A Biodegradable Low Molecular Weight Polyethylenimine Derivative as Low Toxicity and Efficient Gene Vector

2009 ◽  
Vol 20 (2) ◽  
pp. 322-332 ◽  
Author(s):  
Yuting Wen ◽  
Shirong Pan ◽  
Xin Luo ◽  
Xuan Zhang ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Efficient Gene ◽  
Low Toxicity

scholarly journals Binding Affinity, Cellular Uptake, and Subsequent Intracellular Trafficking of the Nano-Gene Vector P123-PEI-R13

2016 ◽  
Vol 2016 ◽  
pp. 1-8
Author(s):  
Yaguang Zhang ◽  
Hongmei Shu ◽  
Jing Hu ◽  
Min Zhang ◽  
Junweng Wu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Cellular Uptake ◽  
Intracellular Trafficking ◽  
Transfection Efficiency ◽  
Cross Linking ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Dna Complexes ◽  
Dynamic Source

A nano-gene vector PEI-P123-R13 was synthesized by cross-linking low molecular weight PEI with P123 and further coupling bifunctional peptide R13 to the polymer for targeting tumor and increasing cellular uptake. The binding assessment of R13 toαvβ3 positive cells was performed by HRP labeling. The internalization pathways of P123-PEI-R13/DNA complexes were investigated based on the effect of specific endocytic inhibitors on transfection efficiency. The mechanism of intracellular trafficking was investigated based on the effect of endosome-lysosome acidification inhibitors, cytoskeleton, and dynein inhibitors on transfection efficiency. The results indicated that the bifunctional peptide R13 had the ability of binding toαvβ3 positive cellsin vitro. The modification of P123-PEI-R13 with R13 made it display new property of internalization. P123-PEI-R13/DNA complexes were conducted simultaneously via clathrin-mediated endocytosis, caveolin-mediated endocytosis, macropinocytosis, and possible energy-independent route. After internalization, P123-PEI-R13/DNA complexes could escape from the endosome-lysosome system because of its acidification and further took microtubule as the track and dynein as the dynamic source to be transported toward the microtubule (+) end, to wit nucleus, under the action of microfilament, and with the aid of intermediate filament.

Low Molecular Weight Polyethylenimine Conjugated Gold Nanoparticles as Efficient Gene Vectors

2010 ◽  
Vol 21 (5) ◽  
pp. 836-843 ◽  
Author(s):  
Chu Hu ◽  
Qi Peng ◽  
Fujie Chen ◽  
Zhenlin Zhong ◽  
Renxi Zhuo
Keyword(s):  
Molecular Weight ◽  
Gold Nanoparticles ◽  
Low Molecular Weight ◽  
Efficient Gene ◽  
Gene Vectors

Dihalogen and Solvent-Free Preparation of syn-Bimane

Synlett ◽  
2018 ◽  
Vol 29 (08) ◽  
pp. 1043-1046 ◽  
Author(s):  
Flavio Grynszpan ◽  
Ishita Neogi ◽  
Partha Das
Keyword(s):  
Molecular Weight ◽  
Molecular Probes ◽  
Solvent Free ◽  
Low Molecular Weight ◽  
Laser Dyes ◽  
Practical Applications ◽  
Chlorine Gas ◽  
Low Toxicity

Fluorescent bimanes are low molecular weight and low toxicity molecules with applications ranging from biology to LASER dyes. The widespread use of these molecular probes has presumably been stalled by the hazards involved in their current synthetic preparation which involve handling of dangerous halogens like chlorine (gas) and bromine (liq.). The accessibility achieved by the simple and safe dihalogen and solvent-free methodologies described here open the floodgates to additional future practical applications of bimanes.

A core–shell structured polyplex for efficient and non-toxic gene delivery

2017 ◽  
Vol 5 (26) ◽  
pp. 5101-5108 ◽  
Author(s):  
Saisai Wang ◽  
Fei Wang ◽  
Qiang Zhang ◽  
Yiyun Cheng
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Core Shell ◽  
Low Molecular Weight ◽  
The Core ◽  
Transfection Efficacy ◽  
Toxic Gene ◽  
Low Toxicity ◽  
Linear Polyethylenimine

We developed a core–shell polyplex with minimal high generation dendrimer to condense DNA and low-molecular-weight linear polyethylenimine coated on the core. The polyplex represented both high transfection efficacy and low toxicity.

N-Succinyl-chitosan grafted with low molecular weight polyethylenimine as a serum-resistant gene vector

2009 ◽  
Vol 5 (6) ◽  
pp. 629 ◽  
Author(s):  
Bo Lu ◽  
Yun-Xia Sun ◽  
Yun-Qiu Li ◽  
Xian-Zheng Zhang ◽  
Ren-Xi Zhuo
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Gene Vector ◽  
Resistant Gene

Starburst low-molecular weight polyethylenimine for efficient gene delivery

2011 ◽  
Vol 100A (1) ◽  
pp. 134-140 ◽  
Author(s):  
Yanjun Zhao ◽  
Rulei Yang ◽  
Dong Liu ◽  
Mingjing Sun ◽  
Lijun Zhou ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Low Molecular Weight ◽  
Efficient Gene

Carboxyimamidate, a low-molecular-weight polyelectrolyte with antitumor properties and low toxicity

1982 ◽  
Vol 25 (9) ◽  
pp. 1060-1064 ◽  
Author(s):  
Joseph E. Fields ◽  
Samuel S. Asculai ◽  
John H. Johnson ◽  
Randall K. Johnson
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Low Toxicity ◽  
Antitumor Properties
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