 ◽  
Author(s):  
Zara A. Doddridge ◽  
Richard D. Bertram ◽  
Christopher J. Hayes ◽  
Panos Soultanas
Keyword(s):  
Dna Polymerase ◽  
Dna Polymerase I ◽  
Exonuclease Iii ◽  
Cleavage Specificity ◽  
Polymerase I

scholarly journals The effects of acridine orange on deoxyribonucleic acid in Escherichia coli

1978 ◽  
Vol 171 (3) ◽  
pp. 567-573 ◽  
Author(s):  
G R Barker ◽  
N Hardman
Keyword(s):  
Escherichia Coli ◽  
Acridine Orange ◽  
Dna Polymerase ◽  
Deoxyribonucleic Acid ◽  
Insoluble Fraction ◽  
Low Molecular Weight ◽  
Temperature Sensitive ◽  
Permissive Temperature ◽  
Dna Polymerase I ◽  
Polymerase I

1. Acridine Orange inhibits growth of Escherichia coli K12 when incubated at pH 7.9, but not at pH 7.4.2. At a non-permissive temperature for DNA polymerase I, Acridine Orange inhibits growth of a temperature-sensitive strain and also increases the rate of elimination of the F'-Lac plasmid. 3. DNA isolated from cells treated with Acridine Orange under conditions that inhibit growth contains material of low molecular weight, which is absent from DNA isolated from cells treated under conditions in which growth is not impaired. 4. Cells incubated with Acridine Orange at both pH 7.4 and 7.9 suffer degradation of DNA, as shown by loss of labelled DNA from the acid-insoluble fraction, which is not observed with untreated cells at either pH. 5. The results suggest that elimination of the F'-Lac plasmid by Acridine Orange requires inactivation of repair processes.

scholarly journals Characteristics of DNA polymerase I from an extreme thermophile, Thermus scotoductus strain K1

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Ani Saghatelyan ◽  
Hovik Panosyan ◽  
Armen Trchounian ◽  
Nils‐Kåre Birkeland
Keyword(s):  
Dna Polymerase ◽  
Extreme Thermophile ◽  
Dna Polymerase I ◽  
Polymerase I
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