scholarly journals Flow microfluorometric quantitation of the blastogenic response of lymphocytes.

1976 ◽  
Vol 24 (1) ◽  
pp. 383-387 ◽  
Author(s):  
L S Cram ◽  
E R Gomez ◽  
C O Thoen ◽  
J C Forslund ◽  
J H Jett
Keyword(s):  
High Resolution ◽  
Dna Content ◽  
Deoxyribonucleic Acid ◽  
Low Salt ◽  
Blastogenic Response ◽  
Flow Microfluorometry ◽  
Specific Stimulation ◽  
Cellular Dna ◽  
Bovine Lymphocytes ◽  
Stimulation Of

A method for quantitating the specific stimulation of peripheral lymphocytes has been developed using the techniques of flow microfluorometry. Peripheral bovine lymphocytes were collected and specifically stained for deoxyribonucleic acid (DNA) content using a low-salt propidium iodide procedure. Flow microfluorometry was used to determine, on the basis of DNA content, the percentage of cells in a population that was stimulated. Extremely uniform staining of the lymphocytes (coefficient of variation of less than 2%) provides a high resolution between proliferating and nonproliferating cells. The method provides a rapid, highly repoducible technique for determing the fraction of lymphocytes stimulated in response to tuberculin antigens based on an increase in cellular DNA content. Specific and nonspecific stimulation by a defined antigen can be measured and resolved.

scholarly journals Measurement of cellular DNA mass by flow microfluorometry with use of a biological internal standard.

1978 ◽  
Vol 26 (2) ◽  
pp. 145-148 ◽  
Author(s):  
E Tannenbaum ◽  
M Cassidy ◽  
O Alabaster ◽  
C Herman
Keyword(s):  
Dna Content ◽  
Internal Standard ◽  
Cell Populations ◽  
Experimental Cell ◽  
Flow Microfluorometry ◽  
Chicken Erythrocytes ◽  
Cellular Dna ◽  
Biological Standard

Use of a biological standard (chicken erythrocytes) mixed with experimental cell populations allows control of all variables in flow microfluorometric determination of DNA content. These variables include both staining and instrument procedures. In addition, the use of a biological standard allows determination of cellular DNA mass in unperturbed cell populations. DNA mass measured by FMF technique correlates closely with values reported in the literature that used biochemical techniques.

scholarly journals Quantation by flow microfluorometry of total cellular DNA in Acanthamoeba.

1978 ◽  
Vol 26 (9) ◽  
pp. 713-718 ◽  
Author(s):  
P B Coulson ◽  
R Tyndall
Keyword(s):  
Dna Content ◽  
Acanthamoeba Castellanii ◽  
Amoeba Proteus ◽  
Acanthamoeba Polyphaga ◽  
Dna Values ◽  
Flow Microfluorometry ◽  
Identification Of Species ◽  
Acanthamoeba Culbertsoni ◽  
Cellular Dna

The DNA content of five species of Acanthamoeba was determined by flow microfluorometry. Acanthamoeba castellanii (AC-30), acanthamoeba polyphaga (APG and P-23), acanthamoeba rhysodes, acanthamoeba culbertsoni (A-1), and acanthamoeba royreba were grown in a casitone based medium 24-48 HR. The trophozoites were harvested, and evaluated for DNA-bound fluorescence. All species tested has DNA values between 2.0-5.0 pg/cell. These results placed DNA/cell values of Acanthamoeba slightly lower than DNA/cell values of other eucaryotic cells and much lower than Amoeba proteus values. These results indicate that FMF may be a useful adjunct in distinguishing Acanthamoeba cells from either eucaryotic cells or some other amoeba. However, differences in DNA/cell between species of Acanthamoeba are small and would not be useful in identification of species.

Antigen-specific stimulation and trans-stimulation of T cells in long-term culture

1979 ◽  
Vol 9 (9) ◽  
pp. 665-670 ◽  
Author(s):  
Andrei A. Augustin ◽  
Michael H. Julius ◽  
Humberto Cosenza
Keyword(s):  
T Cells ◽  
Long Term Culture ◽  
Specific Stimulation ◽  
Stimulation Of
Sign in / Sign up

Export Citation Format

Share Document