Epitope-imprinted mesoporous silica nanoparticles for specific recognition of tyrosine phosphorylation

2019 ◽  
Vol 55 (67) ◽  
pp. 9927-9930 ◽  
Author(s):  
Guiyuan Zhang ◽  
Liyan Jiang ◽  
Juntao Zhou ◽  
Lianghai Hu ◽  
Shouhua Feng
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Tyrosine Phosphorylation ◽  
Mesoporous Silica Nanoparticles ◽  
Molecularly Imprinted ◽  
Maldi Tof Ms ◽  
Specific Recognition ◽  
Maldi Tof ◽  
Phosphorylated Peptides ◽  
Tof Ms

Molecularly imprinted mesoporous silica nanoparticles were synthesized to specifically enrich tyrosine phosphorylated peptides followed by MALDI-TOF MS detection.

scholarly journals Hexagonal Mesoporous Silica as a Rapid, Efficient and Versatile Tool for MALDI-TOF MS Sample Preparation in Clinical Peptidomics Analysis: A Pilot Study

Molecules ◽  
2019 ◽  
Vol 24 (12) ◽  
pp. 2311
Author(s):  
Rosa Terracciano ◽  
Mariaimmacolata Preianò ◽  
Giuseppina Maggisano ◽  
Corrado Pelaia ◽  
Rocco Savino
Keyword(s):  
Synovial Fluid ◽  
Mesoporous Silica ◽  
Sample Preparation ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Versatile Tool ◽  
Tof Ms

Improvement in high-throughput MALDI-TOF MS analysis requires practical and efficient sample preparation protocols for high acquisition rates. The use of hexagonal mesoporous silica (HMS) sorbents in combination with MALDI-TOF MS was explored as a versatile tool for peptidomic profiling of clinical specimens difficult to process, but considered important sources of disease biomarkers: synovial fluid and sputum. A rapid and efficient procedure, based on dispersive solid-phase extraction of peptides using commercially available wormhole mesostructured HMS, was tested for: a) pre-concentration of standard peptides in serially diluted solution up to the sub-nanomolar range; b) peptidome profiling of sputum and synovial fluid. The use of HMS, as dispersed sponges, significantly amplified the peptidic repertoire of sputum and synovial fluid by excluding from the adsorptive process large size proteins, which mask and/or suppress peptidome signals. The protocol proposed, as dispersive solid phase extraction, ensures good analytical performances. Moreover, it is economical and rapid, as it avoids the use of less reproducible and prolonged sample preparation procedures, such as the use of ultrafiltration filter devices. These findings may contribute to defining a high-throughput screening MS-based platform for monitoring key peptidic features of difficult to analyse bodily fluids in a clinical setting.

Simultaneous Analysis of Phosphorylated Peptides by MALDI-TOF-MS

2006 ◽  
Vol 63 (11-12) ◽  
pp. 595-598 ◽  
Author(s):  
J. -H. Kang ◽  
R. Toita ◽  
Y. Jiang ◽  
T. Niidome ◽  
Y. Katayama
Keyword(s):  
Simultaneous Analysis ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Phosphorylated Peptides ◽  
Tof Ms

Selective enrichment of proteins for MALDI-TOF MS analysis based on molecular imprinting

2015 ◽  
Vol 51 (17) ◽  
pp. 3541-3544 ◽  
Author(s):  
Wei Wan ◽  
Qiang Han ◽  
Xiaoqiong Zhang ◽  
Yuming Xie ◽  
Jieping Sun ◽  
...  
Keyword(s):  
Molecular Imprinting ◽  
Molecularly Imprinted Polymers ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Maldi Tof Ms ◽  
Selective Enrichment ◽  
Maldi Tof ◽  
Ms Analysis ◽  
First Time ◽  
Tof Ms

Molecularly imprinted polymers were applied for selective enrichment of proteins in MALDI-TOF MS analysis for the first time.

Enhancing plasma peptide MALDI-TOF-MS profiling by mesoporous silica assisted crystallization

Talanta ◽  
2010 ◽  
Vol 80 (4) ◽  
pp. 1532-1538 ◽  
Author(s):  
Rosa Terracciano ◽  
Francesca Casadonte ◽  
Luigi Pasqua ◽  
Patrizio Candeloro ◽  
Enzo Di Fabrizio ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Derivatized Mesoporous Silica Beads for MALDI-TOF MS Profiling of Human Plasma and Urine

2009 ◽  
Vol 20 (5) ◽  
pp. 913-923 ◽  
Author(s):  
Rosa Terracciano ◽  
Luigi Pasqua ◽  
Francesca Casadonte ◽  
Stella Frascà ◽  
Mariaimmacolata Preianò ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Human Plasma ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Human Plasma And Urine ◽  
Silica Beads ◽  
Tof Ms

scholarly journals Specific On-Plate Enrichment of Phosphorylated Peptides for Direct MALDI-TOF MS Analysis

2007 ◽  
Vol 6 (12) ◽  
pp. 4763-4769 ◽  
Author(s):  
Liang Qiao ◽  
Christophe Roussel ◽  
Jingjing Wan ◽  
Pengyuan Yang ◽  
Hubert H. Girault ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Phosphorylated Peptides ◽  
Tof Ms
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