Derivatized Mesoporous Silica Beads for MALDI-TOF MS Profiling of Human Plasma and Urine

2009 ◽  
Vol 20 (5) ◽  
pp. 913-923 ◽  
Author(s):  
Rosa Terracciano ◽  
Luigi Pasqua ◽  
Francesca Casadonte ◽  
Stella Frascà ◽  
Mariaimmacolata Preianò ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Human Plasma ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Human Plasma And Urine ◽  
Silica Beads ◽  
Tof Ms

Peptidome profiling of induced sputum by mesoporous silica beads and MALDI-TOF MS for non-invasive biomarker discovery of chronic inflammatory lung diseases

PROTEOMICS ◽  
2011 ◽  
Vol 11 (16) ◽  
pp. 3402-3414 ◽  
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Grazia P. Palladino ◽  
Giovanna E. Carpagnano ◽  
Maria P. Foschino Barbaro ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Lung Diseases ◽  
Biomarker Discovery ◽  
Induced Sputum ◽  
Maldi Tof Ms ◽  
Non Invasive ◽  
Maldi Tof ◽  
Silica Beads ◽  
Tof Ms

scholarly journals Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2311
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Corrado Pelaia ◽  
Rocco Savino
Keyword(s):  
Synovial Fluid ◽  
Mesoporous Silica ◽  
Sample Preparation ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Versatile Tool ◽  
Tof Ms

Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

A novel voltammetry offline coupled MALDI/TOF MS characterization of electrochemical reaction products and the voltammetric determination of febuxostat in human plasma

Talanta ◽  
2019 ◽  
Vol 194 ◽  
pp. 542-547 ◽  
Author(s):  
Fawzi A. El-Yazbi ◽  
Omayma A. Amin ◽  
Rania Bakry ◽  
Essam F. Khamis ◽  
Eman I. El-Kimary ◽  
...  
Keyword(s):  
Human Plasma ◽  
Electrochemical Reaction ◽  
Voltammetric Determination ◽  
Reaction Products ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Epitope-imprinted mesoporous silica nanoparticles for specific recognition of tyrosine phosphorylation

2019 ◽  
Vol 55 (67) ◽  
pp. 9927-9930 ◽  
Author(s):  
Guiyuan Zhang ◽  
Liyan Jiang ◽  
Juntao Zhou ◽  
Lianghai Hu ◽  
Shouhua Feng
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Tyrosine Phosphorylation ◽  
Mesoporous Silica Nanoparticles ◽  
Molecularly Imprinted ◽  
Maldi Tof Ms ◽  
Specific Recognition ◽  
Maldi Tof ◽  
Phosphorylated Peptides ◽  
Tof Ms

Molecularly imprinted mesoporous silica nanoparticles were synthesized to specifically enrich tyrosine phosphorylated peptides followed by MALDI-TOF MS detection.

Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization

Talanta ◽  
2010 ◽  
Vol 80 (4) ◽  
pp. 1532-1538 ◽  
Author(s):  
Rosa Terracciano ◽  
Francesca Casadonte ◽  
Luigi Pasqua ◽  
Patrizio Candeloro ◽  
Enzo Di Fabrizio ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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