Specific stimulation of the T7 gene 6 exonuclease by the phage T7 coded deoxyribonucleic acid binding protein

Biochemistry ◽  
1982 ◽  
Vol 21 (23) ◽  
pp. 6000-6005 ◽  
Author(s):  
Linda Roberts ◽  
Paul Sadowski ◽  
J. Tze Fei Wong
Keyword(s):  
Deoxyribonucleic Acid ◽  
Binding Protein ◽  
Acid Binding Protein ◽  
Phage T7 ◽  
Specific Stimulation ◽  
Stimulation Of

Fatty Acid Binding Protein: Stimulation of Microsomal Phosphatidic Acid Formation

1997 ◽  
Vol 341 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Christopher A. Jolly ◽  
Timothy Hubbell ◽  
William D. Behnke ◽  
Friedhelm Schroeder
Keyword(s):  
Fatty Acid ◽  
Phosphatidic Acid ◽  
Fatty Acid Binding Protein ◽  
Binding Protein ◽  
Acid Formation ◽  
Fatty Acid Binding ◽  
Acid Binding Protein ◽  
Stimulation Of

scholarly journals Flow microfluorometric quantitation of the blastogenic response of lymphocytes.

1976 ◽  
Vol 24 (1) ◽  
pp. 383-387 ◽  
Author(s):  
L S Cram ◽  
E R Gomez ◽  
C O Thoen ◽  
J C Forslund ◽  
J H Jett
Keyword(s):  
High Resolution ◽  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Low Salt ◽  
Blastogenic Response ◽  
Flow Microfluorometry ◽  
Specific Stimulation ◽  
Cellular Dna ◽  
Bovine Lymphocytes ◽  
Stimulation Of

A method for quantitating the specific stimulation of peripheral lymphocytes has been developed using the techniques of flow microfluorometry. Peripheral bovine lymphocytes were collected and specifically stained for deoxyribonucleic acid (DNA) content using a low-salt propidium iodide procedure. Flow microfluorometry was used to determine, on the basis of DNA content, the percentage of cells in a population that was stimulated. Extremely uniform staining of the lymphocytes (coefficient of variation of less than 2%) provides a high resolution between proliferating and nonproliferating cells. The method provides a rapid, highly repoducible technique for determing the fraction of lymphocytes stimulated in response to tuberculin antigens based on an increase in cellular DNA content. Specific and nonspecific stimulation by a defined antigen can be measured and resolved.

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