Mechanism of lactose transport in Escherichia coli membrane vesicles: evidence for the involvement of a histidine residue(s) in the response of the lac carrier to the proton electrochemical gradient

Biochemistry ◽  
1982 ◽  
Vol 21 (23) ◽  
pp. 5800-5805 ◽  
Author(s):  
Maria Luisa Garcia ◽  
Lekha Patel ◽  
Etana Padan ◽  
H. Ronald Kaback
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Histidine Residue ◽  
Electrochemical Gradient ◽  
Lactose Transport

The action of tributyltin chloride on the uptake of proline and glutamine by intact cells of Escherichia coli

1979 ◽  
Vol 57 (12) ◽  
pp. 1376-1383 ◽  
Author(s):  
A. P. Singh ◽  
P. D. Bragg
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Lipoic Acid ◽  
Membrane Vesicles ◽  
Electrochemical Gradient ◽  
Intact Cells ◽  
Iodide Ions ◽  
Tributyltin Chloride ◽  
Anion Exchange Reaction ◽  
Glutamine Uptake

Tributyltin chloride inhibits growth and uptake of glutamine and proline into intact cells of Escherichia coli. It causes efflux of the accumulated amino acids. A pH gradient generated in intact cells and everted membrane vesicles is dissipated by this compound. These effects do not require lipoic acid but are dependent on the presence of chloride, bromide, or iodide ions. We conclude that tributyltin chloride can catalyse a transmembrane OH−–anion exchange reaction and that this is its mode of inhibition of the uptake of these amino acids. The response of proline and glutamine uptake to the inhibitor is similar and is consistent with the transport of both amino acids requiring an electrochemical gradient of protons.

scholarly journals Energy Coupling in Membrane Vesicles of Escherichia coli

1974 ◽  
Vol 249 (9) ◽  
pp. 2939-2945
Author(s):  
Hajime Hirata ◽  
Karlheinz Altendorf ◽  
Franklin M. Harold
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Energy Coupling
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