Effect of the proton electrochemical gradient on maleimide inactivation of active transport in Escherichia coli membrane vesicles

Biochemistry ◽  
1981 ◽  
Vol 20 (11) ◽  
pp. 3308-3313 ◽  
Author(s):  
Dunell E. Cohn ◽  
Gregory J. Kaczorowski ◽  
H. Ronald Kaback
Keyword(s):  
Escherichia Coli ◽  
Active Transport ◽  
Membrane Vesicles ◽  
Electrochemical Gradient

Spin-label study of energy-coupled active transport in Escherichia coli membrane vesicles

Biochemistry ◽  
1974 ◽  
Vol 13 (25) ◽  
pp. 5210-5214 ◽  
Author(s):  
Joseph J. Baldassare ◽  
Dan E. Robertson ◽  
Alice G. McAfee ◽  
Chien Ho
Keyword(s):  
Escherichia Coli ◽  
Active Transport ◽  
Spin Label ◽  
Membrane Vesicles ◽  
Label Study

The action of tributyltin chloride on the uptake of proline and glutamine by intact cells of Escherichia coli

1979 ◽  
Vol 57 (12) ◽  
pp. 1376-1383 ◽  
Author(s):  
A. P. Singh ◽  
P. D. Bragg
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Lipoic Acid ◽  
Membrane Vesicles ◽  
Electrochemical Gradient ◽  
Intact Cells ◽  
Iodide Ions ◽  
Tributyltin Chloride ◽  
Anion Exchange Reaction ◽  
Glutamine Uptake

Tributyltin chloride inhibits growth and uptake of glutamine and proline into intact cells of Escherichia coli. It causes efflux of the accumulated amino acids. A pH gradient generated in intact cells and everted membrane vesicles is dissipated by this compound. These effects do not require lipoic acid but are dependent on the presence of chloride, bromide, or iodide ions. We conclude that tributyltin chloride can catalyse a transmembrane OH−–anion exchange reaction and that this is its mode of inhibition of the uptake of these amino acids. The response of proline and glutamine uptake to the inhibitor is similar and is consistent with the transport of both amino acids requiring an electrochemical gradient of protons.

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