Covalent Cell Surface Functionalization of Human Fetal Osteoblasts for Tissue Engineering

2011 ◽  
Vol 22 (7) ◽  
pp. 1422-1432 ◽  
Author(s):  
Françoise Borcard ◽  
Aurélien Godinat ◽  
Davide Staedler ◽  
Horacio Comas Blanco ◽  
Anne-Laure Dumont ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Surface ◽  
Surface Functionalization

Cell Surface Functionalization with Heparin‐Conjugated Lipid to Suppress Blood Activation

2021 ◽  
pp. 2008167
Author(s):  
Kenta Asawa ◽  
Kazuhiko Ishihara ◽  
Kristina N. Ekdahl ◽  
Bo Nilsson ◽  
Yuji Teramura
Keyword(s):  
Cell Surface ◽  
Surface Functionalization

288 CHARACTERIZATION OF PORCINE ADIPOSE TISSUE-DERIVED ADULT STEM CELL SURFACE PROTEINS

2009 ◽  
Vol 21 (1) ◽  
pp. 241
Author(s):  
K. J. Williams ◽  
R. A. Godke ◽  
K. R. Bondioli
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Cell Surface ◽  
Cell Lines ◽  
Adult Stem Cell ◽  
Surface Protein ◽  
Surface Proteins ◽  
Early Passage ◽  
Cell Surface Proteins

Human adipose tissue-derived adult stem (ADAS) cells are a self-renewing population of cells with a multilineage plasticity similar to bone marrow-derived mesenchymal stem cells. Human ADAS have promise for use in combination with various biomaterials for reconstructive tissue engineering. The phenotypic profile of human ADAS cell surface proteins has been partially characterized for stem cell-associated cluster differentiation molecules including CD29, CD44, and CD90. Porcine ADAS cells, an animal model for tissue engineering, also have the ability to self-renew and differentiate into multiple tissue lineages. However, the surface protein phenotype has not been described. Because porcine ADAS are isolated from fat depots likely different from human ADAS liposuction aspirates, it is important to characterize these cells. In this study, we have partially characterized the surface protein phenotype of undifferentiated porcine ADAS cells in comparison with the immunophenotype of undifferentiated human ADAS cells as reported in the literature. Flow cytometry and enhanced chemiluminescence Western blot analysis of early passage (passages 0–4) porcine ADAS cell populations demonstrated that the profiles are not similar to the human ADAS cell surface. Immunoblot detection paired with an enhanced chemiluminescence kit revealed a positive expression for CD44 and CD90 in human ADAS cells as indicated by bands present at the expected sizes and a negative expression for CD44 and CD90 in porcine ADAS cells. Flow cytometric analysis also indicated differences between human and early passage porcine ADAS cell surfaces with a relatively low expression of CD29 (5 cell lines with a mean percent positive of 4.5 ± 1.7 and a range of 2.5–7.2%) and CD44 (5 cell lines with a mean percent positive of 0.66 ± 0.67 and a range of 0.0–1.8%) compared with human ADAS values of 98 ± 1 and 60 ± 15, respectively (Gronthos et al. 2001). Other cell surface proteins analyzed at early passages include CD3 (3 cell lines; 0.07 ± 0.06% positive and 0.0–0.1 range), CD8 (3 cell lines; 0.10 ± 0.10% positive and 0–0.2 range), and CD90 [5 cell lines; 12.7 ± 11.9% positive and 2.4–33 range; human ADAS geometric mean 25.96% (Zuk et al. 2002)]. Analysis of late passage (passages 5–11) porcine ADAS cell populations revealed an increased expression of CD29 (3 cell lines; 26.4 ± 7.2% positive and 21.2–34.6 range). The expression level of CD90 at late passages were 21.3 and 26.9% positive for 2 cell lines and CD44 remained low (3 cell lines; 4.1 ± 3.5% positive and 0.2–7.0 range). Later passages were also analyzed for c-Kit (CD117), which was expressed at low levels (2 cell lines; 0.3 and 0.4% positive). The characterization of adipose tissue-derived adult stem cell surface proteins present at different stages of in vitro culture from a model animal, such as the pig, could have valuable impacts on tissue engineering research. These results suggest that care should be taken when interpreting results from animal models of somatic stem cells.

scholarly journals Nanocomposite Scaffold for Chondrocyte Growth and Cartilage Tissue Engineering: Effects of Carbon Nanotube Surface Functionalization

2014 ◽  
Vol 20 (17-18) ◽  
pp. 2305-2315 ◽  
Author(s):  
Nadeen O. Chahine ◽  
Nicole M. Collette ◽  
Cynthia B. Thomas ◽  
Damian C. Genetos ◽  
Gabriela G. Loots
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Surface Functionalization ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Nanocomposite Scaffold ◽  
And Cartilage

scholarly journals Electrospun Nanofibers Applications in Dentistry

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Seog-Jin Seo ◽  
Hae-Won Kim ◽  
Jung-Hwan Lee
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Pore Size ◽  
Surface Functionalization ◽  
High Surface ◽  
High Surface Area ◽  
Electrospun Nanofibers ◽  
Fiber Alignment ◽  
Cell Homing ◽  
Polymeric Nanofibers

Nanofibrous structures exhibit many interesting features, such as high surface area and surface functionalization and porosity in the range from submicron to nanoscale, which mimics the natural extracellular matrix. In particular, electrospun nanofibers have gained great attention in the field of tissue engineering due to the ease of fabrication and tailorability in pore size, scaffold shape, and fiber alignment. For the reasons, recently, polymeric nanofibers or bioceramic nanoparticle-incorporated nanofibers have been used in dentistry, and their nanostructure and flexibility have contributed to highly promotive cell homing behaviors, resulting in expecting improved dental regeneration. Here, this paper focuses on recently applied electrospun nanofibers in dentistry in the range from the process to the applications.

Surface functionalization of dual growth factor on hydroxyapatite-coated nanofibers for bone tissue engineering

2020 ◽  
Vol 520 ◽  
pp. 146311
Author(s):  
Nopphadol Udomluck ◽  
Haram Lee ◽  
Seungpyo Hong ◽  
Soo-Hong Lee ◽  
Hansoo Park
Keyword(s):  
Tissue Engineering ◽  
Growth Factor ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Surface Functionalization

scholarly journals Hydrophilic Surface Functionalization of Electrospun Nanofibrous Scaffolds in Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2636
Author(s):  
Beata Niemczyk-Soczynska ◽  
Arkadiusz Gradys ◽  
Pawel Sajkiewicz
Keyword(s):  
Tissue Engineering ◽  
Surface Functionalization ◽  
Physical Adsorption ◽  
Electrospun Nanofibers ◽  
Biologically Active ◽  
Bioactive Molecules ◽  
Layer By Layer ◽  
Hydrophilic Surface ◽  
Nanofibrous Scaffolds ◽  
Covalent Grafting

Electrospun polymer nanofibers have received much attention in tissue engineering due to their valuable properties such as biocompatibility, biodegradation ability, appropriate mechanical properties, and, most importantly, fibrous structure, which resembles the morphology of extracellular matrix (ECM) proteins. However, they are usually hydrophobic and suffer from a lack of bioactive molecules, which provide good cell adhesion to the scaffold surface. Post-electrospinning surface functionalization allows overcoming these limitations through polar groups covalent incorporation to the fibers surface, with subsequent functionalization with biologically active molecules or direct deposition of the biomolecule solution. Hydrophilic surface functionalization methods are classified into chemical approaches, including wet chemical functionalization and covalent grafting, a physiochemical approach with the use of a plasma treatment, and a physical approach that might be divided into physical adsorption and layer-by-layer assembly. This review discusses the state-of-the-art of hydrophilic surface functionalization strategies of electrospun nanofibers for tissue engineering applications. We highlighted the major advantages and drawbacks of each method, at the same time, pointing out future perspectives and solutions in the hydrophilic functionalization strategies.

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