Direct measurement of lactose/proton symport in Escherichia coli membrane vesicles: further evidence for the involvement of a histidine residue(s)

Biochemistry ◽  
1982 ◽  
Vol 21 (23) ◽  
pp. 5805-5810 ◽  
Author(s):  
Lekha Patel ◽  
Maria Luisa Garcia ◽  
H. Ronald Kaback
Keyword(s):  
Escherichia Coli ◽  
Direct Measurement ◽  
Membrane Vesicles ◽  
Histidine Residue ◽  
Proton Symport

scholarly journals Energy Coupling in Membrane Vesicles of Escherichia coli

1974 ◽  
Vol 249 (9) ◽  
pp. 2939-2945
Author(s):  
Hajime Hirata ◽  
Karlheinz Altendorf ◽  
Franklin M. Harold
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Energy Coupling

scholarly journals Effect of lipids on the reconstitution of D-lactate oxidase in Escherichia coli membrane vesicles.

1976 ◽  
Vol 251 (21) ◽  
pp. 6662-6666
Author(s):  
C George-Nascimento ◽  
S J Wakil ◽  
S A Short ◽  
H R Kaback
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles ◽  
Lactate Oxidase

scholarly journals Anaerobic transport in Escherichia coli membrane vesicles.

1975 ◽  
Vol 250 (17) ◽  
pp. 6792-6798
Author(s):  
J Boonstra ◽  
M T Huttunen ◽  
W N Konings
Keyword(s):  
Escherichia Coli ◽  
Membrane Vesicles

scholarly journals Improving cell-free glycoprotein synthesis by characterizing and enriching native membrane vesicles

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jasmine M. Hershewe ◽  
Katherine F. Warfel ◽  
Shaelyn M. Iyer ◽  
Justin A. Peruzzi ◽  
Claretta J. Sullivan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Synthetic Biology ◽  
Membrane Protein ◽  
Membrane Vesicles ◽  
Processing Methods ◽  
Glycoprotein Synthesis ◽  
On Demand ◽  
Free Gene ◽  
Membrane Bound

AbstractCell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by characterizing native membrane vesicles in Escherichia coli-based CFE extracts and describing methods to enrich vesicles with heterologous, membrane-bound machinery. As a model, we focus on bacterial glycoengineering. We first use multiple, orthogonal techniques to characterize vesicles and show how extract processing methods can be used to increase concentrations of membrane vesicles in CFE systems. Then, we show that extracts enriched in vesicle number also display enhanced concentrations of heterologous membrane protein cargo. Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free N-linked and O-linked glycoprotein synthesis. We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities.

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